Molecular Characterization of <i>CYP73A9</i> and<i>CYP82A1</i> P450 Genes Involved in Plant Defense in Pea

Whitbred, Joy; Schuler, Mary A.

doi:10.1104/pp.124.1.47

Cited by 69 publications

(33 citation statements)

References 43 publications

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“…Furthermore, the effects of increased Phe availability on enhancing both PAL and 4CL transcript levels may also be consistent with known similarities between PAL and 4CL promoter regions, i.e. in terms of their cis-regulatory elements, which include P, A, and L boxes (48). The presence of similar regulatory elements in both of the promoter regions thus strengthens further the hypothesis that transcriptional control is being exercised in a similar manner, enabling a coordinated pattern of induction.…”

Section: Table I Primers and Probes Used For Cloning And Quantitativesupporting

confidence: 80%

Transcriptional Control of Monolignol Biosynthesis in Pinus taeda

Anterola

Jeon

Davin

et al. 2002

Journal of Biological Chemistry

125

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Transcriptional profiling of the phenylpropanoid pathway in Pinus taeda cell suspension cultures was carried out using quantitative real time PCR analyses of all known genes involved in the biosynthesis of the two monolignols, p-coumaryl and coniferyl alcohols (lignin/ lignan precursors). When the cells were transferred to a medium containing 8% sucrose and 20 mM potassium iodide, the monolignol/phenylpropanoid pathway was induced, and transcript levels for phenylalanine ammonia lyase, cinnamate 4-hydroxylase, p-coumarate 3-hydroxylase, 4-coumarate:CoA ligase, caffeoyl-CoA O-methyltransferase, cinnamoyl-CoA reductase, and cinnamyl alcohol dehydrogenase were coordinately up-regulated. Provision of increasing levels of exogenously supplied Phe to saturating levels (40 mM) to the induction medium resulted in further up-regulation of their transcript levels in the P. taeda cell cultures; this in turn was accompanied by considerable increases in both p-coumaryl and coniferyl alcohol formation and excretion. By contrast, transcript levels for both cinnamate 4-hydroxylase and p-coumarate 3-hydroxylase were only slightly up-regulated. These data, when considered together with metabolic profiling results and genetic manipulation of various plant species, reveal that carbon allocation to the pathway and its differential distribution into the two monolignols is controlled by Phe supply and differential modulation of cinnamate 4-hydroxylase and p-coumarate 3-hydroxylase activities, respectively. The coordinated up-regulation of phenylalanine ammonia lyase, 4-coumarate:CoA ligase, caffeoyl-CoA O-methyltransferase, cinnamoyl-CoA reductase and cinnamyl alcohol dehydrogenase in the presence of increasing concentrations of Phe also indicates that these steps are not truly ratelimiting, because they are modulated according to metabolic demand. Finally, the transcript profile of a putative acid/ester O-methyltransferase, proposed as an alternative catalyst for O-methylation leading to coniferyl alcohol, was not up-regulated under any of the conditions employed, suggesting that it is not, in fact, involved in monolignol biosynthesis.

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Section: Table I Primers and Probes Used For Cloning And Quantitativesupporting

confidence: 80%

Transcriptional Control of Monolignol Biosynthesis in Pinus taeda

Anterola

Jeon

Davin

et al. 2002

Journal of Biological Chemistry

125

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“…The ACCTA-containing sequence most proximal to the C4H transcription initiation site overlaps the sequence CACGTA, which is similar to the G-box sequence CACGTG, previously identified in other light inducible promoters (Loake et al 1992). In addition, two similar sequence motifs for A-box CCGTTA were found at -148 and -272 from the ATG codon and a wound responsive element WRE3 (CCACCT) as reported by Whitbred and Schuler (2000).…”

Section: Resultssupporting

confidence: 73%

Isolation and molecular characterization of cinnamate 4-hydroxylase from apricot and plum

Pina¹,

Zhebentyayeva²,

Errea³

et al. 2012

Biologia plant.

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Cinnamate 4-hydroxylase (C4H) is the second enzyme in the phenylpropanoid pathway which participates in the synthesis of numerous phenylpropanoid compounds such as flavonoids, lignins, suberins and others. We identified a gene putatively coding for Class I C4H in apricot and plum and we analyzed the expression pattern of this gene under different apricot/plum graft combinations with different degree of compatibility. The full-length cDNA is 1 739 bp with a 1 515 bp open reading frame encoding a protein of 504 amino acids. Like other C4Hs, the predicted C4H polypeptides included conserved domains of cytochrome P 450 . The genomic sequence of the apricot C4H gene was interrupted by two introns 335 bp and 904 bp long. Several regulatory motifs including P-, A-, L-and H-boxes, which were conserved across phenylpropanoid metabolism-related genes in higher plants, were found in a 1 300 bp upstream promoter region of the apricot C4H gene. A phylogenetic analysis showed that all Prunus sequences clustered together and were closely related to Malus and Rubus C4H genes. The transcription of Class I PruC4H was detected in all the examined graft combinations, which suggested its rather constitutive character.

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“…AJ495774) was obtained by RACE experiments starting with TDF 13a-5. P450s are involved in various reactions including biosynthesis of secondary metabolites that can play a role in plant defense (Frey et al, 1997;Whitbred and Schuler, 2000). Another possible function of P450s is the generation of reactive oxygen species (Puntarulo and Cederbaum, 1998).…”

Section: Discussionmentioning

confidence: 99%

Identification of powdery mildew-induced barley genes by cDNA-AFLP: functional assessment of an early expressed MAP kinase

et al. 2004

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Gene expression analysis by cDNA-AFLP in barley ( Hordeum vulgare L.) after powdery mildew ( Blumeria graminis f.sp. hordei , Bgh ) inoculation revealed 615 (3.7%) of 16 500 screened cDNA fragments being differentially regulated 4 and/or 12 h after inoculation. Of these transcript derived fragments (TDFs), 120 were sequenced, and for 28 out of 29 tested, induction was confirmed via RT-PCR. Most TDFs did not show any homology to sequences with known functions, others showed homology to genes involved in primary and secondary metabolism, pathogen response, redox regulation, and signal transduction. TDFs with homology to a MAP kinase ( PWMK1 ), a WRKY transcription factor, a heparanase, an immunophilin, a cytochrome P450, and a receptor-like protein kinase were isolated as full length cDNAs. Knockdown by RNA interference via biolistic delivery of sequence specific double stranded RNA to leaf segments tagged two of these genes as possible candidates being causally involved in the outcome of the barley- Bgh interaction. Knockdown of the receptor-like protein kinase and the WRKY transcription factor increased resistance to the fungus, while knockdown of PWMK1 only led to a slightly enhanced susceptibility of epidermal cells to Bgh . This suggests that the receptor-like protein kinase and the WRKY protein are candidates for negative regulators of powdery mildew resistance. Based on expression analyses, PWMK1 appears to be more generally involved in stress response.

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Molecular Characterization of CYP73A9 andCYP82A1 P450 Genes Involved in Plant Defense in Pea

Cited by 69 publications

References 43 publications

Transcriptional Control of Monolignol Biosynthesis in Pinus taeda

Transcriptional Control of Monolignol Biosynthesis in Pinus taeda

Isolation and molecular characterization of cinnamate 4-hydroxylase from apricot and plum

Identification of powdery mildew-induced barley genes by cDNA-AFLP: functional assessment of an early expressed MAP kinase

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