Molecular characterization of Platonia insignis Mart. (“Bacurizeiro”) using inter simple sequence repeat (ISSR) markers

Souza, Isis Gomes de Brito; Souza, Valeria; Lima, P. S. da C.

doi:10.1007/s11033-012-2462-6

Cited by 24 publications

(26 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The genetic diversity parameters of the nine studied H. hainanensis populations are shown in Table 3 The level of genetic diversity of a species reflects its ability to adapt to the environment, so the higher the genetic diversity, the stronger the species' adaptability to the environment [10,11]. It is believed that the genetic diversity of endangered species, endemic species and narrow-field species is low [12,13].…”

Section: Genetic Diversitymentioning

confidence: 99%

“…Species conservation is essential to protect the genetic diversity and evolutionary potential. The higher the genetic diversity, the stronger the species' adaptability to environments and the greater the evolutionary potential [11,31,32]. Because of the high degree of genetic diversity in H. hainanensis at the species level, especially within populations, we believe that there is still high adaptability and evolutionary potential in the natural H. hainanensis population, and thus, in situ conservation should be the primary measure.…”

Section: The Endangering Mechanism For H Hainanensis and Protection mentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity and structure analysis of the endangered plant speciesHorsfieldia hainanensisMerr. in China

Jiang

Liu²,

Xiang

et al. 2017

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

The genetic diversity and structure of nine natural populations of Horsfieldia hainanensis Merr., an endangered plant endemic to China, were studied using inter-simple sequence repeat markers. Nine primers were selected from 100 primers to evaluate 126 individual plants, from which a total of 136 bands were amplified and 108 bands were polymorphic. Our results demonstrated that the genetic diversity level of H. hainanensis was high with a percentage of polymorphic bands, Shannon's diversity index and Nei's genetic diversity index at the species level of 79.4%, 0.4787 and 0.3314, respectively, and correspondingly, averages of 40.4%, 0.2615 and 0.1843 at the population level. Significant genetic differentiation was observed among populations, showing that the coefficient of genetic differentiation among populations calculated using Nei's genetic diversity was 0.4509. The ranges of Nei's genetic identity and genetic distance among populations were 0.7387-0.8637 and 0.1466-0.3029, respectively. The unweighted pair group method with arithmetic mean clustering based on Nei's genetic distance indicated that nine natural H. hainanensis populations could be classified into two lineages. Collectively, we speculated that habitat fragmentation and disturbance from human activities could be considered the main reasons for the endangerment of H. hainanensis, and we propose in situ conservation for the existing natural H. hainanensis populations, especially at Mengtun, Niandou and Tongbiguan, where the genetic diversity is relatively high.

show abstract

Section: Genetic Diversitymentioning

confidence: 99%

Section: The Endangering Mechanism For H Hainanensis and Protection mentioning

confidence: 99%

Genetic diversity and structure analysis of the endangered plant speciesHorsfieldia hainanensisMerr. in China

Jiang

Liu²,

Xiang

et al. 2017

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

show abstract

“…Advancing the genetic breeding programs of the species may be achieved using molecular markers. Our evaluation of nanche accessions of the Embrapa Eastern Amazon BAG using ISSR markers revealed the need to identify specific temperatures for each of the primers used, as has previously been observed in other species (Pessoni, 2007;Souza et al, 2013;Pepineli et al, 2014). In a similar study conducted for Platonia insignis, 72 genotypes from the Embrapa Mid-North germplasm collection were evaluated using 18 primers.…”

Section: Discussionmentioning

confidence: 84%

“…In a similar study conducted for Platonia insignis, 72 genotypes from the Embrapa Mid-North germplasm collection were evaluated using 18 primers. An average of 13 loci per primer was identified (Souza et al, 2013). Among the primers used, seven primers (UBC 811, UBC 825, UBC 826, UBC 855, UBC 856, UBC 888, and UBC 890) were also used in the current study.…”

