Molecular Characterization of Pre-Core/Core and S Region of Hepatitis B Virus in Hemodialysis Patients With Occult Hepatitis B Infection

Rastegarvand, Nasrin; Makvandi, Manoochehr; Samarbafzadeh, Alireza; Rasti, Mojtaba; Neisi, Niloofar; Pouremamali, Amir; Teimoori, Ali; Shabani, Abdolnabi

doi:10.5812/jjm.23686

Cited by 10 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…20 Rastegarvand et al have declared 6/203(2.9%) HD patients were positive for HBV DNA, but negative for HBsAg test. 21 Ramezani et al demonstrated 2/100(2%) of HD patients were positive for anti-HBc test, negative for HBsAg test, among them HBV DNA was detected in 1(1%) case. 22 The spread of OBI is relied on endemicity of HBV infection, inadequate management of dialysis unit, familial HBV infection and contaminated blood transfusion.…”

Section: Discussionmentioning

confidence: 98%

Occult hepatitis B virus infection among hemodialysis patients

et al. 2018

Self Cite

View full text Add to dashboard Cite

Hepatitis B virus is a major public health impasse all over the world. Recently a new form of hepatitis B infection named Occult hepatitis B Infection (OBI) has appeared globally. The OBI is defined as the presence of HBV DNA in the liver and/or blood in the absence of detectable serum HBsAg with/without anti-HBc or anti-HBs. The prevalence of OBI has been reported in hemodialysis (HD) patients in different regions of the world. Thus, this study investigated the prevalence of OBI among HD patients. The cross-sectional study was carried out on 84 HD patients. These sera were checked for HBsAg, HBc-IgG assessment using Enzyme linked immunosorbent assay. The DNA was extracted from the sera samples and tested for HBVDNA detection using Nested Polymerase Chain Reaction (Nested PCR). The liver function tests including serum alanine aminotransferase and aspartate aminotransferase levels were carried out for all the HD individuals. 52/84(61.9%) of HD were males and 32/84 (38.1%) were females. The patient’s age ranged from 25 to 64 with a mean age of 52.4±15.2 years. HBsAg and HBc-IgG were detected in 1(1.1%) female. 2 (2.4%; a female and a male) patients were positive for HBsAg. 14/84 (16.7%; 6 female and 8 male) HD patients were positive for anti-HBc but negative for HBsAg, among them 4(28.6%; 2 female and 2 male) cases were positive for HBV DNA, indicating the presence of OBI in HD patients. Even distribution of OBI among the HD was found in 2(2.36%) male and 2(2.36%) female (P>.0.05). In the present study the moderate rate of 4.76% OBI has been observed in HD patients. The prevalence of seropositive OBI among the gender was 2(2.36%) male and 2(2.36%) female. The seronegative OBI have not been detected in the present study but requires further investigation. In this study the affliction of OBI in HD patients is not clear.

show abstract

Section: Discussionmentioning

confidence: 98%

Occult hepatitis B virus infection among hemodialysis patients

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…In Ahvaz, Neisi et al[ 109 ] detected HBV DNA in 10/250 (4%) of HD patients, who were negative for HBsAg but positive for anti-HBc. Also in Ahvaz, Rastegarvand et a [ 110 ] isolated HBV DNA in 6/216 (2.9%) HD patients, who were negative for HBsAg but positive for anti-HBc. The prevalence of OBI in HD patients was also reported in Spain (58%)[ 111 ], Egypt (26.9%)[ 24 ], United Kingdom (2.2%)[ 101 ], Greece (20.4%)[ 112 ] and Italy (0%)[ 113 ].…”

