Molecular characterization of<i>Staphylococcus aureus</i>strains in bovine mastitis milk in Bangladesh

Hoque, M. Nazmul; Das, Ziban Chandra; Rahman, Ashikur; Haider, MG; Islam, Md. Rayhanul

doi:10.1016/j.ijvsm.2018.03.008

Cited by 95 publications

(106 citation statements)

References 34 publications

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“…In bovine mastitis, predominantly identified pathogens are bacteria 6,7,[10][11][12][13][14][15][16] , however, other concomitant microbial players like archaea and viruses 6,12 could also be detected, highlighting the novel insights from metagenomics compared to amplicon sequencing. The bovine milk microbiome was dominated by Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes,…”

Section: Discussionmentioning

confidence: 99%

“…Metagenomics 6,7,[13][14][15] and isolate sequencing 35 of mastitis pathogens provided overwhelming evidence that microbial traits, for instance, pathogenicity, virulence, antibiotic resistance, and metabolic potentials are linked with strain-specific genomic characteristics 21,[36][37][38] .…”

Section: Discussionmentioning

confidence: 99%

“…Following pathogen invasion and subsequent establishment in the mammary gland, either CM or subclinical mastitis (SCM) may present itself. Bovine CM is one of the most frequent diseases (with visibly abnormal milk, and swollen, hot, painful, and reddened udder) affecting the global dairy industry 16,17 , and diverse groups of microbial communities colonizing the mammary gland and/or quarters have evolved novel mechanisms that facilitate their proliferation during the disease process 6 . One of the very frustrating aspects of bovine CM is its recurrent nature, reinfection of a quarter or udder after bacteriological cure 18 .…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, the virulome, the set of genes encoding virulence, can also be carried on the mobilome 9 , and thus, many virulence factors associated genes (VFGs) easily spread in bacterial populations by horizontal gene transfer, converting mutualistic or commensal bacteria into potential opportunistic pathogens 7,21,24 . Though the exact functions of archaea and viruses in the progression of mastitis are not fully explored like bacterial pathogens 6,10,16 , the dynamic shifts in their composition and relative abundances in the onset of CM, persistent or chronic recurrent CM (RCM), invisible udder infections (SCM), and the healthy conditions of the mammary glands and/or quarters could be resulted from a host genetic predisposition to colonization, immune responses to milk bacteria involving increased levels of polymorphonuclear infiltrate, differences mediated by the microbial composition itself, or a combination of these effectors 6,12,24 . However, it's not fully understood what exactly happens to the microbiome when disease states change;…”

Section: Introductionmentioning

confidence: 99%

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Microbiome Dynamics of Bovine Mastitis Progression and Genomic Determinants

Hoque

Istiaq

Rahman

et al. 2020

Preprint

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The milk of lactating cows presents a complex ecosystem of interconnected microbial communities which can impose a significant influence on the pathophysiology of mastitis. Previously, we reported the alteration of microbiome (bacteria, archaea, virus) composition between clinical mastitis (CM) and healthy (H) milk. We hypothesized possible dynamic shifts of microbiome compositions with the progress of different pathological states of mastitis (CM, Recurrent CM; RCM, Subclinical Mastitis; SCM) determined by its favoring genomic potentials. To evaluate this hypothesis, we employed whole metagenome sequencing (WMS) in 20 milk samples (CM = 5, RCM = 6, SCM = 4, H = 5) to unravel the microbiome dynamics, interrelation, and relevant metabolic functions. PathoScope (PS) and MG-RAST (MR) analyses mapped the WMS data to 442 bacterial, 58 archaeal and 48 viral genomes with distinct variation in microbiome composition and abundances across these metagenomes (CM>H>RCM>SCM). PS analysis identified 385, 65, 80 and 144 bacterial strains in CM, RCM, SCM, and H milk, respectively, with an inclusion of 67.19% previously unreported opportunistic strains in mastitis metagenomes. Moreover, MR detected 56, 13, 9 and 46 archaeal, and 40, 24, 11 and 37 viral genera in CM, RCM, SCM and H-milk metagenomes, respectively. The CM-microbiomes had closest association with RCM-microbiomes followed by SCM, and H-microbiomes. Furthermore, we identified 333, 304, 183 and 50 virulence factors-associated genes (VFGs), and 48, 31, 11 and 6 antibiotic resistance genes (AGRs) in CM, RCM, SCM, and H-microbiomes, respectively, showing a significant correlation between the relative abundances of VFGs (p = 0.001), ARGs (p = 0.0001), and associated bacterial taxa. We also detected correlated variations in the presence and abundance of several metabolic functional genes related to bacterial colonization, proliferation, chemotaxis, motility and invasion, oxidative stress, virulence and pathogenicity, phage integration and excision, biofilm-formation, and quorum-sensing to be associated with different episodes of mastitis. Therefore, profiling the dynamics of microbiome in different states of mastitis, concurrent VFGs, ARGs, and genomic functional correlations will contribute to developing microbiome-based diagnostics and therapeutics for bovine mastitis, and carries significant implications on curtailing the economic fallout from this disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microbiome Dynamics of Bovine Mastitis Progression and Genomic Determinants

