Molecular Characterization of Recently Classified Newcastle Disease Virus Genotype VII.1.1 Isolated from Egypt

doi:10.47278/journal.ijvs/2021.097b

Cited by 4 publications

(3 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The challenge virus was characterized by sequencing as velogenic NDV (vNDV) genotype VII1.1 designated as "NDV-CHICKEN-EGY-ALEX-NRC-2020" with an accession number of (MW580389) on GenBank (Ahmed et al, 2022a). The challenge with vNDV was carried out at 21 days of age.…”

Section: Challenge Viruses Nd Virusmentioning

confidence: 99%

The Effectiveness of Immunostimulants on NDV Genotype VII and IBDV Infection in Broiler Chickens

Ahmed¹,

Amer²,

El-Bayoumi³

et al. 2023

AAVS

View full text Add to dashboard Cite

The present study was conducted to investigate the pathogeneicity of Newcastle Disease Virus (NDV) genotype VII.1.1 "NDV-CHICKEN-EGY-ALEX-NRC-2020" strain in combination with Infectious Bursal Disease Virus (IBDV) "IBDV/Egypt/Qalubia/17" and/or some commercial immunostimulants (Lector ® and Orego ® ) in commercial broiler chickens. The pathogenicity studies parameters included the effects on protection, growth performance and clinico-pathological changes. The results indicated that, immunostimulants can keep maternal immunity longer where lector ® was the best; the decline in HI titers also indicates that bird groups not contract ND natural infection. The results of performance parameters after immunostimulants administration and challenged with IBDV at 14-days of age and NDV GenotypeVII.1.1 at 21-days of age in broiler chickens proved that administration of Lector ® and Orego ® solution had positive effect on average body weight gain and feed conversion rates than control non medicated groups; where the Lector medicated groups showed higher rates of average weight followed by Orego ® medicated groups. Regarding signs and mortality in infected groups IBD signs varied from mild in lector ® , moderate in Orego ® while sever signs were in non-medicated group. The mortalities began at 5 th day post-challenge (pch) in non-medicated groups with total 10-20%; while the mortalities in NDV began at 3 rd day pch in group non-medicated with 50% to reach 100% mortality rate in 7 th pch. At necropsy both IBD and NDV infection lesions were found in dead birds of all challenged groups. Chicken groups challenged by IBDV or NDV after treatment with Lector showed milder histopathological changes than Orego solution and non-treated groups showed the most sever lesions. Chicken groups challenged with both virulent IBDV and NDV either treatment with Lector ® or Orego ® solution or non-treated showed no marked difference in histopathological lesions. The results of total and differential leucocytes indicated that lector had a great high effect in WBCs levels specially lymphocytes and monocytes. In conclusion the used immunostimulants are of low value against IBD and ND virulent viruses challenge. In conclusion, is recommended to apply a strict hygienic measures and suitable vaccination programmes to protect chickens against both IBD and NDV infection as the current control and prevention regimes, including vaccines and vaccination protocol are not adequate against either single or mixed infection infections with IBD and/or ND.

show abstract

Section: Challenge Viruses Nd Virusmentioning

confidence: 99%

The Effectiveness of Immunostimulants on NDV Genotype VII and IBDV Infection in Broiler Chickens

Ahmed¹,

Amer²,

El-Bayoumi³

et al. 2023

AAVS

View full text Add to dashboard Cite

show abstract

“…Numerous waves of NDV outbreaks have been identified in Egypt in both vaccinated and unvaccinated poultry flocks (Osman et al, 2014;Awad et al, 2015;AbdEl-Aziz et al, 2016;Ahmed et al, 2017;Megahed et al, 2018;Sedeik et al, 2019;Shakal et al, 2020;. The sub-genotype VII.1.1 was the predominant strain that caused repeated outbreaks in Egypt in both acute and sub-acute forms despite the widespread application of various vaccine programs in poultry populations, particularly in recent years (Ahmed et al, 2022;Moharam et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Preliminary Evidence on Passage Attenuation of Orthoavulavirus javaense Genotype VII.1.1 and Its Potency as a Living Vaccine Candidate

