Molecular characterization of porcine circovirus 2 circulating in Assam and Arunachal Pradesh of India

D’Silva, Ajai Lawrence; Bharali, Arpita; Buragohain, Lukumoni; Pathak, D. C.; Narayan, Raj; Batheja, Rahul; Kumar, Manoj; Gogoi, Sailen; Barman, Nagendra Nath; Dey, Sohini; Chellappa, Madhan Mohan

doi:10.1080/10495398.2021.1955700

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“…In the present study, the PCV2 isolate Assam-01 was isolated from a PCV2-PCR-positive pig tissue sample that is available in the repository of the Advanced Animal Disease Diagnosis and Management Consortium (ADMaC) Laboratory, College of Veterinary Science (Guwahati, India), and was received from a pig farm in Hekera, Kamrup, Assam, India, in 2017. The virus was isolated from pooled tissues (lung, liver, spleen, and kidney) from the same pig in PCV-free PK-15 cells by the method described earlier ( 9 ). The cells were harvested after the fourth passage, and DNA was extracted by using DNeasy blood and tissue kits (Qiagen).…”

Section: Announcementmentioning

confidence: 99%