Molecular characterization of South and East Asian melon, Cucumis melo L., and the origin of Group Conomon var. makuwa and var. conomon revealed by RAPD analysis

Tanaka, Katsunori; Nishitani, Atsushi; Akashi, Yukari; Sakata, Yoshiteru; Nishida, Hidetaka; Yoshino, Hiromichi; Kato, Kenji

doi:10.1007/s10681-006-9259-4

Cited by 55 publications

(62 citation statements)

References 28 publications

(28 reference statements)

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“…conomon, of Asian origin (ROBINSON; DECKER-WALTERS, 1997), found in the accession BGMEL 109, has been reported to have agronomic characteristics such as low content of soluble solids, smooth, yellow skin, white pulp colour and small seeds (TANAKA et al, 2006). In contrast, Torres Filho et al (2009) identified significant characteristics for breeding programs in this botanical variety, such as high prolificacy and high firmness.…”

Section: Resultsmentioning

confidence: 99%

Botanical Identification and Genetic Diversity in Melons From Family Farming in the State of Maranhão

et al. 2017

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-The aim of this work was to perform botanical identification and to estimate genetic diversity in two sequential inbred generations (progenies S 1 and S 2 ) of melon accessions from traditional agriculture in the state of Maranhão, in order to generate useful information for commercial melon breeding. Two field experiments were carried out in a completely randomized block, using four replicates of 15 accessions from a first selfing cycle in 2013, and three replicates of 25 subaccessions (generation S 2 ) in 2014. Flower and fruit descriptors were measured to obtain quantitative and qualitative data, in addition to a systematized photographic documentation of fruit for visually comparing the progenies S 1 and S 2 . Distance matrices for quantitative and qualitative data were obtained and used to perform a joint analysis and UPGMA method. Large genetic diversity was found in the accessions analysed, since the presence of melon progenies was observed in the Cucumis melo ssp. agrestis, with its botanical varieties momordica and conomom, and of the Cucumis melo ssp. melo, with the botanical varieties cantalupensis and chandalak. Divergence analysis showed the formation of three groups in generation S 1 and four groups in S 2 . However, the groups were not separated either by subspecies or by botanical variety. Thus, in addition to the large genetic diversity among and within melon accessions from family farming in the state of Maranhão, the progenies presented a large introgression of traits of the different subspecies and their botanical varieties due to the reproductive system and seed management of these species.Keywords: Botanical variety. Cucumis melo L.. Endogamic progenies.CLASSIFICAÇÃO BOTÂNICA E DIVERGÊNCIA GENÉTICA EM MELÕES DA AGRICULTURA FAMILIAR MARANHESE RESUMO -O trabalho teve como objetivos realizar a classificação botânica e estimar a diversidade genética em duas gerações endogâmicas sequenciais (progênies S 1 e S 2 ) de acessos de melão da agricultura familiar do Maranhão, visando gerar informações úteis para o melhoramento do melão comercial. Foram conduzidos dois experimentos de campo em blocos casualizados completos, com quatro repetições e 15 acessos em 2013 e, três repetições e 25 subacessos em 2014. Para avaliação dos acessos foram aplicados descritores quantitativos e qualitativos de flor e fruto, além de uma documentação fotográfica sistematizada dos frutos com análise visual, comparando os frutos das gerações S 1 e S 2 . Foram obtidas as matrizes de distância nos dois tipos de descritores e se fez a análise conjunta e em seguida realizou-se o agrupamento pelo método UPGMA. Verificou-se que nos acessos analisados, existe uma grande diversidade genética, pois foram encontradas progênies de melão da subespécie agrestis e suas variedades botânicas momordica e conomom e da subespécie melo com as variedades botânicas cantalupensis e chandalak. A análise de divergência na geração S 1 mostrou a formação de três grupos e na geração S 2 foram formados quatro agrupamentos, entretanto os grup...

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Section: Resultsmentioning

confidence: 99%

Botanical Identification and Genetic Diversity in Melons From Family Farming in the State of Maranhão

et al. 2017

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“…Eighteen random primers (12-mer; Bex, Japan), which were selected for their ability to detect polymorphism by Tanaka et al (2007), were used in this study ( Table 2). The PCR mixture was the same as for PS-ID analysis, but the concentration of primers was 0.5 μM.…”

Section: Rapd and Ssr Analysismentioning

confidence: 99%

“…In the last decade, the genetic diversity and phylogenetic relationship between various groups of cultivated melon have been studied using analysis of molecular polymorphism, which can be easily detected using various types of DNA markers, such as random amplified polymorphic DNA (RAPD: Dhillon et al, 2007;López-Sesé et al, 2003;Luan et al, 2008;Nakata et al, 2005;Soltani et al, 2010;Staub et al, 2000Staub et al, , 2004Stepansky et al, 1999;Tanaka et al, 2007;Yi et al, 2009), simple sequence repeats (SSRs: Dhillon et al, 2007;Garcia-Mas et al, 2004;López-Sesé et al, 2002;Monforte et al, 2003;Nakata et al, 2005;Staub et al, 2000), and amplified fragment length polymorphism (AFLP: Garcia-Mas et al, 2000;Yashiro et al, 2005). These results showed distinct genetic differentiation between subsp.…”

