Molecular characterization of ovary-specific gene Mrfem-1 and siRNA-mediated regulation on targeting Mrfem-1 in the giant freshwater prawn, Macrobrachium rosenbergii

Zhou, Lingxia; Xue, Lixiang; Ye, Baoqing; Liu, Yun; Tan, Shuang-Pei; Knepper, M. A.; Qiu, Gao‐Feng

doi:10.1016/j.gene.2020.144891

Cited by 12 publications

(3 citation statements)

References 30 publications

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“…Fem-1 is characterized by one of the most common protein–protein interaction motifs and ankyrin repeat motifs and known as a regulatory factor of signal transduction in the sex determination signaling pathway ( 21 , 37 , 38 , 49 ). In M. rosenbergii , Mrfem-1 was exclusively expressed in the ovary, suggesting that Mrfem-1 might be associated with ovarian maturation in prawn ( 50 ). An ovary-specific gene Fem-1 homolog, Mnfem-1 , has been identified in the oriental river prawn, M. nipponense ( 51 ).…”

Section: Discussionmentioning

confidence: 99%

Full Functional Sex Reversal Achieved Through Silencing of MroDmrt11E Gene in Macrobrachium rosenbergii: Production of All-Male Monosex Freshwater Prawn

Chen

Wang

et al. 2022

Front. Endocrinol.

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The freshwater prawn Macrobrachium rosenbergii is one kind of important economic aquaculture species and displays remarkable sexual dimorphism. The molecular mechanism of sexual differentiation in M. rosenbergii has been primarily unraveled through the research efforts of the androgenic gland and its related genes. However, the understanding of conserved genes involved in the molecular mechanism underpinning sex determination and sexual differentiation of M. rosenbergii is still fragmentary. MroDmrt11E is a member of the doublesex and mab-3-related transcription factor (Dmrt) gene family and is prominently expressed in the testis. In the present study, in vivo knockdown of MroDmrt11E at the postlarva stage in male prawn induced a complete and functional sex reversal and achieved the production of an all-male monosex population. Furthermore, a great deal of new information of upregulated and downregulated transcriptions involved in sexual differentiation of MroDmrt11E knockdown was enriched by comparative transcriptomic analysis. The effects of RNAi-mediated gene knockdown of MroDmrt11E on the differentially expressed and sex-related candidate genes, such as transformer, fruitless, feminization, insulin-like androgenic gland gene, Dmrt gene family, were primarily focused on, and their possible molecular regulatory relationships in sexual differentiation were analyzed. Meanwhile, the response of primary Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathways was investigated to expound the potential roles of MroDmrt11E in male sexual differentiation, which provided a deeper understanding of the molecular regulatory network underlying sexual differentiation of M. rosenbergii. The finding provided a novel sexual manipulation technique through silencing of Dmrt gene family for achieving a complete and functional sex reversal and offered a new insight regarding the mechanism of the Dmrt gene family in the sexual differentiation of crustaceans.

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Section: Discussionmentioning

confidence: 99%

Full Functional Sex Reversal Achieved Through Silencing of MroDmrt11E Gene in Macrobrachium rosenbergii: Production of All-Male Monosex Freshwater Prawn

Chen

Wang

et al. 2022

Front. Endocrinol.

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“…It was observed that the sexually dimorphic expression pattern is related to the development of sexual phenotype and the formation of germ cells [12]. Mrfem-1 and Mnfem-1 genes have specific strong positive signals in the ovary of Macrobrachium rosenbergii and Macrobrachium nipponense, and Mnfem-1 can interact with proteins containing ankyrin, cathepsin L, insulinase or ubiquitin, indicating that Mnfem-1 and Mrfem-1 could function in prawn sexual development and ovarian development [13,14]. The fem-1c gene has also been identified in Hyriopsis cumingii and E. sinensis differently regulating gonadal differentiation development.…”

Section: Introductionmentioning

confidence: 99%

Eriocheir sinensis feminization-1c (Fem-1c) and Its Predicted miRNAs Involved in Sexual Development and Regulation

