Molecular characterization of Salmonella strains in individuals with acute diarrhea syndrome in the State of Sucre, Venezuela

Rodulfo, Hectorina; Donato, Marcos De; Luiggi, Jesús; Michelli, Elvia; Millán, Adriana; Michelli, Miriam

doi:10.1590/s0037-86822012000300010

Cited by 13 publications

(8 citation statements)

References 22 publications

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“…The mouth is the typical portal of entry for gastrointestinal pathogens, which are ingested alongside contaminated food and water (Rodulfo et al, 2012). Also, they are acquired via contact with diarrhoeic animals and their contaminated environments or with the faecal matter of a diarrhoeic person (Humphries and Linscott, 2015;Squire and Ryan, 2017).…”

Section: Introductionmentioning

confidence: 99%

Childhood diarrhoeal diseases in developing countries

Ugboko

Nwinyi

Oranusi

et al. 2020

Heliyon

148

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Diarrhoeal diseases collectively constitute a serious public health challenge globally, especially as the leading cause of death in children (after respiratory diseases). Childhood diarrhoea affecting children under the age of five accounts for approximately 63% of the global burden. Accurate and timely detection of the aetiology of these diseases is very crucial; but conventional methods, apart from being laborious and time-consuming, often fail to identify difficult-to-culture pathogens. The aetiological agent of an average of up to 40% of cases of diarrhoea cannot be identified. This review gives an overview of the recent trends in the epidemiology and treatment of diarrhoea and aims at highlighting the potentials of metagenomics technique as a diagnostic method for enteric infections.

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Section: Introductionmentioning

confidence: 99%

Childhood diarrhoeal diseases in developing countries

Ugboko

Nwinyi

Oranusi

et al. 2020

Heliyon

148

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“…The main reservoir of Salmonella enterica includes chickens, turkeys, ducks, parrots and coastal species. Salmonella infections are recognized as zoonotic infections transmitted through contaminated meat and processed poultry products [7,8].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial role of Lactobacillus species as potential probiotics against enteropathogenic bacteria in chickens

Ahmed

Vohra

Khan

et al. 2019

J Infect Dev Ctries

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Introduction: The emergence of antimicrobial resistance among bacterial community resulted in a ban on drugs as the growth promoter in poultry feed. This situation demands to explore alternatives as food supplements with health benefit to poultry. Therefore, probiotic microorganisms, which are considered as safe and possess various health benefits can be a choice. Present study was designed to explore the probiotic potential of the isolated lactobacillus species in chickens. Methodology: Out of 220 samples, 100 Lactobacillus species were isolated from various regions of chicken intestine. They were further characterized on the basis of morphology, staining and catalase test. Species-level identification was made by amplifying Lactobacillus specific 16S rRNA gene. Out of 100 isolates, 21 were selected for sequencing on the basis of band intensity. Results: Among 21 sequences, 16 were identified as L. paracasei (n = 6), L. salivarius (n = 3), L. johnsonii (n = 3), and L. agilis, L. fermentum, L. sakei, and L. curvatus (n = 1 each). These strains were found to be significantly acid-tolerant with 81.68 - 85.01% survival rate at pH 2)and bile-tolerant with 81.96 -84.65% survival rate at 0.3% bile. Except three; all strains showed salt tolerance to 2% and 4% NaCl. Among 21 Lactobacillus strains, 6 showed good antimicrobial activities against S. aureus, Salmonella Typhimurium and E. coli. Conclusion: Lactobacillus species with probiotic property can be used in poultry feed formulation for their health benefit to combat gastrointestinal infections.

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“…Since 2000, childhood mortality due to diarrhoea has diminished by 6.5% every year, but this trend requires an acceleration to reach the 2030 objectives. Diarrhoea infections are associated with acute gastroenteritis, one of the most common alimentary diseases; caused by the consumptions of contaminated water and food especially meat [5]. The prevalence rate in Nigeria is about 18.8%, one of the worst in sub-Saharan Africa and accounts for over about 16% of child-deaths and estimated 150,000 deaths chiefly among children less than five years of age which occurs annually due to this disease which is caused by poor sanitations and poor hygiene practices [2].…”

Section: Introductionmentioning

confidence: 99%

Screening for Antimicrobial Activities of Metanollic Extracts of Aloe vera and Hyptis suaveolens against Co-infections of Giardia lamblia and Salmonella among Diarrhoeagenic Children

Abdulrahman

Samaila

Panda

et al. 2019

SAJRM

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The antimicrobial and Phytochemicals activities of methanol extracts obtained from Aloe vera and Hyptis suaveolens plants were investigated individually and combined in an attempt to evaluate their medicinal potentials and efficacies on protozoan; Giardia lamblia and bacteria; Salmonella species as co-infections causing diarrhoea in under five populations in Bauchi State, Nigeria. The phytochemical screening revealed the presence of saponins, tannins, alkaloids, flavonoids, terpenoids, alkaloids, phenols. Antimicrobial activity was determined against Giardia lamblia and Salmonella species; anti-giardial activity, an in-vitro susceptibility assays method was performed and antibacterial activity was carried out by Kirby-Bauer method. The parasites mortality was determined by counting in hemocytometer under a light microscope and the zone of inhibition diameter produced against the bacteria were determined, expressed as mean ±SEM (Standard Error of Mean) and the differences between means were statistically analyzed and compared. The results obtained showed that methanol extracts of Aloe vera and Hyptis suaveolens singly used and in combinations had inhibitory effects on Giardia lamblia and Salmonella species. In all cases, the extractions, plants, concentrations and time were determinant factors for the anti-giardial and antibacterial activity. Anti-giardial activity was best recorded with extracts of Aloe vera which showed anti-giardial activity of (0.002±0.553), and activity was greater in combined Aloe vera and Hyptis suaveolens which showed anti-giardial activity of (0.002±0.679). Also, antibacterial activity of methanol extracts of these plants on Salmonella species, showed higher zone of inhibitions diameter with extracts of Aloe vera (0.895±20.17) and the efficacy becomes higher with the combined Aloe vera and Hyptis suaveolens which zone of inhibitions is (0.423±27.50).

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Molecular characterization of Salmonella strains in individuals with acute diarrhea syndrome in the State of Sucre, Venezuela

Cited by 13 publications

References 22 publications

Childhood diarrhoeal diseases in developing countries

Childhood diarrhoeal diseases in developing countries

Antimicrobial role of Lactobacillus species as potential probiotics against enteropathogenic bacteria in chickens

Screening for Antimicrobial Activities of Metanollic Extracts of Aloe vera and Hyptis suaveolens against Co-infections of Giardia lamblia and Salmonella among Diarrhoeagenic Children

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