Molecular Characterization of Organic Nitrogen in Cattle Manure Compost by Size-Exclusion HPLC with Chemiluminescent Nitrogen Detection

Matsunaga, Toshiro; Moriizumi, Mihoko; Kameda, Tsunenori

doi:10.2116/analsci.29.923

Cited by 3 publications

(1 citation statement)

References 20 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Judging the disease suppression capability of compost based on presence of these biochemical compounds is a costly affair, as it involves highly sophisticated instruments like HPLC, GC-MS, etc. (Matsunaga et al 2013;Fels et al 2014). Microbe secretes majority of these useful biomolecules during the composting, so if we can detect presence of the beneficial microbes in the compost, we can correlate it to disease suppression.…”

Section: Introductionmentioning

confidence: 99%

Development of PCR-based molecular marker for screening of disease-suppressive composts against Fusarium wilt of tomato (Solanum lycopersicum L.)

et al. 2018

View full text Add to dashboard Cite

The present study was carried out to develop a PCR-based molecular marker suitable for screening of disease-suppressive composts against Fusarium wilt of tomato. An effective uncultured bacterial community was screened from our previous study on investigation of microbial communities in composts for their potential for biocontrol of Fusarium wilt. Based on available sequence information (Accession no. HQ388491) of selective community, PCR-based molecular markers were designed and tested for their specificity in different compost sample. To confirm specificity of designed marker, real-time reverse transcription-PCR (qRT-PCR) analysis was performed. Selective marker efficacy was further tested for different set of composts and results were cross-verified by conducting bioassay of same composts against Fusarium wilt in tomato crop. Results showed that out of two designed set of primers (i.e., PAC1F/PAC1R and PAC4F/PAC4R), primer set PAC4F/PAC4R resulted in successful amplification of 199 bp in highly disease-suppressive compost (i.e., CPP); however, no/below detection level amplification was observed in non-suppressive compost (JC). qRT-PCR analysis confirmed the specificity of selective marker by representing single peak in melting curve. A clear difference was observed in relative population of selective community in different set of composts. It was observed maximum in the most effective compost, i.e., CPP followed by other disease-suppressive composts. Cross-examination of results with bioassay confirmed that composts with presence of selective bacterial community having no/very less disease incidence of Fusarium. It is clearly evident from the study that such kind of molecular markers can be developed and used in future research focusing on compost-based disease suppression.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of PCR-based molecular marker for screening of disease-suppressive composts against Fusarium wilt of tomato (Solanum lycopersicum L.)

et al. 2018

View full text Add to dashboard Cite

show abstract

Protein hydrolysate from organic fraction of municipal solid waste compost as nitrogen source to produce lactic acid by Lactobacillus fermentum ATCC 9338 and Lactobacillus plantarum NCIMB 8826

Izaguirre

Dietrich²,

Castañón

2020

Process Biochemistry

View full text Add to dashboard Cite

Soil fertility and plant nutrition in an organic olive orchard after 5 years of amendment with compost, biochar or their blend

Fornes,

Lidón,

Belda

et al. 2024

Sci Rep

View full text Add to dashboard Cite

The agronomic use of compost and biochar as soil amendments may exhibit contrasting results in terms of soil fertility and plant nutrition. The effects of the biennial application of biochar, compost and a blend of compost:biochar (90:10; % dw:dw) on the agronomical performance of an organically managed and well established 25-year-old olive orchard was assessed 5 years after the initial application. The agronomical evaluation was based on the assessment of the soil physical, chemical, and biological characteristics, and the assessment of the soil fertility by both crop production and nutritional status of the orchard, and the bioassay with olive plantlets. Biochar mainly benefited the physical properties (bulk density, total porosity, aeration, water retention capacity) of soil, especially in the top 0–5 cm. Compost and its blend with biochar improved microbial activity, soil nutritional status (increasing the content of soluble organic C, N, and P) and favoured the formation of aggregates in soil. The bioassay conducted with young plantlets confirmed the enhanced soil fertility status in the three amended treatments, particularly in the case of biochar and its blend with compost. However, this effect was not significantly observed in the adult plants after 5 years of application, reflecting the slow response of adult olive trees to changes in fertilization. Based on these results, alongside the desirable long-residence time of biochar in soil and the ready availability of compost, the blend of biochar with compost assayed in this study is defined as a valid strategy for preparing high quality soil organic amendments.

show abstract

Molecular Characterization of Organic Nitrogen in Cattle Manure Compost by Size-Exclusion HPLC with Chemiluminescent Nitrogen Detection

Cited by 3 publications

References 20 publications

Development of PCR-based molecular marker for screening of disease-suppressive composts against Fusarium wilt of tomato (Solanum lycopersicum L.)

Development of PCR-based molecular marker for screening of disease-suppressive composts against Fusarium wilt of tomato (Solanum lycopersicum L.)

Protein hydrolysate from organic fraction of municipal solid waste compost as nitrogen source to produce lactic acid by Lactobacillus fermentum ATCC 9338 and Lactobacillus plantarum NCIMB 8826

Soil fertility and plant nutrition in an organic olive orchard after 5 years of amendment with compost, biochar or their blend

Contact Info

Product

Resources

About