Molecular characterization of KPC-2-producing Klebsiella pneumoniae ST258 isolated from bovine mastitis

Silva-Sánchez, Jesús; Barrios-Camacho, Humberto; Hernández-Rodriguez, Emmanuel; Dúran-Bedolla, Josefina; Sánchez-Pérez, Alejandro; Martínez-Chavarría, Luary C.; Xicohtencatl‐Cortes, Juan; Hernández‐Castro, Rigoberto; Garza–Ramos, Ulises

doi:10.1007/s42770-021-00445-y

Cited by 14 publications

(6 citation statements)

References 51 publications

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“…The rapid emergence of carbapenem-resistant bacterial isolates in livestock, food-producing animals, and wildlife is currently a significant public health concern that further muddies the picture of antibiotic resistance worldwide [15]. It is generally known that K. pneumoniae has become resistant to carbapenems [40].…”

Section: Plos Onementioning

confidence: 99%

“…Several countries, including India [11], Italy [12], Japan [13], and the United Kingdom [14], have documented the existence of extended spectrum beta-lactamase resistance related multidrug resistant (MDR) (ESBL-MDR) K. pneumoniae isolates. However, the emergence of carbapenemase producing K. pneumoniae responsible for mastitis has been reported somewhere else [15]. Although, limited data with diverse genetic information of heterogeneous nature among bovine mastitis associated nutritional analysis and K. pneumoniae strains remain the topic of debate.…”

Section: Introductionmentioning

confidence: 99%

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Nutritional analysis and characterization of carbapenemase producing-Klebsiella pneumoniae resistant genes associated with bovine mastitis infected cow’s milk

Mr. Saddam,

Khan,

Jamal

et al. 2023

PLoS ONE

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The current study was designed to analyze nutritional parameters and to characterize carbapenemase producing-Klebsiella pneumoniae isolates from bovine mastitic cow’s milk. Out of 700 milk samples K. pneumoniae was identified by phenotypic and molecular techniques along with their antibiogram analysis and nutritional analysis was performed using the procedure of Association of Official Analytical Chemists. Carbapenemase-producing K. pneumoniae was detected by phenotypic CarbaNP test followed by molecular characterization of their associated resistant genes blaVIM, blaKPC, blaOXA-48, blaNDM, and blaIMP along with insertion sequence common region 1 (ISCR1) and integrons (Int1, Int2, and Int3) genes. Among nutritional parameters, fat content was observed (2.99%) followed by protein (2.78%), lactose (4.32%), and total solid (11.34%), respectively. The prevalence of K. pneumoniae among bovine mastitis was found 25.71%. Antibiogram analysis revealed that more effective antibiotics was ceftazidime (80%) followed by amikacin (72%), while highly resistant antibiotics was Fusidic acid (100%). Distribution of carbapenemase producer K. pneumoniae was found 44.4%. Among carbapenem resistant genes blaKPC was found 11.25%, blaVIM 2.75%, blaNDM 17.5%, and blaOXA-48 7.5%, while blaIMP gene was not detected. Furthermore, distribution of ISCR1 was found 40%, while integron 1 was found 61.2% followed by integron 2 (20%), and integron 3 (5%). In conclusion, the recent scenario of carbapenemase resistant K. pneumoniae isolates responsible for mastitis may affect not only the current treatment regime but also possess a serious threat to public health due to its food borne transmission and zoonotic potential.

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Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nutritional analysis and characterization of carbapenemase producing-Klebsiella pneumoniae resistant genes associated with bovine mastitis infected cow’s milk

Mr. Saddam,

Khan,

Jamal

et al. 2023

PLoS ONE

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“…In general, K. pneumoniae lineages from humans and non-human niches share limited genetic overlap [36], and no specific lineage has become dominant among ones causing mastitis in dairy cows [36]. Nevertheless, KPC-2-positive ST258 strains were recently detected among dairy cows with clinical mastitis in Mexico at an alarming rate [37]. K. pneumoniae from mastitis appears to have a low prevalence of ESBLs.…”

Section: Antimicrobial-resistant Gram-negative Pathogens From Bovine ...mentioning

confidence: 99%

“…Therefore, the risk of transmission associated with the consumption of raw milk will be dependent on the prevalence of these organisms in raw milk samples as well as on the ability of these strains to persist in the intestinal environment of humans following the consumption of contaminated milk. In that regard, the mere presence of specific human strains such as K. pneumoniae ST258 in milk samples is certainly of concern and should be further explored [37,55,56].…”

Section: Transmissionmentioning

confidence: 99%

Extended-Spectrum Beta-Lactamase-Producing Escherichia coli and Other Antimicrobial-Resistant Gram-Negative Pathogens Isolated from Bovine Mastitis: A One Health Perspective

