Molecular Characterization of Mediterranean Spotted Fever Rickettsia Isolated From a European Traveler in the State of São Paulo, Brazil

Gehrke, Flávia de Sousa; Angerami, Rodrigo Nogueira; Marrelli, Mauro Toledo; Souza, Eliana Rodrigues de; Nascimento, Elvira Maria Mendes do; Colombo, Silvia; Silva, Luiz J. da; Schumaker, Teresinha Tizu Sato

doi:10.1111/j.1708-8305.2012.00677.x

Cited by 4 publications

(5 citation statements)

References 11 publications

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“…Interestingly, we have previously reported an imported and fatal case of Mediterranean spotted fever in a Portuguese traveler that arrived at Brazil, which was also confirmed by rickettsial isolation and molecular detection. 13 The recognition that eschar ( tache noire ) as an important clue for diagnosis of some rickettsial diseases was essential for the suspicion of the case presented here. Because of the cross-reactivity of antibody responses to spotted fever group Rickettsia species, 1 clinicians must be aware of the importance of submitting appropriate samples for molecular detection or isolation techniques when available, as serology alone is not sufficient to identify the species responsible for infection.…”

Section: Discussionmentioning

confidence: 84%

First report of African tick-bite fever in a South American traveler

Angerami

Krawczak

Nieri-Bastos

et al. 2018

SAGE Open Medical Case Reports

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We report a clinical case of African tick-bite fever in a Brazilian traveler right after his return from South Africa. Definitive diagnosis was supported by seroconversion between acute-phase and convalescent-phase serum samples, detection of rickettsial DNA in skin lesions, and in vitro culture of Rickettsia africae from the patient’s skin. Most of the previous reported cases of African tick-bite fever were confirmed solely by serological or/and molecular methods. Through this first confirmed case of African tick-bite fever in Brazil, it is quite possible that other cases are occurring unnoticed by the health authorities, requiring a greater vigilance in traveler’s medicine in South America.

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Section: Discussionmentioning

confidence: 84%

First report of African tick-bite fever in a South American traveler

Angerami

Krawczak

Nieri-Bastos

et al. 2018

SAGE Open Medical Case Reports

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“…The case definition of confirmed SFG includes both clinical evidence and laboratory confirmation. The clinical features of this rickettsiosis consist of an acute undifferentiated febrile illness, often accompanied by headache, myalgia and nauseas, and a maculopapular or vesicular rash may be observed a few days after the onset of illness [13][14][15]. When a patient develops a febrile illness, the suspicion for tick-borne illness should be high in those who live or travel to endemic areas.…”

Section: Rickettsioses Caused By the Genus Rickettsia And Their Diagn...mentioning

confidence: 99%

“…The greatest challenge to clinicians is not therapy but the difficult diagnosis during the early phase of infections [18]. The diagnosis of rickettsiosis can be missed because of these nonspecific initial clinical presentations and the absence of specific laboratory confirmation [22]. Serological diagnosis is usually retrospective; antibody increase takes 15-26 days, thus limiting the clinical impact of diagnosis [23].…”

Section: Rickettsioses Caused By the Genus Rickettsia And Their Diagn...mentioning

confidence: 99%

Current and Future Trends in the Clinical Diagnosis of Rickettsioses Sensu Lato

Missailidis

Godoy²,

Mares-Guia³

et al. 2014

IJTDH

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Aims:The aim of this review is to present Rickettsioses sensu lato, with emphasis on their current and future clinical diagnosis. The review presents the conditions, the agents that cause them, and the current gold standards on their diagnosis in national and international reference centres. Additionally, this review covers the various emerging technologies available in the diagnosis of Rickettsioses and discusses their potential for future use as gold standards in the diagnosis of these diseases. Introduction: The introduction presents Rickettsioses sensu lato and gives a broad overview of the conditions they cause, the issues associated with their current diagnosis and the need for their improved, earlier and more accurate diagnosis, in order to prevent current issues with false negatives, misdiagnosis or delay in the diagnosis associated with these conditions, which often renders them grave or lethal. Main Body: The main body of the review presents in independent sections Rickettsias, Ehrlichia, Anaplasma, Bartonella and Coxiella and the conditions associated with each of these bacteria. Spotted fever, endemic typhus, human granulocytic anaplasmosis, human monocytic ehrlichioses, bartonellosis and Q-fever are some of the conditions associated with this group of proteobacteria. The emphasis is on the clinical diagnosis of these conditions and an overview of the current practice, gold standards in reference

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“…The greatest challenge to clinicians is not therapy but the difficult diagnosis during the early phase of infections [37]. The diagnosis of TBD may be masked due to these initial non-specific clinical presentations and the absence of confirmation by specific laboratory testing [38]. In addition, serological diagnosis is usually retrospective; antibody increase takes 15-26 days, thus limiting the clinical impact of diagnosis [39].…”

Section: Introductionmentioning

confidence: 99%

“…The diagnostic test for BSF and ehrlichiosis recommended in Brazil is a dual test for LD [42,46], even though the condition has different immunological and epidemiological aspects than LD. Thus, the analysis of the second stage of the test, western blot, needs a certain number of bands present to be considered positive [38,44]. However, even following this strict criterion, a larger study conducted in Brazil demonstrated a significant number of false positives (16%) [46].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in the Immunologic Method Applied to Tick-Borne Diseases in Brazil

De-Simone

Chino

2022

Pathogens

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Zoonotic-origin infectious diseases are one of the major concerns of human and veterinary health systems. Ticks, as vectors of several zoonotic diseases, are ranked second only to mosquitoes as vectors. Many ticks’ transmitted infections are still endemic in the Americas, Europe, and Africa and represent approximately 17% of their infectious diseases population. Although our scientific capacity to identify and diagnose diseases is increasing, it remains a challenge in the case of tick-borne conditions. For example, in 2017, 160 cases of the Brazilian Spotted Fever (BSF, a tick-borne illness) were confirmed, alarming the notifiable diseases information system. Conversely, Brazilian borreliosis and ehrlichiosis do not require notification. Still, an increasing number of cases in humans and dogs have been reported in southeast and northeastern Brazil. Immunological methods applied to human and dog tick-borne diseases (TBD) show low sensitivity and specificity, cross-reactions, and false IgM positivity. Thus, the diagnosis and management of TBD are hampered by the personal tools and indirect markers used. Therefore, specific and rapid methods urgently need to be developed to diagnose the various types of tick-borne bacterial diseases. This review presents a brief historical perspective on the evolution of serological assays and recent advances in diagnostic tests for TBD (ehrlichiosis, BSF, and borreliosis) in humans and dogs, mainly applied in Brazil. Additionally, this review covers the emerging technologies available in diagnosing TBD, including biosensors, and discusses their potential for future use as gold standards in diagnosing these diseases.

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Molecular Characterization of Mediterranean Spotted Fever Rickettsia Isolated From a European Traveler in the State of São Paulo, Brazil

Cited by 4 publications

References 11 publications

First report of African tick-bite fever in a South American traveler

First report of African tick-bite fever in a South American traveler

Current and Future Trends in the Clinical Diagnosis of Rickettsioses Sensu Lato

Recent Advances in the Immunologic Method Applied to Tick-Borne Diseases in Brazil

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