Molecular Characterization of Mycobiota and Aflatoxin Contamination of Retail Wheat Flours from Jeddah Markets

Gashgari, Rukaia; Shebany, Yassmin M.; Gherbawy, Youssuf A.

doi:10.1089/fpd.2009.0506

Cited by 26 publications

(23 citation statements)

References 71 publications

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“…The RAPD technique has been used to detect genetic variability between isolates of A. flavus and related species (Batista et al, 2008;Gehlot et al, 2011;Irshad and Nawab, 2012) and to discriminate between aflatoxigenic and non-aflatoxigenic isolates of A. flavus (Lourenço et al, 2007;Gashgari et al, 2010;Sepahvand et al, 2011). The ISSR technique has been employed to investigate the diversity and population structure of A. flavus (Tran-Dinh and Carter, 2000;Batista et al, 2008;Tran-Dinh et al, 2009;Hadrich et al, 2010;Wang et al, 2012) and to determine the similarities and dissimilarities between aflatoxigenic and non-aflatoxigenic isolates of this species (Tran-Dinh et al, 2009;Hatti et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of aflatoxigenic and non-aflatoxigenic Aspergillus flavus isolates collected from corn grains

Mahmoud

Ali²,

El-Aziz³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Twelve species from six fungal genera were found to be associated with corn (Zea mays L.) grain samples collected from three main regions of Saudi Arabia. The average frequencies of the most common genera were Aspergillus (11.4%), Fusarium (9.5%), Penicillium (5.1%), and Alternaria (5.8%). Fifteen isolates of Aspergillus flavus were screened by HPLC for their ability to produce aflatoxins (AF). The percentage of aflatoxigenic A. flavus isolates was 53%. Eight isolates produced AF, at concentrations ranging 0.7-2.9 ppb. Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) molecular markers were used to genetically characterize isolates of A. flavus and to discriminate between the aflatoxigenic and non-aflatoxigenic isolates. RAPD and ISSR analysis revealed a high level of genetic diversity in the A. flavus population, which was useful for genetic characterization. The clustering in the RAPD and ISSR dendrograms obtained was unrelated to geographic origin. The RAPD and ISSR markers could not discriminate between aflatoxigenic and non-aflatoxigenic isolates, but the ISSR primers were somewhat better.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of aflatoxigenic and non-aflatoxigenic Aspergillus flavus isolates collected from corn grains

Mahmoud

Ali²,

El-Aziz³

et al. 2014

Genet. Mol. Res.

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“…Other studies indicated no relationship between RAPD profi les and severity of toxicity (35)(36)(37). In contrast, identical RAPD patterns were demonstrated in patulinproducing Paecilomyces variotii strains (38), ochratoxin A-producing Aspergillus species (39), and aflatoxins-producing A. flavus strains (40). We concluded that RAPD analysis can be used in the intraspecifi c differentiation of toxigenic P. citrinum strains obtained from air samples.…”

Section: Discussionmentioning

confidence: 69%

Genetic Variability of Citrinin-Producing Penicillium Citrinum Strains as Occupational Health Hazards in Northern Iran / Genska Varijabilnost Citrinin-Toksinogenih Sojeva Penicillium Citrinum Kao Izvor Opasnosti Za Zdravlje Profesionalno Izloženih Populacija U Sjevernom Iranu

Khosravi

Sheikhkarami

Shokri

et al. 2012

Archives of Industrial Hygiene and Toxicology

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We evaluated the ability of randomly amplifi ed polymorphic DNA (RAPD) to type citrinin-producing Penicillium citrinum (P. citrinum) strains recovered from the forest's air in northern Iran. A total of 12 P. citrinum strains (P1-P12) were characterised by citrinin production and random amplifi cation of polymorphic DNA (RAPD) technique. All the strains produced citrinin with levels ranging from 1.5 μg mL -1 to 39.6 μg mL -1 (average value: 12.68 μg mL -1 ). Of 11 primers tested, eight primers produced polymorphic amplifi cation patterns. These primers generated a total of 105 reproducible RAPD bands, averaging to 13.1 bands per primer. Dendrogram for each primer indicating the distance of the strains to each other was constructed. RAPD results showed that the collected strains constituted four different clusters. The fi rst cluster included two isolates (P1 and P3). The second cluster included seven isolates (P2, P4, P5, P6, P7, P8, and P10). The third and fourth clusters included one isolate (P9) and two isolates (P11 and P12), respectively. We concluded that RAPD analysis might be used in providing genotypic characters for toxigenic P. citrinum strains typing in epidemiological investigations and public health related risk assessment. Khorsavi AR, et al. CITRININ-PRODUCING PENICILLIUM CITRINUM AND HEALTH HAZARD KEY WORDS: airborne fungi, genetic variability, intraspecifi c differentiation, mycotoxin, random amplifi cation of polymorphic DNA (RAPD)

