Molecular Characterization of Plum Pox Virus Isolated From Apricots, Plums and Peaches in Chile

Rosales, Merlín; Hinrichsen, Patricio; Herrera, G.

doi:10.17660/actahortic.1998.472.47

Cited by 24 publications

(10 citation statements)

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“…In almost every country where PPV has been found, over 40 countries in total (García & Cambra, 2007;CABI, 2013), and where strain typing has been conducted, isolates of strain D often comprise part of the virus population. Strain D isolates have been detected globally, including in North America (Levy et al, 2000;Thompson et al, 2001), and in South America (Rosales et al, 1998;Dal Zotto et al, 2006). Isolates of strain D have been described as being associated with relatively mild disease symptoms, and not efficiently transmitted by aphids (Levy et al, 2000, Wang et al, 2006IPPC, 2012).…”

Section: Strain Dideron (D)mentioning

confidence: 99%

Genetic diversity ofPlum pox virus: strains, disease and related challenges for control

James

Varga

Sanderson

2013

Canadian Journal of Plant Pathology

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Plum pox virus (PPV), a member of the genus Potyvirus, family Potyviridae, is a positive-sense single-stranded RNA virus. RNA viruses tend to be genetically diverse because the viral RNA-dependent RNA polymerase responsible for virus replication lacks proofreading capacity. Consequently, PPV is genetically diverse, and nine strains of the virus (D, M, EA, C, Rec, W, T, CR, and An) have been identified to date. At least two of these strains (Rec, T) are the direct products of recombination events, a contributor to genetic diversity and a common occurrence in the genus Potyvirus. The strains are genetically distinct and can be identified using phylogenetic analysis of the whole genome, the PIPO gene, the polymerase region, and the coat protein (CP) region. The unique genetic characteristics of each strain have facilitated the development of nucleic-acid-based and protein-based tools that allow strain identification. The strains are usually defined also by distinct biological properties that may include differences in host range, aphid transmissibility, disease symptomatology, and/or geographic distribution. There is genetic diversity within strains that affects these biological properties, and these differences may overlap with, and sometimes blur, the biological distinction between strains.Résumé: Le virus de la sharka du prunier (PPV), appartenant au genre Potyvirus de la famille des Potyviridae, est un virus à ARN simple brin à polarité positive. Les virus à ARN tendent à être différents génétiquement parce que l'ARN-polymérase sous la dépendance de l'ARN viral responsable de la réplication du virus ne possède pas la capacité de corriger les épreuves. En conséquence, le PPV est différent génétiquement et neuf souches du virus (D, M, EA, C, Ree, W, T, CR, et An) ont été identifiées à ce jour. Au moins deux de ces souches (Rec et T) sont les produits directs d'évènements de recombinaison, évènements qui contribuent à la diversité génétique et qui sont chose courante chez le genre Potyvirus. Les souches sont différentes génétiquement et elles peuvent être identifiées par analyse phylogénétique du génome entier, du gène PIPO, de la région de la polymérase et de la région de la capside. Les caractéristiques uniques propres à chaque souche ont facilité le développement d'outils basés sur les acides nucléiques et les protéines, outils qui permettent l'identification des souches. Celles-ci sont également habituellement définies par des propriétés biologiques qui peuvent comporter des différences quant à la gamme d'hôtes, à la transmissibilité par les pucerons, à la symptomatologie des maladies ou à la distribution géographique. Il y a des différences génétiques au sein des souches qui influencent ces propriétés biologiques et ces différences peuvent se superposer aux différences biologiques qui existent entre les souches, voire les brouiller à l'occasion.

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Section: Strain Dideron (D)mentioning

confidence: 99%

Genetic diversity ofPlum pox virus: strains, disease and related challenges for control

James

Varga

Sanderson

2013

Canadian Journal of Plant Pathology

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“…PCR is widely used to amplify specific nucleic acid regions; thus, there is a necessity to have primers of known nucleotide sequence. In the past, different primers have been proposed and used, mainly on the HCPro [387], C-terminal part of NIb [265,388], C-terminal part of CP [249,265,389], N-terminal part of CP [390] or 3´-noncoding region [272]. The request to known nucleotide sequence is absolutely necessary also in hybridization techniques, which are popular due to relative simplicity.…”

Section: Detection Of Ppvmentioning

confidence: 99%

Sharka: The Past, The Present and The Future

Sochor

Babula

Adam

et al. 2012

Viruses

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Members the Potyviridae family belong to a group of plant viruses that are causing devastating plant diseases with a significant impact on agronomy and economics. Plum pox virus (PPV), as a causative agent of sharka disease, is widely discussed. The understanding of the molecular biology of potyviruses including PPV and the function of individual proteins as products of genome expression are quite necessary for the proposal the new antiviral strategies. This review brings to view the members of Potyviridae family with respect to plum pox virus. The genome of potyviruses is discussed with respect to protein products of its expression and their function. Plum pox virus distribution, genome organization, transmission and biochemical changes in infected plants are introduced. In addition, techniques used in PPV detection are accentuated and discussed, especially with respect to new modern techniques of nucleic acids isolation, based on the nanotechnological approach. Finally, perspectives on the future of possibilities for nanotechnology application in PPV determination/identification are outlined.

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“…Originally reported in Bulgaria (Atanassov, 1932), the virus has progressively spread throughout Europe, the Mediterranean basin (Al Rwahnih et al, 2001), South America (Rosales et al, 1998), and most recently North America (Levy et al, 2000;Thompson et al, 2001). PPV is spread naturally by several aphid species in a non-persistent manner (Kunze and Krczal, 1971).…”

Section: Introductionmentioning

confidence: 99%

Stability of gene silencing-based resistance to Plum pox virus in transgenic plum (Prunus domestica L.) under field conditions

et al. 2004

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Plum pox virus (PPV) is one of the most devastating diseases of Prunus species. Since few sources of resistance to PPV have been identified, transgene-based resistance offers a complementary approach to developing PPV-resistant stone fruit cultivars. C5, a transgenic clone of Prunus domestica L., containing the PPV coat protein (CP) gene, has been described as highly resistant to PPV in greenhouse tests, displaying characteristics typical of post-transcriptional gene silencing (PTGS). We show in this report that C5 trees exposed to natural aphid vectors in the field remained uninfected after 4 years while susceptible transgenic and untransformed trees developed severe symptoms within the first year. C5 trees inoculated by chip budding showed only very mild symptoms and PPV could be detected in these trees by IC-RT-PCR. The PPV-CP transgene in C5 was specifically hyper-methylated with no detectable expression. These results indicate both stability and efficiency of PTGS-based PPV resistance in plum under field conditions.

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Molecular Characterization of Plum Pox Virus Isolated From Apricots, Plums and Peaches in Chile

Cited by 24 publications

References 0 publications

Genetic diversity ofPlum pox virus: strains, disease and related challenges for control

Genetic diversity ofPlum pox virus: strains, disease and related challenges for control

Sharka: The Past, The Present and The Future

Stability of gene silencing-based resistance to Plum pox virus in transgenic plum (Prunus domestica L.) under field conditions

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