Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

Itagaki, Tadashi; Kikawa, Masayuki; Terasaki, Kunio; Shibahara, Toshiyuki; Fukuda, Koichi

doi:10.1292/jvms.67.1115

Cited by 95 publications

(80 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding was also shown in the research by Mohanta from Bangladesh (17). Studies showed that molecular phylogeny with mitochondrial DNA can be effectively used for appropriate differentiation of haplotypes (12,18,20).…”

Section: Discussionsupporting

confidence: 66%

“…using high pure PCR template preparation kit (Dynabio®, Takapouzist, Iran), according to the manufacturer's instructions, and stored at -20°C until use. The ITS1 region, as a nuclear marker, was amplified with primers named ITS1-F and ITS1-R and fragments of mitochondrial target (ND1) by polymerase chain reaction (PCR) (18,19). Total volume of the reaction was 40 µL containing 4 µL DNA template, 14 µL distilled water, 10 pmol of each primer, and 20 µL master mix (amplicon®).…”

Section: Dna Extraction and Amplificationmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Fasciola spp. in the East of Iran, Based on the Spermatogenesis and Nuclear Ribosomal DNA (ITS1) and Mitochondrial (ND1) Genes

Rouhani

Raeghi

Harandi

et al. 2017

Arch Clin Infect Dis

View full text Add to dashboard Cite

Background: Fascioliasis is a neglected zoonotic disease with worldwide distribution. Phylogenetic analysis of Fasciola species is critical because of their different epidemiology. Objectives: The current study aimed at identifying Fasciola spp. based on spermatogenesis and phylogenetic analysis of mitochondrial (ND1) gene. Methods: One hundred and fifty adults with fascioliasis were selected from the Eastern provinces of Iran, based on spermatogenetic ability, measurement criteria, and ITS (nuclear ribosomal DNA internal transcribed spacers) 1 gene restriction fragment length polymorphism (RFLP) pattern. Then, their genetic diversity indices and phylogenetic relationships were analyzed using mitochondrial DNA marker of Fasciola population spread in the East of Iran. Results: Aspermic F. gigantica was observed in the eastern part of Iran. Partial sequences of mtDNA showed new haplotypes in both species. Pairwise fixation index between different F. gigantica populations calculated from the nucleotide data set of ND1 gene were statistically significant and showed the genetic differences in pairwise population. Conclusions:The results of the study showed that F. hepatica, lives in this region of Iran, had different genetic structures compared with the other Fasciola populations in the world. Fasciola gigantica present in the East of Iran had different genetic structures, compared with the other Fasciola population, based on genetic index.

show abstract

Section: Discussionsupporting

confidence: 66%

Section: Dna Extraction and Amplificationmentioning

confidence: 99%

Identification of Fasciola spp. in the East of Iran, Based on the Spermatogenesis and Nuclear Ribosomal DNA (ITS1) and Mitochondrial (ND1) Genes

Rouhani

Raeghi

Harandi

et al. 2017

Arch Clin Infect Dis

View full text Add to dashboard Cite

show abstract

“…Aspermic Fasciola species are distributed throughout Asian countries, including Japan [7,12], Korea [10], China [15], Vietnam [11], and Myanmar [8]. Particularly in the former 2 countries, all of the Fasciola flukes analyzed were aspermic [7,10,12,18].…”

mentioning

confidence: 99%

Molecular Analysis of Aspermic <i>Fasciola</i> Flukes from Korea on the Basis of the Nuclear ITS1 Region and Mitochondrial DNA Markers and Comparison with Japanese Aspermic <i>Fasciola</i> Flukes

Ichikawa

Itagaki

2012

J. Vet. Med. Sci.

Self Cite

View full text Add to dashboard Cite

ABSTRACT. It has been speculated that populations of aspermic Fasciola flukes in Korea and Japan have a close phylogenetic relationship. To evaluate this, we analyzed 33 Korean aspermic Fasciola flukes on the basis of nuclear ribosomal internal transcribed spacer 1 (ITS1) and mitochondrial NADH dehydrogenase subunit 1 (nad1) and cytochrome c oxidase 1 (cox1) sequences. Fh, Fg, and Fh/Fg types were detected in the ITS1 region and displayed the fragment patterns of F. hepatica, F. gigantica, and both species, respectively by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Additionally, three concatenated haplotypes of nad1 and cox1(nad1/cox1) were detected, and 2 of these, Kor1/Kor1 (Fsp1/Fsp1) haplotype and Kor2a/Kor2 (Fsp2/Fsp2) haplotype, were shared by Korean and Japanese aspermic flukes. The Fst value (0.019), calculated using the concatenated sequences, indicated that Korean and Japanese aspermic Fasciola populations were genetically undifferentiated. Interestingly, a combination of the Fh/Fg type and Kor1/Kor1 haplotype was found at the highest frequency in Korean aspermic flukes, whereas the Fg type and Fsp2/ Fsp2 haplotype combination was found at a conspicuously high frequency in Japanese aspermic flukes. This indicates that a founder effect caused by the introduction of infected hosts may have played a key role in the introduction of aspermic Fasciola flukes from Korea into Japan. KEY WORDS: aspermic Fasciola fluke, cox1, ITS1, nad1.

show abstract

“…Although Fasciola specimens in Japan have been considered to be of the same origin as that of aspermic Fasciola specimens in Korea, China and Vietnam [7][8][9]16], the frequency of DNA type combinations in Japanese specimens apparently differs from that in those three countries (e.g., the combination of Fsp1/2 in ITS1 and Fsp2 in ND1 is conspic- Table 1. ), M: 100 bp DNA ladder.…”

mentioning

confidence: 99%

DNA Types of Aspermic Fasciola Species in Japan

Ichikawa

Iwata

Itagaki

2010

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

ABSTRACT. In order to reveal DNA types of aspermic Fasciola forms in Japan, Fasciola specimens obtained from eight prefectures that had not been previously reported were analyzed for DNA of ribosomal internal transcribed spacer 1 (ITS1) and mitochondrial NADH dehydrogenase 1 (ND1) gene. Five combinations in DNA types of both ITS1 and ND1 were revealed from the results of this study and previous studies. The DNA type Fsp2, which is identical to that of F. gigantica in both ITS1 and ND1, was the most predominant in Japan, followed by Fsp1, which is the same DNA type as that of F. hepatica. Fasciola forms with Fsp1 mainly occurred in the northern region of Japan and those with Fsp2 were mainly in the western region. The founder effect related to migration of definitive host and susceptibility of intermediate host snail might play an important role in both geographical distribution and frequency of DNA types in Japanese Fasciola specimens.

show abstract

Molecular Characterization of Parthenogenic Fasciola sp. in Korea on the Basis of DNA Sequences of Ribosomal ITS1 and Mitochondrial NDI Gene

Cited by 95 publications

References 14 publications

Identification of Fasciola spp. in the East of Iran, Based on the Spermatogenesis and Nuclear Ribosomal DNA (ITS1) and Mitochondrial (ND1) Genes

Identification of Fasciola spp. in the East of Iran, Based on the Spermatogenesis and Nuclear Ribosomal DNA (ITS1) and Mitochondrial (ND1) Genes

Molecular Analysis of Aspermic <i>Fasciola</i> Flukes from Korea on the Basis of the Nuclear ITS1 Region and Mitochondrial DNA Markers and Comparison with Japanese Aspermic <i>Fasciola</i> Flukes

DNA Types of Aspermic Fasciola Species in Japan

Contact Info

Product

Resources

About