Section: Introductionmentioning

confidence: 99%

Molecular Analysis of the Genetic Diversity of Chinese Hami Melon and Its Relationship to the Melon Germplasm from Central and South Asia

Aierken

Akashi

Nhi

et al. 2011

J. Japan. Soc. Hort. Sci.

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Chinese Hami melon consists of the varieties cassaba, chandalak, ameri, and zard. To show their genetic diversity, 120 melon accessions, including 24 accessions of Hami melon, were analyzed using molecular markers of nuclear and cytoplasmic genomes. All Hami melon accessions were classified as the large-seed type with seed length longer than 9 mm, like US and Spanish Inodorus melon. Conomon accessions grown in east China were all the smallseed type. Both large-and small-seed types were in landraces from Iran, Afghanistan, Pakistan, and Central Asia. Analysis of an SNP in the PS-ID region (Rpl16-Rpl14) and size polymorphism of ccSSR7 showed that the melon accessions consisted of three chloroplast genome types, that is, maternal lineages. Hami melon accessions were T/338 bp type, which differed from Spanish melon and US Honey Dew (T/333 bp type), indicating a different maternal lineage within group Inodorus. The gene diversity (D), calculated from random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) polymorphism, was 0.476 in 120 melon accessions; the largest diversity was in Central Asian accessions (D = 0.377) but was low for Hami melon accessions (D = 0.243), even though Hami melon has diverse morphological traits, earliness, and shelf life. Reflecting such small genetic diversity, Hami melon accessions of vars. ameri and zard were grouped into cluster II, except for one accession, by the unweighted pair group method and the arithmetic mean (UPGMA) cluster analysis. Variety chandalak with distinct characters, such as early maturing and poor shelf life, was assigned to clusters IV and VI, indicating inter-varietal genetic differentiation within Hami melon. Three accessions from Turkmenistan and Afghanistan, with large seeds and T/338 bp type of chloroplast genome, were classified as cluster II with Hami melon accessions of vars. ameri and zard. We therefore concluded that Hami melon may have been transmitted from the west. The small-seed type melon of group Conomon grown in east China may have been introduced into China independently of Hami melon, because it had the A/338 bp type of the chloroplast genome and was clustered distantly from Hami melon according to nuclear genome analysis.

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“…C. melo is, therefore, considered the most diverse species in Cucumis (Stepansky et al 1999). Genetic diversity studies have used isozymes (Staub et al 1997;Akashi et al 2002), RFLPs (Zheng et al 1999), RAPDs (Garcia et al 1998;Stepansky et al 1999;Mliki et al 2001;López-Sesé et al 2003;Staub et al 2004;Sensoy et al 2007;Tanaka et al 2007), AFLPs (GarciaMas et al 2000), ISSR and simple-sequence repeat (SSR) (Katzir et al 1996;Staub et al 2000;Daning-Poleg et al 2001;López-Sesé et al 2002;Monforte et al 2003;Nakata et al 2005;Tzitzikas et al 2009) to analyze variability and relationships among melon groups. These studies have assisted in the elucidation of intraspecific relationships in melons from different origins.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity among melon accessions from Iran and their relationships with melon germplasm of diverse origins using microsatellite markers

et al. 2013

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Melon is one of the most important horticultural crops in Iran. There are a few studies on the genetic structure of Iranian melon. A set of 18 simple sequence repeat (SSR) primer pairs were used to assess the genetic diversity in a collection of 24 melon accessions representing different botanical groups of Iranian cultivated melons (vars. inodorus, cantalupensis and dudaim), along with 28 reference accessions from diverse geographic origin. All studied SSR loci were polymorphic that confirmed their usefulness for genetic analysis of melons. A total number of 141 alleles were detected, with an average of 7.8 alleles per locus for reference genotypes and 4.38 alleles per locus for Iranian accessions. The low variability within Iranian melon accessions is reflected by the low values of the observed heterozygosity (with an average of 0.119), indicating lack of intercrossing between accessions or a high rate of self-pollination. Values of observed homozygosity for ''Suski-e-Sabz'' and ''Khatouni'', as the most cultivated melon in Iran, were 0.98 and 0.99, respectively. Cluster analysis divided Iranian accessions into two major groups. The highest level of polymorphism was detected among the dudaim group. The analysis of molecular variance indicated that the majority of variation (87 %) was due to the difference within accessions. The average pairwise genetic distance among Iranian accessions was 0.674.Our results showed a distinct separation of dudaim group from the rest of Iranian accessions, even separated two different groups of var. dudaim with different traits. There was a wide genetic distance between Honey Dew, as the most popular member of inodorus group worldwide and ''Khatouni'', a major Iranian winter melon (GD = 0.809). This genetic distance shows the importance of Iranian accessions for conservation and use in breeding programs.

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Molecular characterization of South and East Asian melon, Cucumis melo L., and the origin of Group Conomon var. makuwa and var. conomon revealed by RAPD analysis

Cited by 55 publications

References 28 publications

Botanical Identification and Genetic Diversity in Melons From Family Farming in the State of Maranhão

Botanical Identification and Genetic Diversity in Melons From Family Farming in the State of Maranhão

Molecular Analysis of the Genetic Diversity of Chinese Hami Melon and Its Relationship to the Melon Germplasm from Central and South Asia

Genetic diversity among melon accessions from Iran and their relationships with melon germplasm of diverse origins using microsatellite markers

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