Molecular characterization of Newcastle disease virus (genotype VII) from broiler chickens in Egypt

Ewies, Samar S.; Ali, Ahmed; Tamam, S. M.; Madbouly, H. M.

doi:10.1016/j.bjbas.2017.04.004

Cited by 32 publications

(39 citation statements)

References 28 publications

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“…Sometimes edematous proventricular glands, hemorrhagic ulcers in the wall of intestine were common with virulent viscerotropic NDV. These findings confirmed the suspicious of NDV infection as previously mentioned by many authors (Alexander, 2009;Susta et al, 2010;Radwan et al, 2013;Ewies et al, 2017). Virus isolation was attempted on SPF ECE which represent an extremely sensitive method for the isolation of NDV (Alexander, 1989;OIE, 2008).…”

Section: Discussionsupporting

confidence: 81%

Isolation and Identification Of New Sub-Genotypes Of Virulent ND Virus In Broiler Chickens In Qalybia Governorate.

Desouky¹,

Saad

El-Shorbagy

et al. 2020

Benha Veterinary Medical Journal

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Newcastle disease (ND) is a highly contagious disease causing huge economic losses to the poultry industry due to high morbidities and mortalities. This study was aimed to isolate and characterize the new sub-genotype NDV strains in Qalybia Governorate during 2017 and 2019. Sixty-eight samples were collected from Sixty-eight broiler chicken flocks suffering from high mortality reach to 80% with distinctive clinical signs and post-mortem lesions of NDV. NDV strains isolated through inoculation in specific pathogen free (SPF) eggs and were identified by hemagglutination (HA) and hemagglutination inhibition (HI) techniques. There were 16/20 (about 80%) of selected suspected farm were positive for NDV With HI test. These hemagglutinating agents were confirmed as NDV strains through molecular characterization using one step RT-PCR targeting the partial F-gene of NDV. Sequencing and phylogenetic analysis were successfully performed with eight NDV isolates which possessed the motif 112 R/K-R-Q-K/RR116 at the C-terminus of the F2 protein and F (phenylalanine) at residue 117, indicating that they are velogenic genotype. The present study revealed the circulation of NDV class II genotype VII in Qalybia chicken flocks F protein Cleavage site

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Section: Discussionsupporting

confidence: 81%

Isolation and Identification Of New Sub-Genotypes Of Virulent ND Virus In Broiler Chickens In Qalybia Governorate.

Desouky¹,

Saad

El-Shorbagy

et al. 2020

Benha Veterinary Medical Journal

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“…Hence, there are some vaccine breaks from genotypes I and II [ 26 ]. In Egypt, NDV outbreaks occurred in several vaccinated and non-vaccinated poultry farms in different governorates since 2011 causing severe respiratory and nervous signs and high mortalities that resulted in severe economic losses in 3-4-week-old broiler chickens [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of full fusion protein (F) of Newcastle disease virus genotype VIId isolated from Egypt during 2012-2016

Selim¹,

Selim²,

Arafa³

et al. 2018

Vet World

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Aim:The aim of this work was to study the full F gene sequence of Newcastle disease virus (NDV) in regard to pathotyping and genotyping and to study the evolution of this NDV in Egypt.Materials and Methods:The present study was conducted using samples from seven suspected NDV flocks of vaccinated chickens during 2012-2016 from six governorates in Egypt. The NDV was successfully isolated from pathological specimens through inoculation in specific pathogen-free embryonated chicken eggs.Results:Pathogenicity of the NDV isolates has been estimated through intracerebral pathogenicity index and ranged from 1.66 to 1.73 which indicates the velogenic type of NDV isolates. Pathotyping and genotyping of these isolates were done through sequencing of full-length F gene. Results indicated that the seven NDV isolates showed characteristic cleavage site motif (112RRQKRF117) for the velogenic strains of NDV. Phylogenetic analysis of the F gene clustered these isolates within Group I of genotype VIId within Israeli strains NDV/IS/2015, NDV-Ch/SD883, and most of the Middle East strains. Six of seven sequenced isolates have six potential N-linked glycosylation sites. The neutralization epitope on the five antigenic sites of fusion is conserved in all Egyptian strains of this study except NDV-KFR-B7-2012 which has a substitution at D 170 N in epitope A4. In all our strains, 10 cysteine residues are recorded, except one loss of cysteine at residue 370 in both NDV-EG-35-2014 and NDV-GHB-328F-2016.Conclusion:All viruses in this study have 52 amino acid substitutions within fusion gene in compared with Lasota strain that reveals importance for its antigenic and structural function. The present work highlights the important need to sequence F gene of NDV genotype VIId to investigate the evolution of this NDV in Egypt.

