Molecular Characterization of Slow-Growing Orange and Key Lime Anthracnose Strains of<i>Colletotrichum</i>from Citrus as<i>C. acutatum</i>

Brown, Averil E.; Sreenivasaprasad, S.; Timmer, L. W.

doi:10.1094/phyto-86-523

Cited by 145 publications

(94 citation statements)

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“…Overall, maximum growth rates of between 6.2 and 16.6 mm per day have been measured at optimum temperatures between 25 and 30oC (Adaskaveg and Hartin, 1997;Kuramae-Izioka et al, 1997;Lenné, 1978). Muniz et al (1997) recorded growth of C. gloeosporioides isolates from cashew on malt extract agar (MEA) and showed that maximum rates (7.7-14.8 mm/day) occurred from 25 to 30 o C. The isolates of C. acutatum, however, exhibited much slower growth, with a maximum at 25 o C (Adaskaveg and Hartin, 1997; Agostini et al, 1992;Brown et al, 1996;Kuramae-Izioka et al, 1997;Smith and Black, 1990). Since this distinction was provided by the molecular approaches like RAPDs and sequencing of ITS, Adaskaveg and Hartin (1997) suggested that temperature relationships could be combined with the mycelial growth rates to distinguish C. acutatum from C. gloeosporioides.…”

Section: Cultural Morphologymentioning

confidence: 99%

Colletotrichum isolates related to Anthracnose of cashew trees in Brazil: morphological and molecular description using LSU rDNA sequences

López

Lucas

2010

Braz. arch. biol. technol.

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Section: Cultural Morphologymentioning

confidence: 99%

Colletotrichum isolates related to Anthracnose of cashew trees in Brazil: morphological and molecular description using LSU rDNA sequences

López

Lucas

2010

Braz. arch. biol. technol.

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“…Dentre essas doenças, pode-se destacar a "Queda Prematura dos Frutos Cítricos" (QPFC), descrita pela primeira vez em Belize, em 1979, como sendo causada pelo fungo Colletotrichum gloeosporioides Penz (Fagan, 1979), que foi reclassifi cado por Brown et al (1996), como Colletotrichum acutatum Simmonds.…”

Section: Introductionunclassified

Controle alternativo de Colletotrichum acutatum agente causal da queda prematura dos frutos cítricos

Kupper

Bellotte²,

Goes

2009

Rev. Bras. Frutic.

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RESUMO -A queda prematura dos frutos cítricos (QPFC), causada por Colletotrichum acutatum, dados os grandes prejuízos que têm causado aos produtores, constitui-se numa doença de grande importância econômica. O controle da doença é feito predominantemente mediante uso de fungicidas, que eleva o custo de produção e afeta negativamente o meio ambiente. Diante disso, este trabalho teve por objetivo buscar um método alternativo de controle da QPFC, mediante o uso de agentes de biocontrole ou de biofertilizantes. ALTERNATIVE CONTROL OF Colletotrichum acutatum, CAUSAL AGENT OF POSTBLOOM FRUIT DROP OF CITRUSABSTRACT -Postbloom Fruit Drop of Citrus (PDF), caused by Colletotrichum acutatum, has caused losses to citrus producers and therefore it is economically very important. This disease is generally controlled by fungicide sprayings, which increase the production cost, and affects the environment. Thus, this work aimed at developing an alternative control method through the use of biocontrol agents, or biofertilizers. Different concentrations of biofertilizers (from two distinct sources and named Bio1 and Bio2), three strains of Bacillus subitlis (BCA-69, 72 and 77) and three isolates of Trichoderma spp. (BCA-14, 37 and 39) were tested, in vitro, alone or in combination, for the inhibitory capacity of conidia germination of C. acutatum. It was studied the production of thermo-stable metabolites by B. subtilis and their effect on the germination of C. acutatum conidia. Under in vivo conditions, 15 isolates of B. subtilis were tested for the ability to prevent C. acutatum infection on 'Tahiti' lime detached fl owers and in the fi eld, where two experiments were installed, for testing the BCA and biofertilizers in the control of the disease. It was found that the isolate BCA-72 (B. subtilis) and BCA-37 (T. pseudokoningii) were the most effi cient in inhibiting conidia germination. BCA-69 and BCA-77 were the most effective in producing anti-fungal substances, and in quantities suffi cient to inhibit the germination of C. acutatum. The mixture of four strains of Bacillus (BCA: 69, 72, 77 and AP3) presented the highest percentage of inhibition (73%). Regarding the use of biofertilizers (Bio1 and Bio2), it was observed that concentrations above 10% of the product, when combined with the strains of Trichoderma spp. decreased the pathogen germination. The experiments with detached fl owers showed that the fl owers where the BCA: 69, 76, 74 and 77 were applied presented 83, 92, 92 and 97% respectively, of the petals without symptoms of infection by C. acutatum. Field tests showed the potential of B. subtilis and biofertilizers in controlling the disease.