Zhu

Feng

et al. 2023

Animals

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Feminization-1c (Fem-1c) is important for sex differentiation in the model organism Caenorhabditis elegans. In our previous study, the basic molecular characteristics of the Fem-1c gene (EsFem-1c) in Eriocheir sinensis (Henri Milne Edwards, 1854) were cloned to determine the relationship with sex differentiation. In this study, the genomic sequence of EsFem-1c contained five exons and four introns, with an exceptionally long 3′UTR sequence. The qRT-PCR results of EsFem-1c demonstrated lower tissue expression in the androgenic gland of the intersex crab than the normal male crab, implying that EsFem-1c plays a role in crab AG development. RNA interference experiments and morphological observations of juvenile and mature crabs indicated that EsFem-1c influences sexual development in E. sinensis. A dual-luciferase reporter assay disclosed that tcf-miR-315-5p effectively inhibits the translation of the EsFem-1c gene, influencing male development. An intriguing finding was that miRNA tcf-miR-307 could increase EsFem-1c expression by binding to the alternative splicing region with a length of 248 bp (ASR-248) in the 3′UTR sequence. The present research contributes to a better understanding of the molecular regulation mechanism of EsFem-1c and provides a resource for future studies of the miRNA-mediated regulation of sexual development and regulation in E. sinensis.

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“…IAG, Sox, Dmrt, Dsx, Vasa, and Slx exhibit male-biased expression patterns and play crucial roles in testis development and spermatogenesis. CFSH, Fem-1, Wnt4, FAMeT, Slo, Tra-2, BMPs, UCHLs, Erk2, Cdc2, EGFR, Vg, VgR, and VIH have higher expression levels in females and play important roles during ovarian development (Xu et al, 2018;Abayed et al, 2019;Jin et al, 2019;Shi L. et al, 2019;Zheng et al, 2019;Jiang et al, 2020a;Zhou et al, 2020;Farhadi et al, 2021b). Therefore, identification of sex-related genes and characterization of their functions are crucial steps for understanding the sex determination and sexual differentiation mechanism in crustaceans.…”

Section: Introductionmentioning

confidence: 99%

A Novel Imprinted Gene (Sp-Pol) With Sex-Specific SNP Locus and Sex-Biased Expression Pattern Provides Insights Into the Gonad Development of Mud Crab (Scylla paramamosain)

et al. 2021

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Identification and exploring the role of novel sex-related genes is a crucial step for understanding the regulation mechanism of sexual development. In the present study, we identified a novel sex-related gene (designated as Sp-Pol) upstream of a sex-specific single nucleotide polymorphism (SNP). SNP1888 together with Sp-Pol were mapped on LG32 (which is a sex-related linkage group) of a high-density genetic map. The full-length cDNA of Sp-Pol consists of 1703 bp with an open reading frame (ORF) encoding 204 amino acids, a 344 bp 5′-UTR and a 744 bp 3′-UTR. Phylogenetic analysis showed that Sp-Pol may need to be classified as a new gene family due to the very low sequence identity with other known genes (less than 25% identity). The sex-biased expression pattern of Sp-Pol starts from crablet stage V (C5) with approximately three- to four-fold higher in males than in females. Sp-Pol was expressed at a higher level in gonads compared to other tissues, with the highest expression level in the testis. In testis, a downward trend was observed in the expression level of Sp-Pol from the testis stage I (T1) to testis stage III (T3). After unilateral eyestalk ablation, the expression level of Sp-Pol significantly increased in testis and hepatopancreas in males, while it was downregulated in the hepatopancreas of females. Fluorescence in situ hybridization (FISH) assay revealed that Sp-Pol transcripts were strongly localized in the epithelia of seminiferous tubules of the testis, and in the ovary, it was detected in the oogonium cells. These findings showed that Sp-Pol may play crucial roles in the gonad development of S. paramamosain.

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Molecular characterization of ovary-specific gene Mrfem-1 and siRNA-mediated regulation on targeting Mrfem-1 in the giant freshwater prawn, Macrobrachium rosenbergii

Cited by 12 publications

References 30 publications

Full Functional Sex Reversal Achieved Through Silencing of MroDmrt11E Gene in Macrobrachium rosenbergii: Production of All-Male Monosex Freshwater Prawn

Full Functional Sex Reversal Achieved Through Silencing of MroDmrt11E Gene in Macrobrachium rosenbergii: Production of All-Male Monosex Freshwater Prawn

Eriocheir sinensis feminization-1c (Fem-1c) and Its Predicted miRNAs Involved in Sexual Development and Regulation

A Novel Imprinted Gene (Sp-Pol) With Sex-Specific SNP Locus and Sex-Biased Expression Pattern Provides Insights Into the Gonad Development of Mud Crab (Scylla paramamosain)

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