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“…In Egypt, NDV was identified for the first time in 1948 (Daubney & Mansy, 1948). In the last decade, despite the intensive application of the NDV vaccination regiment, many velogenic NDV outbreaks have been recorded in different avian species such as chickens, ducks and pigeons (Ewies, Ali, Tamam, & Madbouly, 2017;Hussein et al, 2005;Mohamed, Kumar, Paldurai, & Samal, 2011;Radwan, Darwish, El-Sabagh, El-Sanousi, & Shalaby, 2013). Moreover, a recent study showed that velogenic NDV circulates in small-scale poultry holdings with the ability to transmit over long distances between farms (Moharam et al, 2019), which indicating a complex endemic situation for NDV in Egypt.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Genetic Analysis of Recent and Emergent Virulent Newcastle Disease Viruses in Egypt

Nagy¹,

Ali

El‐Abideen

et al. 2020

Transbound Emerg Dis

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Extensive vaccination against Newcastle disease virus (NDV) induced more selective immune pressure from hosts that enhanced the evolutionary process of NDV. Herein, we characterized 13 recently isolated NDV isolates from vaccinated chicken flocks during 2014–2017. Sequence analysis of F gene showed the presence of 112RRQKRF117 velogenic cleavage motif in 11 isolates, whereas the other two isolates (Ck/ME3/Eg/16 and Ck/ME5/Eg/16) showed the monobasic motif 112GGRQGRL117. Interestingly, isolate Ck/ME5/Eg/16 showed 100% and 99.82% nucleotide identity with the LaSota F gene hypervariable region and full‐length gene, respectively. On the other hand, isolate Ck/ME3/Eg/16 revealed natural recombination with strains NDV/Ck/Egypt/3/2006 and NDV/Teal/VRLCU/Egypt/2015 that indicates re‐emergence of that old strain. Interestingly, all 13 isolates showed high intracerebral pathogenicity (ICPI) and mean death time (MDT) despite the presence of lentogenic motif in both Ck/ME5/Eg/16 and Ck/ME3/Eg/16 isolates. Comparative analysis of F antigenic epitopes in our isolates with other published sequences from Egypt revealed high sequence conservation; recent isolates had one fixed amino acid substitution (K78R) and a novel V168I substitution, whereas a D170N substitution was detected in older strains (NDV‐EG‐35‐2014 and NDV‐KFR‐B7‐2012). Taken together, our results support the first isolation of virulent NDV isolates with a lentogenic motif; isolate Ck/ME5/Eg/16 might be generated in nature from LaSota live vaccine, whereas isolate Ck/ME3/Eg/16 is emergent from NDV/Ck/Egypt/3/2006. We conclude that the current diagnostic evaluation of the virulence of NDV isolates by characteristic amino acid residues at the F protein cleavage site is insufficient. There is a need to link virologic and epidemiologic data together, and routine and emergency LaSota vaccination protocols should be carefully and optimally applied, with regards to the timing and presence of co‐infecting agents in the field.

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Molecular characterization of Newcastle disease virus (genotype VII) from broiler chickens in Egypt

Cited by 32 publications

References 28 publications

Isolation and Identification Of New Sub-Genotypes Of Virulent ND Virus In Broiler Chickens In Qalybia Governorate.

Isolation and Identification Of New Sub-Genotypes Of Virulent ND Virus In Broiler Chickens In Qalybia Governorate.

Molecular characterization of full fusion protein (F) of Newcastle disease virus genotype VIId isolated from Egypt during 2012-2016

Characterization and Genetic Analysis of Recent and Emergent Virulent Newcastle Disease Viruses in Egypt

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