Section: Discussionmentioning

confidence: 99%

Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers

Rodrigues¹,

Moura²,

Ramos³

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Native of the Amazon, the nanche (Byrsonima crassifolia) is a fruit cultivated by family farmers and used in cooking; as such, it represents an opportunity for regional agribusiness. The Embrapa Eastern Amazon set up an active germplasm bank (BAG) consisting of 22 accessions sampled in 11 municipalities of Pará State. Due to its economic potential, there is an interest to advance the genetic breeding program of this species. The aim of this study was to characterize the BAG nanche collection using inter-simple sequence repeat (ISSR) markers. Accessions were genotyped using 23 preselected ISSR primers resulting in 109 amplified polymorphic and 51 monomorphic bands. With eight polymorphic bands each, the most polymorphic primers were UBC 809 and UBC 848. An unweighted pair-group method with arithmetic average cluster analysis based on Jaccard's coefficient indicated that the individuals clustered into two distinct groups. Accessions Igarapé Açu-2 and Augusto Corrêa-Pl 1 were most similar. The genetic dissimilarity values ranged from 0.10 to 0.59. We conclude that the ISSR markers were efficient in detecting polymorphisms in the nanche accessions, and that it is possible to infer the genetic variability among accessions of the collection. This demonstrate the importance of using molecular markers in poorly studied species and the advantages that this information can bring to the genetic improvement of such species.

show abstract

“…These markers have the potential to be integrated in breeding programs. They have been used in the characterization and maintenance of germplasm to estimate the extension of genetic diversity in several species, to resolve problems related to phylogeny, for genomic mapping, and in studies of evolutionary biology (Vijayan, 2005;Souza et al, 2012;Yu et al, 2014). However, ISSR markers have not been used in genetic studies of L. pisonis.…”

Section: Introductionmentioning

confidence: 99%

Investigating genetic diversity in sapucaia using inter simple sequence repeat markers

Borges¹,

Santos²,

Maia³

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Sapucaia is a tree species originating from the Brazilian Amazon and is widely distributed in Brazil, especially in the mid-north region (Piauí and Maranhão states). Its seeds are rich in calories and proteins, and possess great potential for commercialization. Little is known about the genetic variability in the germplasm of most Lecythis species. Here, 11 inter-simple sequence repeat primers were used to estimate the genetic variability among 17 accessions, and to determine the levels of genetic variation and the standards of population structure in sapucaia. The accessions were obtained from the active germplasm bank (AGB) of Embrapa Meio-Norte, Teresina, PI, Brazil, and corresponded to four occurrence areas. Ninety-six loci were analyzed 2 R.C. Borges et al. Genetics and Molecular Research 15 (3): gmr.15038565 among the studied individuals. High variation was found at the species level, where the percentage of polymorphic bands was 94.79%, Nei's genetic diversity (h) was 0.3110, and Shannon's index (I) was 0.4732. In the analyzed populations, the percentage polymorphism ranged from 20.83 to 94.79%, Nei's genetic diversity ranged from 0.0863 to 0.2969, and Shannon's index ranged from 0.1260 to 0.4457. Significant genetic differentiation was detected among the populations (Φ ST = 10.66%); however, the greatest genetic differentiation was found within the populations (89.34%), between which there was an intermediate level of gene flow (N m = 1.10). Accessions BGS 2 and BGS 4 were the most divergent, whereas accessions BGS 14 and BGS 15 were the most similar. Therefore, sapucaia analyzed from the AGB present an elevated level of genetic diversity and may have potential use in genetic breeding programs.

show abstract

Molecular characterization of Platonia insignis Mart. (“Bacurizeiro”) using inter simple sequence repeat (ISSR) markers

Cited by 24 publications

References 32 publications

Genetic diversity and structure analysis of the endangered plant speciesHorsfieldia hainanensisMerr. in China

Genetic diversity and structure analysis of the endangered plant speciesHorsfieldia hainanensisMerr. in China

Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers

Investigating genetic diversity in sapucaia using inter simple sequence repeat markers

Contact Info

Product

Resources

About