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“…(Jonhston & Jones, 1997), pêssego (Prunus persica (L.) Batsch.) (Adaskaveg & Hartin, 1997); citros (Brown et al, 1996;Goes & Kimati, 1997) e algumas solanáceas (Tozze Jr. et al, 2006) são infectadas por C. acutatum e C. gloeosporioides. No Brasil, entre as fruteiras tropicais, C. acutatum foi associado somente à goiabeira (Psidium guajava L.) (Peres et al, 2002), embora tenha sido descrito inicialmente em C. papaya por Simmonds (1965), na Austrália.…”

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Caracterização morfocultural e molecular de isolados de Colletotrichum gloeosporioides patogênicos ao mamoeiro

et al. 2007

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Vinte e nove culturas monospóricas de Colletotrichum, isoladas de frutos e pecíolos de mamoeiro (Carica papaya), foram caracterizadas quanto à morfologia dos conídios e apressórios, coloração e crescimento das colônias, sensibilidade ao benomyl, presença de setas e do teleomorfo, PCR com primers taxon-específicos e análise de PCR-RFLP da região ITS. Os 29 isolados foram identificados como C. gloeosporioides com base na morfologia dos conídios e apressórios, tendo a maioria dos isolados conídios cilíndricos e/ou obclavados e apressórios lobados ou fracamente lobados, em contraste com C. acutatum, isolado de morango (Fragaria x ananassa), que apresentou conídios fusiformes e apressórios circulares e lisos. Presença de setas e do teleomorfo, cor de colônia, sensibilidade ao benomyl e velocidade de crescimento variaram conforme o isolado e sofreram influência do meio de cultura usado. Todos os isolados de mamão e quatro de outras hospedeiras, manga (Mangifera indica), morango e maçã (Malus domestica), foram patogênicos a frutos de mamão cv. Sunrise Solo, mas com variabilidade em agressividade. PCR com o primer específico para C. gloeosporioides, CgInt, confirmou a identidade de apenas quatro isolados de mamão e dois isolados apresentaram reação positiva com o primer CaInt2, específico para C. acutatum. A maioria dos isolados de mamão (23) não reagiu com nenhum dos primers. Por outro lado, a análise de restrição da região ITS do rDNA, com RsaI, gerou perfis distintos entre C. gloeosporioides e C. acutatum e mostrou uniformidade entre os isolados de mamão.

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Molecular Characterization of Slow-Growing Orange and Key Lime Anthracnose Strains ofColletotrichumfrom Citrus asC. acutatum

Cited by 145 publications

References 0 publications

Colletotrichum isolates related to Anthracnose of cashew trees in Brazil: morphological and molecular description using LSU rDNA sequences

Colletotrichum isolates related to Anthracnose of cashew trees in Brazil: morphological and molecular description using LSU rDNA sequences

Controle alternativo de Colletotrichum acutatum agente causal da queda prematura dos frutos cítricos

Caracterização morfocultural e molecular de isolados de Colletotrichum gloeosporioides patogênicos ao mamoeiro

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