Molecular Characterization of Resistance to Fusarium Head Blight in U.S. Soft Red Winter Wheat Breeding Line VA00W‐38

Christopher, M.; Griffey, Carl A.; Hall, M. D.; Gundrum, P. G.; Brooks, W. S.

doi:10.2135/cropsci2012.03.0144

Cited by 27 publications

(36 citation statements)

References 52 publications

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“…The most aggressive F. graminearum isolates from VA and MO, respectively, were used for spray inoculations (1 × 10 4 conidia spores per mL) in the field FHB nurseries at flowering time and for point inoculations (5 × 10 4 conidia spores per mL) of middle florets in greenhouse experiments. In field experiments, data for FHB variables including INC, SEV, IND, FDK and DON were collected following methods of Liu et al (2012a, b). The data collected from greenhouse followed the procedure by Liu et al (2007).…”

Section: Methodsmentioning

confidence: 99%

“…Among winter wheat sources for which resistance QTL have been mapped, most are of European descent [e.g., Sincron and F201R from Romania (Ittu et al 2000; Shen et al 2003), Renan from France (Gervais et al 2003), and Dream, Ritmo, and GS16-92 from Germany (Schmolke et al 2005; Klahr et al 2007; Schmolke et al 2008)]; however, they are not adapted to most of the winter wheat production areas of the US. Currently, only a few soft red winter (SRW) wheat sources from the US including Ernie, Goldfield, Freedom, IL 94-1653, and VA00W-38 (Liu et al 2005, 2007; Abate et al 2008; Gilsinger et al 2005; Gupta et al 2001; Bonin and Kolb 2009; Liu et al 2012b) have been genetically characterized for FHB resistance either in greenhouse or field conditions.…”

Section: Introductionmentioning

confidence: 99%

“…However, assessment of FHB resistance expressed under field conditions is more comprehensive and provides information on the different types and overall level of FHB resistance. Many FHB field experiments have been conducted using grain spawn and/or conidia (asexual spores) spray inoculations in mist-irrigated nurseries (Buerstmayr et al 2009; Liu et al 2012b). Assessments made in these studies have included FHB incidence (INC) and severity (SEV) with subsequent analysis of FHB index (IND), Fusarium damaged kernels (FDK), and concentrations of deoxynivalenol (DON).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of field resistance to Fusarium head blight in two US soft red winter wheat cultivars

et al. 2013

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In the soft red winter wheat (Triticum aestivum L.) regions of the US, Fusarium head blight (FHB, caused by Fusarium spp.) resistance derived from locally adapted germplasm has been used predominantly. Two soft red winter wheat cultivars, Massey and Ernie, have moderate resistance to FHB. Mapping populations derived from Becker/Massey (B/M) and Ernie/MO 94-317 (E/MO) were evaluated for FHB resistance and other traits in multiple environments. Eight QTL in B/M and five QTL in E/MO were associated with FHB variables including incidence, severity (SEV), index (IND), Fusarium damaged kernels (FDK), deoxynivalenol (DON), and morphological traits flowering time and plant height. Four QTL were common to both populations. Three of them were located at or near known genes: Ppd-D1 on chromosome 2DS, Rht-B1 on 4BS, and Rht-D1 on 4DS. Alleles for dwarf plant height (Rht-B1b and Rht-D1b) and photoperiod insensitivity (Ppd-D1a) had pleiotropic effects in reducing height and increasing FHB susceptibility. The other QTL detected for FHB variables were on 3BL in both populations, 1AS, 1DS, 2BL, and 4DL in B/M, and 5AL (B1) and 6AL in E/MO. The additive effects of FHB variables ranged from 0.4 mg kg−1 of DON to 6.2 % for greenhouse (GH) SEV in B/M and ranged from 0.3 mg kg−1 of DON to 8.3 % for GH SEV in E/MO. The 4DS QTL had epistasis with Ppd-D1, Qdon.umc-6AL, and Qht.umc-4BS, and additive × additive × environment interactions with the 4BS QTL for SEV, IND, and FDK in E/MO. Marker-assisted selection might be used to enhance FHB resistance through selection of favorable alleles of significant QTL, taking into account genotypes at Rht-B1b, Rht-D1a and Ppd-D1a.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-013-2149-y) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of field resistance to Fusarium head blight in two US soft red winter wheat cultivars

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“…A few major QTL have been found for FHB resistance including Fhb1 on chromosome 3B and Fhb2 on chromosome 6B that are derived from Chinese sources (Ghavami et al, 2011). In addition, QTL for FHB resistance have been found in two SRWW varieties: QTL were identified on chromosomes 1BL, 2D, and 7A in VA00W‐38 (Liu et al, 2012) and on 2B, 3B, 4BL, and 5A in ‘Ernie’ (Liu et al, 2007). Quantitative trait loci for FHB resistance have also been found in other types of wheat populations on chromosomes 1B, 1D, 2B, 2D, 3A, 3B, 4BL, 4D, 5A, and 7A with the 4D QTL likely being the dwarfing allele Rht‐D1b (Holzapfel et al, 2008; Liu et al, 2007; Miedaner et al, 2011).…”

