Molecular characterization of ochratoxigenic fungi associated with poultry feedstuffs in Saudi Arabia

Gherbawy, Youssuf A.; Elhariry, Hesham; Alamri, Saad; El‐Dawy, Eman G. A.

doi:10.1002/fsn3.1827

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…Aspergillus, Penicillium, and Fusarium are among the fungal genera that frequently contaminate dairy feeds and feed ingredients globally (Adelusi et al, 2022). A. flavus, A. parasiticus, and A. niger are among the genera of Aspergillus species that are predominantly contaminate feedstuffs destined to dairy cattle (Fusseini et al, 2016;Gherbawy et al, 2020;Nleya et al, 2021). In particular, A. flavus and A. parasiticus are a primary producer of aflatoxins, such as AFB 1 , AFB 2 , AFG 1 , and AFG 2 (Variane et al, 2018;Bouti et al, 2020), while certain strains of A. niger produces Ochratoxin A (El-Hamaky et al, 2016).…”

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confidence: 99%

Aspergillus species contamination in concentrate feeds collected from specialized dairy farms and local markets in selected urban centers of eastern Ethiopia

Tesfaye,

Mohammed,

Yusuf

et al. 2024

Front. Sustain. Food Syst.

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This study aimed to identify and examine the prevalence of Aspergillus species in three types of feed collected from specialized dairy farms and local markets in Chiro town, Dire Dawa, and Harar cities in eastern Ethiopia. A total of 180 dairy feed samples were collected and sown, initially on YES agar and then sub-cultured to AFPA to identify Aspergillus species based on colony color, conidia, vesicle, and phialide features. Additionally, the aflatoxigenic potential of the colonies was tested using CAM-based UV fluorescence. The study revealed that the prevalence of Aspergillus species was 80.6% in dairy feeds with a mean count of 3.04 log10cfu/g. Among the identified species, A. flavus was found to be highly prevalent (80%) in the feed with a mean of 2.73 log10cfu/g (7.45 × 103 cfu/g). Meanwhile, A. parasiticus and A. niger were observed in 73.3% (mean 2.43 log10cfu/g) and 58.3% (mean 1.68 log10cfu/g) of feeds, respectively. Furthermore, the prevalence rates of all Aspergillus species in feeds were not significant (p > 0.05) among the study sites and feed sources. However, the mean count of total Aspergillus (3.47 ± 1.34 log10cfu/g), A. flavus (3.20 ± 1.27 log10cfu/g), and A. parasiticus (2.82 ± 1.41 log10cfu/g) was significantly higher in feeds from Dire Dawa city. Additionally, both the prevalence rates and mean counts of total Aspergillus (37.9% and 3.65 ± 1.16 log10cfu/g), A. flavus (38.2% and 3.26 ± 1.12 log10cfu/g), A. parasiticus (38.6% and 2.98 ± 1.34 log10cfu/g), and A. niger (37.1% and 2.11 ± 1.57 log10cfu/g) in total mixed ration were significantly higher (p < 0.05) than in other feed types. Out of the screened Aspergillus colonies, 81.42% were found to be aflatoxigenic, with 58.32% belonging to A. flavus and 41.68% to A. parasiticus. Therefore, widespread contamination of Aspergillus species in dairy feeds across the study sites raises food safety and public health concerns, which highlights the urgent need for stringent measures in feed quality control to curb its prevalence and the risk of aflatoxin exposure.

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Section: Introductionmentioning

confidence: 99%

Aspergillus species contamination in concentrate feeds collected from specialized dairy farms and local markets in selected urban centers of eastern Ethiopia

Tesfaye,

Mohammed,

Yusuf

et al. 2024

Front. Sustain. Food Syst.

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Occurrence of Fusarium Species and Determination of Their Toxins From Poultry Feeds During Storage

Gherbawy,

Altalhi,

Ioan

et al. 2024

International Journal of Microbiology

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At a global scale, grains and poultry feeds are the primary sources of feed. Due to their considerable significance, any fungi capable of infecting these feedstuffs can pose a threat to both food safety and security. Fusarium spp. are a highly significant group of organisms. Fumonisins (FBs), deoxynivalenol (DON), trichothecene (T‐2), and zearalenone (ZEN) are classifications of mycotoxins that are synthesized by Fusarium species. Their presence is associated with a range of factors that occur during growth, processing, and storage. We have recorded the high occurrence of Fusarium spp. in grains and poultry feeds in all tested samples. Fusarium (F) oxysporum was the most common species that appeared in all tested two hundred samples. FB1 was the predominant toxin that appeared with the highest concentration in 56 pellet samples with the range of 10.34–1043 μg/kg. Also, it occurred with levels of 4.67–956 μg/kg in the tested ingredients samples. Fusarium verticillioides isolates were the highest producers of FB1. Fusarium spp. isolates showed positive FB1 production with 84.6%, 82.5%, 82.2%, and 78.1%, isolated from pellet feed samples that were collected from Alhassa, Jeddah, Qassim, and Riyadh, respectively. 31.6%, 76.9%, 23.1%, 83.3%, and 88% of tested Fusarium spp. strains exhibited FB1 production in samples of barley, corn, sorghum, soybean, and wheat bran, respectively, with the range of 18–655 μg/kg. Genes responsible for FB1, DON, T‐2, and ZEN production were detected in the Fusarium spp. isolates.

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Molecular characterization of ochratoxigenic fungi associated with poultry feedstuffs in Saudi Arabia

Abstract: Agricultural products including oilseed meals and cereals constitute a major component of poultry feed ingredients. Fungal contamination is widely distributed in tropical countries, where poultry production and processing are extending quickly (Okoli, Nweke,

Cited by 2 publications

References 40 publications

Aspergillus species contamination in concentrate feeds collected from specialized dairy farms and local markets in selected urban centers of eastern Ethiopia

Aspergillus species contamination in concentrate feeds collected from specialized dairy farms and local markets in selected urban centers of eastern Ethiopia

Occurrence of Fusarium Species and Determination of Their Toxins From Poultry Feeds During Storage

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