El-Dabae,

Ibrahim,

Sadek

et al. 2024

AAVS

View full text Add to dashboard Cite

Newcastle disease (ND) is one of the most significant poultry viral diseases threatening the poultry industry worldwide. Despite mass vaccination against ND, genotype VII.1.1 epidemics in vaccinated and non-vaccinated flocks are still recorded, indicating an urgent need to generate a new vaccine candidate. In this study, the live Orthoavulavirus javaense (AOAV-1) genotype VII.1.1 strain (CH-EGY-GIZA-VVTNRC-2021) (Genbank accession number MW603772) was attenuated by combining passaging with limiting 10-fold dilutions in the allantoic cavity of embryonated hens eggs for 20 passages. The attenuated virus was used to vaccinate 20 SPF chicks and its protective efficacy was checked. Intracerebral pathogenicity index (ICPI) of the attenuated strain was 0.2, the mean death time (MDT) was 120 hours and infectvity titer was 7 log10 EID50/ml compared to 1.97 ICPI, 36 hours MDT, and 9.5 log10 EID50/ml of the original strain. The attenuated AOAV-1 was protective in vaccinated chicks by means of HI antibody response and protection from heterologus virulent challenge while showing little to no residual pathogencity. Histopathological inspection showed less severe pathological lesions in vaccinated challenged chickens than in nonvaccinated challenged chickens. Quantitation of viral loads following challenge indicated reduction of viral loads shed from birds vaccinated with the attenuated AOAV-1 virus. These preliminary insights collectively underline a new hope for the attenuation of live AOAV-1 genotype VII.1.1 as a vaccine candidate that can be used to control ND outbreaks in Egyptian poultry.

show abstract

“…Bacteria ( Mycobacterium tuberculosis , coliform bacillary), ectoparasites and endoparasites, fungal pathogens, and eleven virus species can spread diseases horizontally and vertically in poultry ( 8 , 9 ). One of the main reasons for financial losses in the global chicken industry is viral outbreaks ( 10 , 11 ). Zootechnical performances, including body weight gain, feed conversion ratio, feed intake, and the quality of the eggs and meat produced, are significantly impacted by viral outbreaks in the poultry industry ( 12 , 13 ).…”

Section: Introductionmentioning

confidence: 99%

Phytochemicals: a promising approach to control infectious bursal disease

Tahir,

Alsayeqh

2024

Front. Vet. Sci.

View full text Add to dashboard Cite

Infectious bursal disease (IBD) is one of the dangerous diseases of poultry that affects the bursa of Fabricius, which is an important organ of the bird’s immune system. IBD virus is resistant to many drugs, making its control difficult. Vaccination of IBD is in practice for a long time worldwide to control IBD, but secondary issues like vaccine failure and lower efficacy lead to their reduced use in the field. Multiple medicines are currently used, but the phytochemicals have emerged as promising agents for controlling IBD. The drugs to be developed should possess direct antiviral properties by targeting viral entry mechanisms, enhancing the host immune response, and inhibiting viral protein synthesis. Phytochemicals have potential to contribute to food security by minimizing the possibility of disease outbreaks and ensuring that consumers worldwide obtain healthy poultry products. It has been now claimed that direct and indirect activities of phytochemicals can be effective in the control of IBDV. Although available evidence suggest that the phytochemicals can contribute in controlling occurrence IBDV, there is a definite need of focused studies to gain more insight and develop rational strategies for their practical use. This review highlights the disease caused by IBDV, inhibition of viral replication, boosting the immune system, disruption of viral membrane, and important phytochemicals showing antiviral activities against IBDV.

show abstract

Molecular Characterization of Recently Classified Newcastle Disease Virus Genotype VII.1.1 Isolated from Egypt

Cited by 4 publications

References 37 publications

The Effectiveness of Immunostimulants on NDV Genotype VII and IBDV Infection in Broiler Chickens

The Effectiveness of Immunostimulants on NDV Genotype VII and IBDV Infection in Broiler Chickens

Preliminary Evidence on Passage Attenuation of Orthoavulavirus javaense Genotype VII.1.1 and Its Potency as a Living Vaccine Candidate

Phytochemicals: a promising approach to control infectious bursal disease

Contact Info

Product

Resources

About