Nery Garcia,

Dantas,

da Silva Barbosa

et al. 2024

Antibiotics

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Antimicrobial resistance (AMR) poses an imminent threat to global public health, driven in part by the widespread use of antimicrobials in both humans and animals. Within the dairy cattle industry, Gram-negative coliforms such as Escherichia coli and Klebsiella pneumoniae stand out as major causative agents of clinical mastitis. These same bacterial species are frequently associated with severe infections in humans, including bloodstream and urinary tract infections, and contribute significantly to the alarming surge in antimicrobial-resistant bacterial infections worldwide. Additionally, mastitis-causing coliforms often carry AMR genes akin to those found in hospital-acquired strains, notably the extended-spectrum beta-lactamase genes. This raises concerns regarding the potential transmission of resistant bacteria and AMR from mastitis cases in dairy cattle to humans. In this narrative review, we explore the distinctive characteristics of antimicrobial-resistant E. coli and Klebsiella spp. strains implicated in clinical mastitis and human infections. We focus on the molecular mechanisms underlying AMR in these bacterial populations and critically evaluate the potential for interspecies transmission. Despite some degree of similarity observed in sequence types and mobile genetic elements between strains found in humans and cows, the existing literature does not provide conclusive evidence to assert that coliforms responsible for mastitis in cows pose a direct threat to human health. Finally, we also scrutinize the existing literature, identifying gaps and limitations, and propose avenues for future research to address these pressing challenges comprehensively.

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“…Six of the 12 tested K. pneumoniae representatives of different pulsotypes belonged to IncA/C. KPC-2-producing K. pneumoniae isolates from bovine mastitis in Mexico were characterized using MLST, which showed that all isolates comprised two clones belonging to ST258 [47] . Phylogenetic analysis of K. pneumoniae ST340 strains using MLST revealed the presence and distribution of bla NDM-5 in porcine pneumonia isolates from China [48] .…”

Section: Molecular Typing Of K Pneumoniaementioning

confidence: 99%

Molecular typing methods & resistance mechanisms of MDR <i>Klebsiella pneumoniae</i>

2023

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<abstract> <p>The emergence and transmission of carbapenem-resistant <italic>Klebsiella pneumoniae</italic> (CRKP) have been recognized as a major public health concern. Here, we investigated the molecular epidemiology and its correlation with the mechanisms of resistance in CRKP isolates by compiling studies on the molecular epidemiology of CRKP strains worldwide. CRKP is increasing worldwide, with poorly characterized epidemiology in many parts of the world. Biofilm formation, high efflux pump gene expression, elevated rates of resistance, and the presence of different virulence factors in various clones of <italic>K. pneumoniae</italic> strains are important health concerns in clinical settings. A wide range of techniques has been implemented to study the global epidemiology of CRKP, such as conjugation assays, 16S-23S rDNA, string tests, capsular genotyping, multilocus sequence typing, whole-genome sequencing-based surveys, sequence-based PCR, and pulsed-field gel electrophoresis. There is an urgent need to conduct global epidemiological studies on multidrug-resistant infections of <italic>K. pneumoniae</italic> across all healthcare institutions worldwide to develop infection prevention and control strategies. In this review, we discuss different typing methods and resistance mechanisms to explore the epidemiology of <italic>K. pneumoniae</italic> pertaining to human infections.</p> </abstract>

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Molecular characterization of KPC-2-producing Klebsiella pneumoniae ST258 isolated from bovine mastitis

Cited by 14 publications

References 51 publications

Nutritional analysis and characterization of carbapenemase producing-Klebsiella pneumoniae resistant genes associated with bovine mastitis infected cow’s milk

Nutritional analysis and characterization of carbapenemase producing-Klebsiella pneumoniae resistant genes associated with bovine mastitis infected cow’s milk

Extended-Spectrum Beta-Lactamase-Producing Escherichia coli and Other Antimicrobial-Resistant Gram-Negative Pathogens Isolated from Bovine Mastitis: A One Health Perspective

Molecular typing methods & resistance mechanisms of MDR <i>Klebsiella pneumoniae</i>

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Molecular characterization of KPC-2-producing Klebsiella pneumoniae ST258 isolated from bovine mastitis

Cited by 14 publications

References 51 publications

Nutritional analysis and characterization of carbapenemase producing-Klebsiella pneumoniae resistant genes associated with bovine mastitis infected cow’s milk

Nutritional analysis and characterization of carbapenemase producing-Klebsiella pneumoniae resistant genes associated with bovine mastitis infected cow’s milk

Extended-Spectrum Beta-Lactamase-Producing Escherichia coli and Other Antimicrobial-Resistant Gram-Negative Pathogens Isolated from Bovine Mastitis: A One Health Perspective

Molecular typing methods &amp; resistance mechanisms of MDR <i>Klebsiella pneumoniae</i>

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Molecular typing methods & resistance mechanisms of MDR <i>Klebsiella pneumoniae</i>