Molecular characterization of Indian isolate of peanut mottle virus and immunodiagnosis using bacterial expressed core capsid protein

Soumya, K.; Yogita, M.; Prasanthi, Y; Anitha, K.; Kishor, P. B. Kavi; Jain, Rakesh; Mandal, Bikash

doi:10.1007/s13337-014-0210-3

Cited by 10 publications

(2 citation statements)

References 26 publications

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“…The recombinant approach is advantageous as it does not encounter the problems associated with the mixture of closely related viruses, purification and maintenance of live virus culture. This strategy has been successfully used for a number of plant viruses from different genera however not for carmoviruses [4,9,13,16]. To our knowledge, this is the first report of development of immunodiagnosis based on antiserum against bacterial expressed recombinant CP of any carmoviruses in general and SYMMV in particular.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of a new variant of soybean yellow mottle mosaic virus with extended host-range in India

et al. 2015

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Soybean yellow mottle mosaic virus (SYMMV, genus Carmovirus) was previously known to occur in South Korea and USA causing bright yellow mosaic in soybean. In this study, SYMMV (Car-Mb14 isolate) was isolated from mungbean (Vigna radiata) exhibiting mild mottling and puckering symptoms in the experimental field at Indian Agricultural Research Institute, New Delhi during 2012. The virus isolate, Car-Mb14 induced veinal mottling, mild mottling, chlorotic blotching, local and systemic necrosis in soybean, mungbean, blackgram, French bean and guar bean, respectively. The symptomatology of the present isolate of SYMMV was different from the previously reported South Korean isolate, as the later did not induce symptoms in any of the above legumes other than soybean. The present isolate was phylogenetically distinct and shared 90-93 % sequence identity in coat protein (CP) of 52 SYMMV isolates reported from Korea and USA. In order to know the serological relationships, the CP gene of the present isolate was over expressed as a 39 kDa protein in E. coli and an antiserum of 1:16,000 titer against the recombinant CP was produced. Serological cross reactivity analysis revealed that SYMMV was serologically related to blackgram mottle virus but not to cowpea mottle virus, the other legume infecting carmoviruses. The antiserum was used to detect prevalence of SYMMV in legume crops by ELISA. Out of 145 field samples of legumes (mungbean, blackgram, French bean and soybean) collected from different places in India, SYMMV was detected only in 16 samples of mungbean and one sample of blackgram. The natural infection of SYMMV in mungbean and blackgram was further confirmed based on CP gene sequence. This study provides evidence of occurrence of a new variant of SYMMV with distinct symptom phenotype and extended host-range in India.

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Section: Discussionmentioning

confidence: 99%

Diagnosis of a new variant of soybean yellow mottle mosaic virus with extended host-range in India

et al. 2015

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“…Molecular detection and characterization of the plant virus have been achieved through reverse transcriptionpolymerase chain reactions (RT-PCR) using primers specific to a strain or group of the virus (Moreno et al 2004;Adam et al 2005;Massumi et al 2009;Dang et al 2010;Hou et al 2011;Soumya et al 2014). Among several genes in the virus, the CP gene is most often used for virus characterization (Suranto et al 1998;Elmahdi and El Salam 2016;Subiastuti et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Coat protein gene of a PStV-Bm isolate from West Nusa Tenggara, Indonesia

et al. 2020

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Abstract. Julisaniah NI, Suharjono, Mastuti R, Arumingtyas EL. 2020. Coat protein gene of a PStV-Bm isolate from West Nusa Tenggara, Indonesia. Biodiversitas 21: 903-909. Peanut stripe virus (PStV) is a single-stranded positive-sense RNA virus capable of infecting peanut plants. An isolate of PStV (PStV-Bm) was collected from a peanut field in the Bima District, West Nusa Tenggara Province, Indonesia and the coat protein (CP) gene of this virus (CP-PStV) was extracted from the viral RNA and analyzed using reverse transcription-polymerase chain reaction methods. The CP-PStV gene of PStV-Bm was aligned with several PStV genes deposited in the Genbank (http://www.ncbi.nml.nih.gov). Based on the nucleotide sequence of the CP gene, PStV-Bm was grouped into a similar cluster with other PStVs that originated from Indonesia with a similar index, ranging from 96.8% to 98.9%. Genetic similarity (about 96.1%) was also observed between PStV-Bm and PStV from the USA. This genetic similarity indicated that viruses from adjacent regions have high genetic relationships. Some amino acid differences were observed in PStV-Bm that may be typical of this strain.

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Application of Immuno-Diagnosis for Plant Viruses Occurring in India

Bhat

Maheshwari

2017

A Century of Plant Virology in India

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Molecular characterization of Indian isolate of peanut mottle virus and immunodiagnosis using bacterial expressed core capsid protein

Cited by 10 publications

References 26 publications

Diagnosis of a new variant of soybean yellow mottle mosaic virus with extended host-range in India

Diagnosis of a new variant of soybean yellow mottle mosaic virus with extended host-range in India

Coat protein gene of a PStV-Bm isolate from West Nusa Tenggara, Indonesia

Application of Immuno-Diagnosis for Plant Viruses Occurring in India

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