Molecular characterization of mandarin (Citrus reticulata Blanco) using ISSR markers

Abstract

In order to establish the molecular diversity of citrus production systems from Villa Sucre (Colombia), mainly "Creole mandarin", the study analyzed C. reticulata farm’s samples using six ISSR molecular markers. A total of 61 polymorphisms were characterized, 42% of them were highly common, with a mid- average polymorphic information content (PIC) of 0.42 indicating a high polymorphic variation. Molecular relations based in Dice coefficient and the UPGMA algorithm, showed the existing genetic relationships between the crop areas, grouping them in five separate clades and two main subgroups (MDS). This is the first molecular classification done in the area, sets the basis for mandarin crop molecular diversity, and provides vital information for mandarin crop management programs.

GENETIC DIFFERENTIATION OF CULTIVATED CITRUS FRUITS (Citrus spp.) IN COLOMBIA USING SSR MOLECULAR MARKERS

“…These populations had a disimilarity index of 0.144. The mandarins Oneco and Clemenules were located in group III with a dissimilarity of 0.261, and they were clearly differentiated from Ica Bolo and Arrayana, which were associated in group V. Citrus reticulata exhibited high genetic variability, as determined by morphological characters and molecular markers (Froelicher et al, 2008;Tripolitsiotis et al, 2013;Suárez-Contreras et al, 2020). Star Ruby grapefruit (C. paradisi Macf.)…”