Molecular characterization of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from King Abdulaziz Specialist Hospital at Taif City, Saudi Arabia

Lagha, Rihab; Abdallah, Fethi Ben; ALKhammash, Asmaa A.H.; Amor, Nabil; Hassan, Mohamed M.; Mabrouk, Imed; Alhomrani, Majid; Gaber, Ahmed

doi:10.1016/j.jiph.2020.12.001

Cited by 16 publications

(12 citation statements)

References 39 publications

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“…The majority of the isolates were found to be from wounds, aspirate urine, and catheter tips that were mostly collected from male patients. These findings are similar to those of other researchers [ 41 , 42 , 43 ]. Most of the isolates in this study were from hospitalised, immunocompromised patients, which shows the role this opportunistic pathogen plays in HAIs, a view also expressed by others [ 42 , 44 , 45 ].…”

Section: Discussionsupporting

confidence: 93%

“…These findings are similar to those of other researchers [ 41 , 42 , 43 ]. Most of the isolates in this study were from hospitalised, immunocompromised patients, which shows the role this opportunistic pathogen plays in HAIs, a view also expressed by others [ 42 , 44 , 45 ].…”

Section: Discussionsupporting

confidence: 93%

“…These results further highlight the global public health threat resulting from MDR bacterial isolates. Similar but varying findings had been reported by others in the region of this investigation [ 42 , 43 ] as well as in other regions of the world [ 46 , 47 ]. The majority of the isolates in this investigation were ESBL (ESBL-KP) strains, a reasonable number of which were carbapenem-resistant Enterobacteriaceae (CRE), and there was 100% resistance to amoxicillin.…”

Section: Discussionsupporting

confidence: 91%

“…However, the least common resistance in this study was found to be against carbapenems [ 48 ], aminoglycosides [ 51 ], and quinolones [ 50 ]. The isolates in this study were highly susceptible to carbapenems and tigecycline, both of which had also been previously reported [ 42 ] as favorable in the management of K. pneumoniae infections. Generally, the management of such infections is reported to rely on colistin and tigecycline, as resistance to these antibiotics are rare upon the initial isolation of the bacterium [ 52 ].…”

Section: Discussionsupporting

confidence: 80%

“…The isolates in this investigation were also highly resistant to ampicillin/sulbactam (AMS), amoxicillin/clavulanic acid (Aug), piperacillin/tazobactam (Ptz), ceftazidime (Caz), cefepime (Pime), cephalotin (Kf), cefoxitin (Ctt), ceftriaxone (Cro), and cefuroxime (Cxm). These antibiotics have also been reported to be amongst the least effective against K. pneumoniae isolates [ 25 , 42 ]. In Saudi Arabia, highly resistant profiles to penicillins [ 43 , 50 ], cephalosporins, carbapenems, and nitrofurantoin have been described.…”

Section: Discussionmentioning

confidence: 99%

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Genetic Analysis, Population Structure, and Characterisation of Multidrug-Resistant Klebsiella pneumoniae from the Al-Hofuf Region of Saudi Arabia

et al. 2021

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Multidrug-resistant Klebsiella pneumoniae (MDR-KP) is a major public health problem that is globally associated with disease outbreaks and high mortality rates. As the world seeks solutions to such pathogens, global and regional surveillance is required. The aim of the present study was to examine the antimicrobial susceptibility pattern and clonal relatedness of Klebsiella pneumoniae isolates collected for a period of three years through pulse field gel electrophoresis (PFGE). Isolate IDs, antimicrobial assays, ESBL-production, and minimum inhibitory concentrations (MICs) were examined with the Vitek 2 Compact Automated System. IDs were confirmed by 16S rRNA gene sequencing, with the resulting sequences being deposited in NCBI databases. DNA was extracted and resistance genes were detected by PCR amplification with appropriate primers. Isolates were extensive (31%) and multidrug-resistant (65%). Pulsotype clusters grouped the isolates into 22 band profiles that showed no specific pattern with phenotypes. Of the isolates, 98% were ESBL-KP, 69% were carbapenem-resistant Enterobacteriaceae (CRE) strains, and 72.5% comprised the carriage of two MBLs (SIM and IMP). Integrons (ISAba1, ISAba2, and IS18) were detected in 69% of the MDR-KP. Additionally, OXA-23 was detected in 67% of the isolates. This study therefore demonstrates clonal diversity among clinical K. pneumoniae, confirming that this bacterium has access to an enormous pool of genes that confer high resistance-developing potential.

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