Molecular characterization of Rab7 and its involvement in innate immunity in red swamp crayfish Procambarus clarkii

Zhu, Lei; Yuan, Gaoliang; Wang, Xinru; Zhao, Tong; Hou, Libo; Li, Chen; Jiang, Xiaolong; Zhang, Jie; Zhao, Xianliang; Pei, Chao; Li, Li; Kong, Xianghui

doi:10.1016/j.fsi.2022.06.013

Cited by 10 publications

(2 citation statements)

References 34 publications

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“…NRF2 and STAT3 are key regulators of cellular antioxidant and immune responses. Our results suggested that rab7 may be essential for maintaining the antioxidant and immune balance, as supported by other studies [ 46 , 47 , 48 , 49 , 50 ]. Most importantly, we predicted multiple FOXO3 transcription factor binding sites in the rab7 promoter region ( Figure 2 ), confirming our initial hypothesis.…”

Section: Discussionsupporting

confidence: 91%

FOXO3/Rab7-Mediated Lipophagy and Its Role in Zn-Induced Lipid Metabolism in Yellow Catfish (Pelteobagrus fulvidraco)

Xiao,

Chen,

Zhang

et al. 2024

Genes

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Lipophagy is a selective autophagy that regulates lipid metabolism and reduces hepatic lipid deposition. However, the underlying mechanism has not been understood in fish. In this study, we used micronutrient zinc (Zn) as a regulator of autophagy and lipid metabolism and found that Ras-related protein 7 (rab7) was involved in Zn-induced lipophagy in hepatocytes of yellow catfish Pelteobagrus pelteobagrus. We then characterized the rab7 promoter and identified binding sites for a series of transcription factors, including Forkhead box O3 (FOXO3). Site mutation experiments showed that the −1358/−1369 bp FOXO3 binding site was responsible for Zn-induced transcriptional activation of rab7. Further studies showed that inhibition of rab7 significantly inhibited Zn-induced lipid degradation by lipophagy. Moreover, rab7 inhibitor also mitigated the Zn-induced increase of cpt1α and acadm expression. Our results suggested that Zn exerts its lipid-lowering effect partly through rab7-mediated lipophagy and FA β-oxidation in hepatocytes. Overall, our findings provide novel insights into the FOXO3/rab7 axis in lipophagy regulation and enhance the understanding of lipid metabolism by micronutrient Zn, which may help to reduce excessive lipid accumulation in fish.

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Section: Discussionsupporting

confidence: 91%

FOXO3/Rab7-Mediated Lipophagy and Its Role in Zn-Induced Lipid Metabolism in Yellow Catfish (Pelteobagrus fulvidraco)

Xiao,

Chen,

Zhang

et al. 2024

Genes

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“…During the six-day experiment, the number of crayfish deaths in each group (=12) was recorded daily, and the survival rate was calculated as the number of surviving crayfish divided by the initial number of crayfish [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Lipopolysaccharide and Deoxynivalenol on the Survival, Antioxidant and Immune Response, and Histopathology of Crayfish (Procambarus clarkii)

Wen

Xiang

et al. 2023

Toxins

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Bacterial lipopolysaccharide (LPS) in the aquatic environment has been reported to cause diseases in red swamp crayfish (Procambarus clarkii). In addition, deoxynivalenol (DON) is one of the primary mycotoxins found in aquaculture. However, the potential synergistic toxic effects of LPS and DON on crayfish are yet to be fully elucidated. In this study, crayfish were exposed to LPS (1 mg kg−1), DON (3 mg kg−1), and their combination (1 mg kg−1 LPS + 3 mg kg−1 DON, L+D) for a duration of six days. Co-exposure to LPS and DON exhibited the lowest survival rate compared to the control or individual treatments with LPS or DON alone. In the initial stage of the experiment, the combined treatment of LPS and DON showed a more pronounced up-regulation of antioxidant and immune-related enzymes in the sera compared to the other treatment groups, with a fold change ranging from 1.3 to 15. In addition, the (L+D) treatment group showed a down-regulation of immune-related genes, as well as Toll pathway-related genes in the hepatopancreas compared to LPS or DON. Moreover, the (L+D) treatment group demonstrated a 100% incidence of histopathological changes in the hepatopancreas, which were significantly more severe compared to the other three groups. In conclusion, our study provides physiological and histopathological evidence that the co-exposure to LPS and DON exerted synergistic toxic effects on crayfish. The observed effects could potentially hinder the development of the crayfish aquaculture industry in China.

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Ran in Procambarus clarkii: molecular characterization and immune function

Gu,

Zhao,

Wang

et al. 2024

Aquacult Int

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Molecular characterization of Rab7 and its involvement in innate immunity in red swamp crayfish Procambarus clarkii

Cited by 10 publications

References 34 publications

FOXO3/Rab7-Mediated Lipophagy and Its Role in Zn-Induced Lipid Metabolism in Yellow Catfish (Pelteobagrus fulvidraco)

FOXO3/Rab7-Mediated Lipophagy and Its Role in Zn-Induced Lipid Metabolism in Yellow Catfish (Pelteobagrus fulvidraco)

Effects of Lipopolysaccharide and Deoxynivalenol on the Survival, Antioxidant and Immune Response, and Histopathology of Crayfish (Procambarus clarkii)

Ran in Procambarus clarkii: molecular characterization and immune function

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