Molecular Characterization of Postoperative Adhesions: The Adhesion Phenotype

Saed, Ghassan M.; Diamond, Michael P.

doi:10.1016/s1074-3804(05)60041-2

Cited by 100 publications

(150 citation statements)

References 56 publications

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“…Several phenotypic changes in adhesion fibroblasts as compared to normal peritoneal fibroblasts have been previously identified [6]: specifically, the marked increase in TGF-β1 and type I collagen expression in response to hypoxia [4]. Adhesion fibroblasts are myofibroblasts, defined as transiently activated fibroblasts exhibiting features intermediate between those of smooth muscle cells and fibroblasts, including the expression of α-SM actin [20,21] and a depleted antioxidant system [22].…”

Section: Discussionmentioning

confidence: 99%

“…Since we and others have previously reported a positive correlation between mRNA and protein levels for both TGFβ-1 and type I collagen, we will utilize the real-time RT/PCR to absolutely quantify mRNA copy numbers for each molecule [6,15,16]. We hypothesize that hypoxia exerts its effects through O 2 .− production.…”

Section: Introductionmentioning

confidence: 99%

“…Several molecular markers for the adhesion phenotype have been identified; fibronectin, type I collagen, vascular endothelial growth factor, transforming growth factor (TGF)-β1, alpha smooth muscle actin (α-SM actin), cyclooxygenase 2, tissue plasminogen activator/plasminogen activator inhibitor (tPA/PAI-1), and matrix metalloproteinase-1/tissue inhibitor of metalloproteinase (MMP-1/TIMP-1) ratios [3,6].…”

Section: Introductionmentioning

confidence: 99%

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Hypoxia-generated superoxide induces the development of the adhesion phenotype

Fletcher

Jiang

Diamond

et al. 2008

Free Radical Biology and Medicine

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Adhesion fibroblasts exhibit higher TGF-β1 and type I collagen expression as compared to normal peritoneal broblasts. Furthermore, exposure of normal peritoneal fibroblasts to hypoxia results in an irreversible increase in TGF-β1 and type I collagen. We postulated that the mechanism by which hypoxia induced the adhesion phenotype is through the production of superoxide either directly or through the formation of peroxynitrite. To test this hypothesis, normal peritoneal and adhesion fibroblasts were treated with superoxide dismutase (SOD), a superoxide scavenger, and xanthine/ xanthine oxidase, a superoxide-generating system, under normoxic and hypoxic conditions. Also, cells were treated with peroxynitrite. TGF-β1 and type I collagen expression was determined before and after all treatments using real-time RT/PCR. Hypoxia treatment resulted in a time-dependent increase in TGF-β1 and type I collagen mRNA levels in both normal peritoneal and adhesion fibroblasts. Similarly, treatment with xanthine oxidase, to endogenously generate superoxide, resulted in higher mRNA levels of TGF-β1 and type I collagen in both normal peritoneal and adhesion fibroblasts. In contrast, treatment with SOD, to scavenge endogenous superoxide, resulted in a decrease in TGF-β1 and type I collagen expression in adhesion fibroblasts to levels seen in normal peritoneal fibroblasts; no effect on the expression of these molecules was seen in normal peritoneal fibroblasts. Exposure to hypoxia in the presence of SOD had no effect on mRNA levels of TGF-β1 and type I collagen in either normal peritoneal or adhesion fibroblasts. Peroxynitrite treatment alone significantly induced both adhesion phenotype markers. In conclusion, hypoxia, through the production of superoxide, causes normal peritoneal fibroblasts to acquire the adhesion phenotype. Scavenging superoxide, even in the presence of hypoxia, prevented the development of the adhesion phenotype. These findings further support the central role of free radicals in the development of adhesions.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hypoxia-generated superoxide induces the development of the adhesion phenotype

Fletcher

Jiang

Diamond

et al. 2008

Free Radical Biology and Medicine

Self Cite

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show abstract

“…More importantly, it has been shown that conversion of these cells from the normal phenotype to the adhesion phenotype can be induced by hypoxia. 2 Compared with peritoneal fibroblasts, adhesion fibroblasts have a significant increase in basal mRNA levels of collagen I, fibronectin, matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase-1 (TIMP-1), transforming growth factor (TGF)-b1, cyclooxygenase-2 (COX-2), and interleukin (IL)-10.…”

Section: Ischemiamentioning

confidence: 99%

Prevention of adhesion in laparoscopic gynaecological surgery

Patel

Yadav

2016

Int J Reprod Contracept Obstet Gynecol

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“…Postsurgical adhesions arise from normal wound healing processes in tissues that are injured during surgery [10,20]. In addition to direct surgical injuries, unintentional tissue injuries that are caused by abrasive manipulation and desiccation of the organ surface can initiate an inflammatory process.…”

mentioning

confidence: 99%

Trehalose May Prevent Postsurgical Adhesions in a Rabbit Model of Hysterotomy

Fujino

Lee

Suzuki³

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. The adhesion-preventing effect of trehalose in visceral organs was evaluated in a rabbit model. All rabbits underwent a hysterotomy, during which mild abrasion and desiccation was induced on the surfaces of visceral organs. In the control group, the intestines were kept dry during hysterotomy. In the saline and trehalose groups, saline solution and 7% trehalose solution, respectively, were sprayed on the organ surfaces. Adhesion formation on organ surfaces on which abrasion and desiccation were induced declined significantly in the trehalose group compared with the other groups. In contrast, at the hysterotomy site, there was no significant difference in adhesions between groups, suggesting that trehalose prevents adhesions in organs that have developed minor inflammation by abrasion and desiccation.

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Molecular Characterization of Postoperative Adhesions: The Adhesion Phenotype

Cited by 100 publications

References 56 publications

Hypoxia-generated superoxide induces the development of the adhesion phenotype

Hypoxia-generated superoxide induces the development of the adhesion phenotype

Prevention of adhesion in laparoscopic gynaecological surgery

Trehalose May Prevent Postsurgical Adhesions in a Rabbit Model of Hysterotomy

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