Molecular characterization of parental lines and validation of SNP markers for anthracnose and angular leaf spot in common bean

Gomes-Messias, Lucas Matias; Vianello, R. P.; Monteiro-Júnior, Joney Pereira; Rodrigues, Luiz; Mota, A. P. S.; Pereira, H. S.; Melo, L. C.; Raatz, Bodo; Souza, T. L. P. O. de

doi:10.1007/s10681-022-03002-2

Cited by 3 publications

(6 citation statements)

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“…The overall size of the map was 3069 cM (Table 3) (Figure S1), with a mean distance of ∼4.9 cM. Diverse mapping studies in common bean have generated genetic maps with mean sizes ranging from 754 to 4474 cM (Gomes‐Messias et al., 2022; Langat et al., 2019).…”

Section: Resultsmentioning

confidence: 99%

“…A further step toward MAS involves validating associated markers by utilizing them to select diverse genotypes (Gomes‐Messias et al., 2022). In the present study, the validation of FOP2.3 403H allele‐specific markers was conducted by evaluating a progeny derived from a cross in which the resistant parent (BRS FP403) used in QTL identification was crossed with another susceptible parent (BRS Esteio), aiming to verify the occurrence and efficiency of molecular markers co‐segregating with the resistance loci.…”

Section: Resultsmentioning

confidence: 99%

“…For the common bean breeding program, utilizing these tools will enable the execution of multiple selection cycles within a single year. This approach can be combined with MAS for other traits such as slow seed coat darkening (Alvares et al., 2019) and anthracnose resistance (Gomes‐Messias et al., 2022; Vieira et al., 2018), allowing for concurrent selection of multiple traits. This approach delivers substantial time savings, heightened efficiency, and cost reduction in new line development.…”

Section: Resultsmentioning

confidence: 99%

“…Souza et al (2018) and Silva et al (2022) developed the cultivars BRS FC401 RMD and BRS FC420 RMD, both of which are transgenic and possess multiple resistances against viral diseases (bean golden mosaic virus, bean common mosaic virus, and cowpea mild mottle virus). More recently, Gomes-Messias et al (2022) validated two markers linked to anthracnose resistance (Co-4) with a high selection efficiency (≥99%). Additionally, Alvares et al (2019) identified and validated a marker associated with the Sd (slow darkening) gene, responsible for seed coat darkening, which has also been applied in MAS.…”

Section: Core Ideasmentioning

confidence: 99%

“…More recently, Gomes‐Messias et al. (2022) validated two markers linked to anthracnose resistance ( Co‐4 ) with a high selection efficiency (≥99%). Additionally, Alvares et al.…”

Section: Introductionmentioning

confidence: 99%

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Identification of QTLs and validation of molecular markers associated with reaction to Fusarium wilt in the common bean cultivar BRS FP403

Torres,

Vianello,

de Souza

et al. 2024

Crop Science

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Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. phaseoli, is one of the most prevalent diseases in the common bean crop (Phaseolus vulgaris). However, there is little information regarding the genetics of reaction to this disease. Thus, the aims of this study were to estimate genetic parameters, identify quantitative trait loci (QTLs) associated with reaction to Fusarium wilt, and validate the markers identified. A total of 165 progenies of the population BRS FP403 × BRS Horizonte were evaluated. Evaluation was made in 2016 and 2017 in field trials in an area infested with the pathogen in Santo Antônio de Goiás, Brazil. Genotyping of single nucleotide polymorphism and SilicoDArT, where DArT is Diversity Arrays Technology, markers using the DArTseq technology was performed in the F2 generation. Analyses of the phenotypic data indicated the presence of variability among the progenies and the possibility of success from selection, with high estimates of heritability (90%) and gain from selection (37%). A genetic map was obtained with 702 markers and spanned 3069 cM. Five QTLs associated with reaction to Fusarium wilt were identified on five chromosomes. There was interaction between the QTLs and years. Through joint analysis, four QTLs were identified on chromosomes Pv01, Pv02, Pv03, and Pv04, explaining 5.8%–40.5% of the variation. The QTL FOP2.3403H explained 40.5% of phenotypic variation and was stable over the 2 years. From this QTL, two hydrolysis probes were developed for genotyping in a panel of lines for validation. Selection efficiency was over 92%, indicating considerable potential for use in marker‐assisted selection.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Core Ideasmentioning

confidence: 99%

“…More recently, Gomes‐Messias et al. (2022) validated two markers linked to anthracnose resistance ( Co‐4 ) with a high selection efficiency (≥99%). Additionally, Alvares et al.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification of QTLs and validation of molecular markers associated with reaction to Fusarium wilt in the common bean cultivar BRS FP403

Torres,

Vianello,

de Souza

et al. 2024

Crop Science

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Fine mapping of a new common bean anthracnose resistance gene (Co-18) to the proximal end of Pv10 in Indian landrace KRC-5

Lateef,

Katoch,

Katoch

et al. 2024

Theor Appl Genet

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Whole-genome resequencing of common bean elite breeding lines

Souza

Azevedo

Coelho

et al. 2023

Sci Rep

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The expansion of bean genome technologies has prompted new perspectives on generating resources and knowledge essential to research and implementing biotechnological tools for the practical operations of plant breeding programs. This study aimed to resequence the entire genome (whole genome sequencing—WGS) of 40 bean genotypes selected based on their significance in breeding programs worldwide, with the objective of generating an extensive database for the identification of single nucleotide polymorphisms (SNPs). Over 6 million SNPs were identified, distributed across the 11 bean chromosomes. After quality variant filtering, 420,509 high-quality SNPs were established, with an average of 38,228 SNPs per chromosome. These variants were categorized based on their predicted effects, revealing that the majority exerted a modifier impact on non-coding genome regions (94.68%). Notably, a significant proportion of SNPs occurred in intergenic regions (62.89%) and at least one SNP was identified in 58.63% of the genes annotated in the bean genome. Of particular interest, 7841 SNPs were identified in 85% of the putative plant disease defense-related genes, presenting a valuable resource for crop breeding efforts. These findings provide a foundation for the development of innovative and broadly applicable technologies for the routine selection of superior genotypes in global bean improvement and germplasm characterization programs.

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Molecular characterization of parental lines and validation of SNP markers for anthracnose and angular leaf spot in common bean

Cited by 3 publications

References 53 publications

Identification of QTLs and validation of molecular markers associated with reaction to Fusarium wilt in the common bean cultivar BRS FP403

Identification of QTLs and validation of molecular markers associated with reaction to Fusarium wilt in the common bean cultivar BRS FP403

Fine mapping of a new common bean anthracnose resistance gene (Co-18) to the proximal end of Pv10 in Indian landrace KRC-5

Whole-genome resequencing of common bean elite breeding lines

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