Molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients

Pereira, Luciane Aparecida; Ferreira, Caroline do Nascimento; Turchetto, Giovana D.; Nogueira, Meri Bordignon; Vidal, Luíne Rosele Renaud; Cruz, Cristina Rodrigues; Debur, Maria C.; Almeida, Sérgio Monteiro de; Raboni, Sônia Mara

doi:10.1016/j.jped.2012.11.002

Cited by 4 publications

(5 citation statements)

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“…Gastroenteritis and diarrhea caused by rotavirus infections pose a significant threat to especially the health of newborns and immunosuppressed in low- and middle income countries [54, 55]. The rotavirus vaccine for infants recommended by WHO in 2013 has the potential to counter that danger but until a herd effect is reached [56], RV remains one of the most relevant waterborne enteric viruses.…”

Section: Discussionmentioning

confidence: 99%

From Lab to Lake – Evaluation of Current Molecular Methods for the Detection of Infectious Enteric Viruses in Complex Water Matrices in an Urban Area

et al. 2016

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Quantitative PCR methods are commonly used to monitor enteric viruses in the aquatic environment because of their high sensitivity, short reaction times and relatively low operational cost. However, conclusions for public health drawn from results of such molecular techniques are limited due to their inability to determine viral infectivity. Ethidium monoazide (EMA) and propidium monoazide (PMA) are capable to penetrate the damaged or compromised capsid of the inactivated viruses and bind to the viral nucleic acids. We assessed whether dye treatment is a suitable approach to improve the ability of qPCR to distinguish between infectious and non-infectious human adenovirus, enterovirus and rotavirus A in surface water of an urban river and sewage before and after UV disinfection. Like the gold standard of cell culture assays, pretreatment EMA-/PMA-qPCR succeeded in removing false positive results which would lead to an overestimation of the viral load if only qPCR of the environmental samples was considered. A dye pretreatment could therefore provide a rapid and relatively inexpensive tool to improve the efficacy of molecular quantification methods in regards to viral infectivity.

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Section: Discussionmentioning

confidence: 99%

From Lab to Lake – Evaluation of Current Molecular Methods for the Detection of Infectious Enteric Viruses in Complex Water Matrices in an Urban Area

et al. 2016

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“…However, diarrhoea still represents a frequent cause of hospitalisation and, except for RVA, the role of other enteric viruses was not determined in our patients and their incidence was likely underestimated. Previously, we have carried out RV genotypic characterisation in all positive samples and G2 P[4] was the most prevalent genotype after the vaccine implementation in 2006 (Pereira et al 2013). …”

Section: Discussionmentioning

confidence: 99%

Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes

Raboni

Damasio

Ferreira

et al. 2014

Mem. Inst. Oswaldo Cruz

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Viral acute gastroenteritis (AG) is a significant cause of hospitalisation in children younger than five years. Group A rotavirus (RVA) is responsible for 30% of these cases. Following the introduction of RVA immunisation in Brazil in 2006, a decreased circulation of this virus has been observed. However, AG remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. We conducted a prospective study of paediatric patients hospitalised for AG. Stool samples were collected to investigate human adenovirus (HAdV), RVA, norovirus (NoV) and astrovirus (AstV). NoV typing was performed by nucleotide sequencing and phylogenetic analysis. From the 225 samples tested, 60 (26%) were positive for at least one viral agent. HAdV, NoV, RVA and AstV were detected in 16%, 8%, 6% and 0% of the samples, respectively. Mixed infections were found in nine patients: HAdV/RVA (5), HAdV/NoV (3) and HAdV/NoV/RVA (1). The frequency of fever and lymphocytosis was significantly higher in virus-infected patients. Phylogenetic analysis of NoV indicated that all of these viruses belonged to genotype GII.4. The significant frequency of these pathogens in patients with AG highlights the need to routinely implement laboratory investigations.

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“… 35 São Paulo 2006 - 2009 G9P[8] – dominant type in 2006 G2P[4] – dominant type in 2007 / 2008 / 2009 Cilli et al 11 São Paulo 2006 - 2008 In hospitalized children: G2P[4] - 15% (2006) / 70% (2007) / 100% (2008) Sáfadi et al 36 Belém 2008 - 2009 In hospitalized children: G2P[4] predominance Justino et al 37 Minas Gerais 2009 - 2010 G2P[4] predominance Dulgheroff et al. 38 Curitiba 2001 - 2008 In hospitalized patients: G2P[4] predominance after 2006 Pereira et al 39 18 Brazilian states 2005 - 2009 G9 – dominant type in 2005 (52%) G2P[4] – 49% (2006) / 66% (2007) / 85% (2008) / 37%(2009) Carvalho-Costa et al. 6 Australia 2007 - 2009 Increase in G2P[4] rates in most states where Rotarix® is in use Kirkwood et al 40 …”

Section: Resultsmentioning

confidence: 99%

Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil

Leite

Carmona

Carraro

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Rotavirus is the main global cause of severe childhood diarrhoea among children. In 2006, Rotarix® (G1P[8]) was introduced into Brazil’s National Immunization Program. The vaccine coverage rate was 84.4% in 2009. Evidences of increasing G2P[4] after 2006 opened up the discussion about the vaccine effectiveness to non-G1 strains. The aim of this study was to identify the circulating rotavirus genotypes in two Brazilian regions during 2009. A total of 223 positive samples by immunochromatography and latex agglutination assay from the Northeast (Bahia/Pernambuco States) and Southeast (São Paulo/Rio de Janeiro States) regions were included in the study. The samples were submitted to genotyping by nested-PCR according to VP7(G) and VP4(P) and 175 samples (78.5%) were able to be characterized. Considering the characterization of VP7, the G-types detected were G1, G2, and G4 in the Northeast, and G2, G3, G5, and G9 in the Southeast. Considering the characterization of VP4, the P-types detected were P[4], P[8], and P[6]/P[9] in the Northeast and the Southeast. The most frequent mixed types found were G2P[4]/G2P[NT](81.4%), G2P[6](5.2%), G1P[6](5.2%) in the Northeast, and G2P[4]/G2P[NT](78.8%), G2P[6](8.2%), G9P[8](4.7%) in the Southeast. Among immunized individuals whose age ranged from 0-4 years, the G2P[4]/G2P[NT] genotype was identified in 91,0% of cases, and among non-immunized individuals of the same age, the G2P[4]/G2P[NT] genotype was identified in 85.7% of the cases. In accordance with the high level of vaccine coverage, the data suggest that the circulation of G2P[4] in these regions had a considerable increase after the introduction of Rotarix®.

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Molecular characterization of rotavirus genotypes in immunosuppressed and non-immunosuppressed pediatric patients

Abstract: RVA infections can be associated with severe clinical manifestations, and the surveillance of genotypic variability of this virus is crucial to monitor the emergence of new strains and the impact of the immunization in these patients.

Cited by 4 publications

References 30 publications

From Lab to Lake – Evaluation of Current Molecular Methods for the Detection of Infectious Enteric Viruses in Complex Water Matrices in an Urban Area

From Lab to Lake – Evaluation of Current Molecular Methods for the Detection of Infectious Enteric Viruses in Complex Water Matrices in an Urban Area

Acute gastroenteritis and enteric viruses in hospitalised children in southern Brazil: aetiology, seasonality and clinical outcomes

Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil

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