Molecular characterization of &lt;i&gt;Fasciola gigantica&lt;/i&gt; in Delhi, India and its phylogenetic relation to the species from South Asian countries

Mohanta, Uday Kumar; Neeraja, Tambireddy; Itagaki, Tadashi

doi:10.1292/jvms.16-0126

Cited by 15 publications

(2 citation statements)

References 20 publications

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“…In some countries, F. gigantica is the dominant species in livestock. For example, in Delhi, India, all the 40 Fasciola isolates from 11 buffalos belonged to F. gigantica (Hayashi, Mohanta, et al., 2016 ). Moreover, 96.9% of Fasciola isolates from cattle and buffalo in Nghe An province, Vietnam, belonged to F. gigantica (Anh et al., 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran

Sharbatkhori

Nasibi

Mohammadi

et al. 2023

Veterinary Medicine & Sci

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Background: Fascioliasis, caused by the liver flukes Fasciola hepatica and Fasciola gigantica, is a global zoonotic helminthic disease. The livestock and human are the final hosts of the parasites. Northern Iran is an important endemic region for fascioliasis. Few studies have been conducted on the characterization of Fasciola isolates from eastern regions of the Caspian littoral of the country. Objective:The aim of the present study was to identify F. hepatica, F. gigantica and intermediate/hybrid forms of Fasciola isolates from livestock in Golestan province, northern Iran, using morphometric and molecular tools.Methods: Livestock livers naturally infected with Fasciola spp. were collected from Golestan slaughterhouse during 2019-2020. The worms were morphometrically studied using a calibrated stereomicroscope. Genomic DNA was extracted from all samples, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed on internal transcribed spacer (ITS1) region using Rsa1 restriction enzyme. All the isolates were then analysed by multiplex PCR on Pepck region.Results: A total of 110 Fasciola isolates were collected from the infected livers, including 94 sheep, 12 cattle and 4 goats. Morphometric analysis of 61 adult Fasciola isolates indicated that, 44 and 17 isolates belonged to F. hepatica and F. gigantica, respectively.Eighty-one and 29 isolates belonged to F. hepatica and F. gigantica using ITS1-RFLP, respectively. However, Pepck Multiplex PCR indicated 72 F. hepatica, 26 F. gigantica and 12 intermediate/hybrid forms. All 12 hybrid isolates were found in sheep host. Two isolates were identified as F. gigantica using morphometry and F. hepatica using both molecular methods. Conclusion:The present study confirmed the existence of both F. hepatica and F. gigantica species and reported the first molecular evidence of hybrid Fasciola isolates in ruminants of Golestan province.

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Section: Discussionmentioning

confidence: 99%

Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran

Sharbatkhori

Nasibi

Mohammadi

et al. 2023

Veterinary Medicine & Sci

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“…Fascioliasis is also the most widespread parasitic disease of ruminants worldwide. Furthermore, it affects humans in 51 countries across several continents, including up to 17 million people, and more than 180 million people are at risk worldwide [1,2], thereby causing important economic losses to sheep and cattle in commercial herds [3,4]. Cattle infections usually occur by the ingestion of aquatic plants that contain the infective metacercariae [5].…”

Section: Introductionmentioning

confidence: 99%

Immunodiagnosis of cattle fascioliasis using a 27 kDa Fasciola gigantica antigen

et al. 2021

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Background and Aim: Diagnosis of fascioliasis depends on clinical symptoms and routine laboratory tests. Recently, antibodies and circulating antigens of Fasciola were used for detecting active infections. Therefore, this study aimed to identify Fasciola gigantica antigens in the sera of infected cattle using Western blotting and enzyme-linked immunosorbent assay (ELISA) for an accurate diagnosis of cattle infected with F. gigantica. Materials and Methods: Serum samples were obtained from 108, 23, and 19 cattle infected with Fasciola gigantica, Paramphistomum cervi, and Strongylids, respectively, including 57 non-infected cattle that were used as healthy cattle for the study. Western blotting and ELISA were then used to detect circulating Fasciola antigens at 27 kDa. Results: The target epitope was detected in an F. gigantica adult-worm antigen preparation, excretory/secretory products, and serum from cattle infected with F. gigantica. However, it was absent in sera from P. cervi, Strongylids, and healthy cattle. The purified 27 kDa F. gigantica (FPA-27) antigen was also detected in cattle serum using ELISA with high degrees of sensitivity and specificity (94% and 82%, respectively), and the area under the receiver operating characteristic curve was 0.89 with a highly significant correlation of p<0.0001. Conclusion: The FPA-27 is proposed to be a promising candidate for the serodiagnosis of fascioliasis in cattle.

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Fasciolosis

Singh¹,

Singh²,

Singh³

et al. 2021

Fasciolosis: Causes, Challenges and Controls

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Molecular characterization of <i>Fasciola gigantica</i> in Delhi, India and its phylogenetic relation to the species from South Asian countries

Cited by 15 publications

References 20 publications

Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran

Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran

Immunodiagnosis of cattle fascioliasis using a 27 kDa Fasciola gigantica antigen

Fasciolosis

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