Section: Obi and Heamodialysismentioning

confidence: 99%

Update on occult hepatitis B virus infection

Makvandi¹

2016

WJG

Self Cite

157

147

View full text Add to dashboard Cite

The event of mutations in the surface antigen gene of hepatitis B virus (HBV) results in undetectable hepatitis B surface antigen with positive/negative anti-hepatitis B core (anti-HBc) antibody status in serum and this phenomenon is named occult hepatitis B infection (OBI). The presence of anti-HBc antibody in serum is an important key for OBI tracking, although about 20% of OBI cases are negative for anti-HBc antibody. The diagnosis of OBI is mainly based on polymerase chain reaction (PCR) and real-time PCR assays. However, real-time PCR is a more reliable method than PCR. OBI is a great issue for the public health problem and a challenge for the clinical entity worldwide. The persistence of OBI may lead to the development of cirrhosis and hepatocellular carcinoma. With regard to OBI complications, the screening of HBV DNA by the highly sensitive molecular means should be implemented for: (1) patients with a previous history of chronic or acute HBV infection; (2) patients co-infected with hepatitis C virus/human immunodeficiency virus; (3) patients undergoing chemotherapy or anti-CD20 therapy; (4) recipients of organ transplant; (5) blood donors; (6) organ transplant donors; (7) thalassemia and hemophilia patients; (8) health care workers; (9) patients with liver related disease (cryptogenic); (10) hemodialysis patients; (11) patients undergoing lamivudine or interferon therapy; and (12) children in time of HBV vaccination especially in highly endemic areas of HBV. Active HBV vaccination should be implemented for the close relatives of patients who are negative for OBI markers. Thus, the goal of this review is to evaluate the rate of OBI with a focus on status of high risk groups in different regions of the world.

show abstract

“…Similar to our study, the prevalence of OBI in seronegative individuals was higher than in seropositive individuals. Rastegarvand et al found that 2.3% of hemodialysis patients had OBI (20). In another study led by Neisi et al, the prevalence of OBI was determined to be 4% (21).…”

Section: Resultsmentioning

confidence: 99%

Prevalence of Occult Hepatitis B Virus Infection in Hemodialysis Patients Using Nested PCR

et al. 2020

View full text Add to dashboard Cite

Background: Occult hepatitis B infection (OBI) is defined as the lack of detectable HBsAg in serum, despite the presence of intrahepatic viral DNA, and low levels of covalently closed circular DNA (cccDNA). Since the hemodialysis patients are at a greater disadvantage if they are a carrier of Hep B, as it can lead to OBI this study was designed to determine the prevalence of OBI in hemodialysis patients residing in Zanjan, Iran. Methods: We conducted an anti-HBc test (ELISA) on 166 HBsAg negative hemodialysis patient samples. OBI was evaluated using seropositive (anti-HBc and/or anti-HBs) and seronegative (anti-HBc and anti-HBs) using nested PCR. Results: Out of the total hemodialysis patients sampled, the study consisted of 58.4% male and 41.6% female participants. The age of the study group ranged from 58.89±15.49, and had received approximately 28.27±27.43 years of dialysis. Additionally, 5.4% of patients had a history of blood transfusions, while 58.4% were vaccinated against the hepatitis B virus (HBV). Moreover, 23.5% patients were anti-HBc positive, while 76.5% patients tested negative. Lastly, 66.3% of the patients were positive for anti-HBs, whereas 33.7% were negative for anti-HBs. Overall, the study revealed that the prevalence of OBI was 6%, and HBV DNA was detected in 2.1% of individuals who were vaccinated against hepatitis B (p < 0.01). Conclusions: Though no significant difference between the prevalence of OBI to the patients' age, sex, duration of dialysis, or history of blood transfusion was identified, however, a strong correlation between the prevalence of OBI to HBV vaccination was found.

show abstract

Molecular Characterization of Pre-Core/Core and S Region of Hepatitis B Virus in Hemodialysis Patients With Occult Hepatitis B Infection

Cited by 10 publications

References 33 publications

Occult hepatitis B virus infection among hemodialysis patients

Occult hepatitis B virus infection among hemodialysis patients

Update on occult hepatitis B virus infection

Prevalence of Occult Hepatitis B Virus Infection in Hemodialysis Patients Using Nested PCR

Contact Info

Product

Resources

About