Hoque

Istiaq

Rahman

et al. 2020

Preprint

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“…Mastitis is a serious problem in dairy industry worldwide which has a profound impact on animal health [1,2]. S. aureus is a major representative pathogenic bacterium causing bovine mastitis [3,4].…”

Section: Introductionmentioning

confidence: 99%

S. Aureus Lipoteichoic Acid Failed to Activate NLRP3, but Downregulated the Transduction of NLRP3/MAPK and NF-κB Signaling Pathway in Mouse Mastitis Induced by S. Aureus

Shen

et al. 2020

Preprint

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Background It was confirmed that S. aureus infection activated the expression of NLRP3. As a major component of S. aureus cell wall, what about LTA? Firstly, in this study a mouse model was built following LTA intramammary infection for 0, 12, 24, 48 and 72 h, the characterization of mammary inflammatory response induced by LTA was observed. Subsequently, mouse mammary preconditioned with LTA was modelling. The effect of LTA treatment on the activation of NLRP3/ MAPK and NF-κB signaling pathways induced by S. aureus were detected.Results Results shown that the LTA intramammary infection induce mild but rapid recovery inflammation in mammary gland whereas failed to stimulate NLRP3. LTA treatment protected mammary gland against S. aureus infection by suppressed the activation of NLRP3/ MAPK and NF-κB signaling pathways. Meanwhile it is noteworthy that the expression of NLRP3/MAPK JNK induced by S. aureus was not regulated by LTA treatment.Conclusions These results suggested LTA induced mammary gland inflammation was mild and self-limiting. LTA treatment revealed a good anti-inflammation effect through downregulating the transduction of NLRP3/MAPK and NF-κB signaling pathways in mouse mastitis induced by S. aureus. Our research highlights the importance of an LTA during the innate immune response of the mammary gland and offer novel insights for new approaches concerning effective immunomodulation against a local bacterial infection.

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Evaluation of the Knowledge, Attitudes and Practice of Veterinary Professionals and Senior Animal Health Students in Central Gondar Zone, Gondar, Ethiopia: Antimicrobial Use and Resistance Perspectives

Mekonen,

Mekasha,

Feleke

et al. 2024

Veterinary Medicine & Sci

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BackgroundAntimicrobial resistance (AMR) poses a worldwide challenge to the well‐being of animals and humans. The emergence of AMR is largely attributed to the improper utilization and overuse of antimicrobials. In order to combat this issue and promote responsible antimicrobial use (AMU), it is imperative for veterinary professionals and graduating students to possess extensive knowledge, maintain positive attitudes, and adhere to good practices regarding AMU and AMR.ObjectiveTo assess the knowledge, attitude and practices (KAPs) of veterinary professionals and senior animal health students’ towards veterinary AMU and AMR.MethodsA cross‐sectional study design was conducted on purposefully selected 200 participants (60 of them were veterinary professionals from the districts and towns, and the remaining 140 were senior animal health university students). A self‐administered, structured questionnaire was used to collect the necessary data, and descriptive statistics were used to summarize the data.ResultsThe study revealed that, overall, study participants’ KAP were not satisfactory. From veterinary professionals, about 56.7% of respondents scored average knowledge, whereas 66.7% and 61.66% of them had negative attitudes and poor practices, respectively, on AMU and AMR. The study found that 56% of the participants’ think the previous knowledge they acquired regarding AMU and AMR was not sufficient, and the internet was used as a source of drug information by the majority of participants. The majority (72%) of them were not taking special training on AMU and AMR. Regarding senior animal health students, this study indicated that 52% and 69.2% of the respondents had poor knowledge and a negative attitude, respectively, towards AMU and AMR.ConclusionOverall, veterinary professionals in the central Gondar zone have average knowledge, a negative attitude and poor practices. Poor knowledge and a negative attitude were also observed among senior animal health students towards AMU and AMR. Therefore, to fill the observed gaps, activities that enhance the skills of veterinary professionals and students should be implemented. Ingreted measures with animicrobial stewardship program and Nationwide studies are highly suggested to evaluate the overall status of veterinary professionals towards AMU and AMR.

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Molecular characterization ofStaphylococcus aureusstrains in bovine mastitis milk in Bangladesh

Cited by 95 publications

References 34 publications

Microbiome Dynamics of Bovine Mastitis Progression and Genomic Determinants

Microbiome Dynamics of Bovine Mastitis Progression and Genomic Determinants

S. Aureus Lipoteichoic Acid Failed to Activate NLRP3, but Downregulated the Transduction of NLRP3/MAPK and NF-κB Signaling Pathway in Mouse Mastitis Induced by S. Aureus

Evaluation of the Knowledge, Attitudes and Practice of Veterinary Professionals and Senior Animal Health Students in Central Gondar Zone, Gondar, Ethiopia: Antimicrobial Use and Resistance Perspectives

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