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“…The mycological profile of wheat grains includes different genera. The most common genera are Aspergillus, Penicillium, Fusarium, and Alternaria isolated from wheat grain in Saudi Arabia (Al-Hazmi, 2010;Gashgari et al, 2010). The contamination of cereal grains by fungi is a worldwide problem, where there are several reports documenting the presence of these fungi in samples from the United States (Cleveland et al, 2003), Argentina (Roige et al, 2009), Spain (Sardiñas et al, 2011), and Algeria (Riba et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…It was recognized that different strains of A. flavus vary tremendously in their ability to produce AF. These results showed that total AF occurrence in wheat flour in Jeddah (Saudi Arabia) could not be a relatively critical point, regarding quality of wheat flour (Gashgari et al, 2010). Several authors have used a selective analytical method for the determination of AFs (B1, B2, G1, and G2) and residues in cereals using HPLC for low-ppb detection.…”

Section: Discussionmentioning

confidence: 99%

“…RAPD markers have detected genetic variability between the isolates of A. flavus and related species (Batista et al, 2008;Reddy et al, 2009;Irshad and Nawab, 2012). RAPD markers have been used to discriminate between aflatoxigenic from non-aflatoxigenic isolates of A. flavus (Lourenço et al, 2007;Gashgari et al, 2010). The inter-simple sequence repeat (ISSR) markers are highly polymorphic and are useful in studies on genetic diversity, phylogeny, gene tagging, genome mapping, and evolutionary biology (Reddy et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of Aspergillus flavus and aflatoxin contamination of wheat grains from Saudi Arabia

Alwadai

Al‐Othman

Mahmoud³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Twelve species belonging to six fungal genera were found to be associated with wheat (Triticum aestivum L.) grain samples collected from three main regions in Saudi Arabia. The most common genera (average frequency) were Aspergillus (14.3%), Fusarium (29.1%), Penicillium (9.3%), and Alternaria (8.2%). Nineteen isolates of Aspergillus flavus were screened for their ability to produce aflatoxins using HPLC. Thirteen isolates produced aflatoxins ranging from 0.5 to 2.6 µg/kg. Inter-simple sequence repeats (ISSR), and random amplified polymorphic DNA (RAPD) molecular markers were used, with the aim of genetically characterizing strains of A. flavus to discriminate between aflatoxigenic and non-aflatoxigenic isolates. RAPD and ISSR analysis revealed a high level of genetic diversity in the A. flavus population, useful for genetic characterization. Clustering based on RAPD and ISSR dendograms was unrelated to geographic origin. RAPD and ISSR markers were not suitable to discriminate aflatoxigenic and nonaflatoxigenic isolates, but ISSR primers were better compared to RAPD.

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Molecular Characterization of Mycobiota and Aflatoxin Contamination of Retail Wheat Flours from Jeddah Markets

Cited by 26 publications

References 71 publications

Molecular characterization of aflatoxigenic and non-aflatoxigenic Aspergillus flavus isolates collected from corn grains

Molecular characterization of aflatoxigenic and non-aflatoxigenic Aspergillus flavus isolates collected from corn grains

Genetic Variability of Citrinin-Producing Penicillium Citrinum Strains as Occupational Health Hazards in Northern Iran / Genska Varijabilnost Citrinin-Toksinogenih Sojeva Penicillium Citrinum Kao Izvor Opasnosti Za Zdravlje Profesionalno Izloženih Populacija U Sjevernom Iranu

Molecular characterization of Aspergillus flavus and aflatoxin contamination of wheat grains from Saudi Arabia

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