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Identifying Quantitative Trait Loci for Economic Traits in an Elite Soft Red Winter Wheat Population

Hoffstetter¹,

Cabrera²,

Sneller³

2016

Crop Science

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Genotyping‐by‐sequencing (GBS), reduces genotyping costs and allows breeders to genotype large populations with thousands of markers. Genome‐wide association studies with GBS markers can be used to identify quantitative trait loci (QTL) for important traits in elite populations of soft red winter wheat (SRWW; Triticum aestivum L.). Our objective was to identify potential QTL for grain yield (GY), Fusarium head blight resistance (FHB), flour yield (FY), and softness equivalence (SE) in a set of 470 elite SWRR wheat lines that were genotyped with 33,169 GBS markers and phenotyped in multiple environments. For all traits, we found lines that were phenotypically superior to the elite checks. We identified four FHB QTL, nine QTL for quality, and 14 QTL for with R2 values ranging from 1.6 to 3.5%. The QTL with the largest effect for FHB resistance reduced disease by 1.76%. For quality, the largest‐effect QTL increased FY and SE by 0.37 and 0.67%, respectively. For GY the QTL with the largest effect in Wooster, OH, increased GY by 129.6 kg ha−1, for northwest Ohio, the largest‐effect QTL increased GY by 67.2 kg ha−1, and the largest‐effect QTL for GY over all environments increased GY by 48.8 kg ha−1. While marker‐assisted selection (MAS) for these QTL could be used to improve these traits, the preponderance of genetic variation appeared to be controlled by genes with small effect, suggesting that MAS should be used as a supplement to genomic selection.

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“…Additionally, more than 50 peer reviewed publications have presented results on more than 250 QTL associated with several FHB resistance traits (reviewed by Buerstmayr et al, 2009;Liu et al, 2009;Löffler et al, 2009). Genetic studies involving U.S. soft red winter wheat sources have included the cultivars Ernie (Liu et al, 2005(Liu et al, , 2007Abate et al, 2008;Liu et al, 2013), Massey (Liu et al, 2013), Goldfield (Gilsinger et al, 2005), Freedom (Gupta et al, 2001), IL 94-1653 (Bonin and Kolb, 2009), and VA00W-38 (Liu et al, 2012). The demand for identification and mapping of resistance in native, well-adapted sources has therefore increased.…”

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Mapping of Fusarium Head Blight Resistance Quantitative Trait Loci in Winter Wheat Cultivar NC‐Neuse

et al. 2016

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Fusarium head blight (FHB), primarily caused by Fusarium graminearum, can significantly reduce the grain quality of wheat (Triticum aestivum L.) due to mycotoxin contamination. The objective of this study was to identify quantitative trait loci (QTL) for FHB resistance in the moderately resistant soft red winter wheat cultivar NC‐Neuse. A total of 170 recombinant inbred lines (RILs) from a cross between NC‐Neuse and the susceptible cultivar AGS 2000 were evaluated in inoculated, mist‐irrigated field nurseries. The lines were evaluated for FHB incidence (INC), severity (SEV), Fusarium‐damaged kernels (FDK), and deoxynivalenol (DON) content in seven environments between 2011 and 2014. A 3,419 cM linkage map was developed based on 1839 simple sequence repeat (SSR), diversity array technology (DArT), and single nucleotide polymorphism (SNP) markers. Seven FHB resistance QTL on chromosomes 1A, 1B, 1D, 2A, 4A, 5B, and 6A were mapped. The QTL alleles conferring resistance on 1A, 1B, 2A, 4A, and 6A originated from NC‐Neuse, while the alleles associated with resistance on 1D and 5B originated from AGS 2000. Quantitative trait loci effects ranged from 9 to 12% for INC, from 6 to 11% for SEV, from 8 to 20% for FDK, and from 6 to 18% for DON. The QTL on 5B co‐localized with the Vrn‐B1 locus. Kompetitive Allele‐Specific PCR (KASP) assays were developed for each NC‐Neuse QTL region. A preliminary test using these assays on recent Uniform Southern Winter Wheat Nursery (USWWN) entries indicated Qfhb.nc‐1A, Qfhb.nc‐1B, and Qfhb.nc‐6A as likely the best candidates for use in marker‐assisted selection.

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Molecular Characterization of Resistance to Fusarium Head Blight in U.S. Soft Red Winter Wheat Breeding Line VA00W‐38

Cited by 27 publications

References 52 publications

Molecular characterization of field resistance to Fusarium head blight in two US soft red winter wheat cultivars

Molecular characterization of field resistance to Fusarium head blight in two US soft red winter wheat cultivars

Identifying Quantitative Trait Loci for Economic Traits in an Elite Soft Red Winter Wheat Population

Mapping of Fusarium Head Blight Resistance Quantitative Trait Loci in Winter Wheat Cultivar NC‐Neuse

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