Molecular Characterization of MaCCS, a Novel Copper Chaperone Gene Involved in Abiotic and Hormonal Stress Responses in Musa acuminata cv. Tianbaojiao

Abstract

Copper/zinc superoxide dismutases (Cu/ZnSODs) play important roles in improving banana resistance to adverse conditions, but their activities depend on the copper chaperone for superoxide dismutase (CCS) delivering copper to them. However, little is known about CCS in monocots and under stress conditions. Here, a novel CCS gene (MaCCS) was obtained from a banana using reverse transcription PCR and rapid-amplification of cDNA ends (RACE) PCR. Sequence analyses showed that MaCCS has typical CCS domains and a conserved gene structure like other plant CCSs. Alternative transcription start sites (ATSSs) and alternative polyadenylation contribute to the mRNA diversity of MaCCS. ATSSs in MaCCS resulted in one open reading frame containing two in-frame start codons to form two protein versions, which is supported by the MaCCS subcellular localization of in both cytosol and chloroplasts. Furthermore, MaCCS promoter was found to contain many cis-elements associated with abiotic and hormonal responses. Quantitative real-time PCR analysis showed that MaCCS was expressed in all tested tissues (leaves, pseudostems and roots). In addition, MaCCS expression was significantly induced by light, heat, drought, abscisic acid and indole-3-acetic acid, but inhibited by relatively high concentrations of CuSO4 and under cold treatment, which suggests that MaCCS is involved in abiotic and hormonal responses.

In silico identification and expression analysis of superoxide dismutase (SOD) gene family in Medicago truncatula

“…Two specific conserved metal-binding motifs, MXCXXC and CXC, were found to be present in MtCSD2 as well as in CsCSD3, MaCCS, and CuZn-SOD-CCh ( Fig. 2b), suggesting that these SODs are homologous to copper chaperones for SOD, which is in agreement with previous reports (Feng et al 2016b;Nath et al 2014;Zhou et al 2017). In addition, the protein motif distribution and gene structures also reveal that the SOD proteins have strong evolutionary conservation.…”

Genome-Wide Identification and Transcriptional Expression Analysis of Cucumber Superoxide Dismutase (SOD) Family in Response to Various Abiotic Stresses

“…CsCSD3 was predicted to be located in extracellular space, suggesting that it participates in the secretory pathway. A blastp search in the NCBI database showed that CsCSD3 was 74.0% and 70.1% identical to copper chaperone for superoxide dismutase (CCS) proteins from rice (CuZn-SOD-CCh) [ 38 ] and banana (MaCCS) [ 39 ], respectively, implying that it belongs to the CCS subfamily. A conserved metal-binding motif (MxCxxC) and another conserved metal-binding motif (CxC) were near the N-terminus and in the C-terminus, respectively ( Figure 2() ).…”

Cold stress‐induced miRNA and degradome changes in four soybean varieties differing in chilling resistance

“…ROS are almost an inseparable factor of abiotic stress in plants; therefore, the capacity of any given organism to neutralize these pernicious molecules is essential for endurance of adverse conditions (Dar et al, 2017). Nature's response to ROS is the development of the antioxidative system, including copper/zinc SOD (Feng et al, 2016). Our data showed downregulation of miR398 in trifoliates of Augusta and G. soja , and the opposite expression pattern was observed in Fiskeby V and Toyomusume.…”