Molecular characterization of Staphylococcus aureus isolated from meat, milk and their products

Abstract

This study was planned to throw the light on the incidence of S. aureus in 485 random samples of meat, milk and their products, The bacteriological examination of 200 meat and meat products revealed that the prevalence of S. aureus was 19 isolates (9.5%), The bacteriological examination of 285 milk and milk product revealed that the prevalence of S. aureus was16 isolates (18.8%). The prevalence of S. aureus in examined milk collected from (farms, dairy shops and street vendors) were (16%, 16% and 22.8%) respectively, While milk products showed that, the prevalence of S. aureus was 8 isolates (4%). All isolated strains were identified as S. aureus by using specific culture media and biochemical tests. Detection of enterotoxin in S. aureus isolates proved that, 27out of 43 of them were entrotoxignic , 15 isolates out of 27 were enterotoxin A producer,7 isolates were enterotoxin C producer and 5 isolates were harbored the type (A and C). PCR used for confirmation of S. aureus isolates, revealed that S. aureus had four DNA fragments patterns of 410, 740, 910 and 970 b.p. with primer (1) meanwhile with primer (2), the pattern of S. aureus had two bands of 562 and 928 b.p. Studying the antibiotic sensitivity test of S. aureus for 15 different types of antibiotics in lab, the result revealed that most strains which isolated from meat, milk and their products were show highly degree of resistant to Methicillin followed by Oxacillin, Chloramphenicol, Amoxiciilin, Cephranin, Cephalothin, Amikacin, Gentamycin, Ciprofloxacin, doxycycline while they were sensitive to Cefaclor, Streptomycin, Norfloxacin, Erythromycin and trimethoprim-sulphamethoxazole. PCR also, confirmed the presence of mec A gene in 4 isolates (methicillin resistant S. aureus).

Phylogenetic Tracking of Ica-locus in MRSA from Different Sources at Ismailia City, Egypt

“…The occurrence of Staphylococcus in raw milk and raw meat may be linked to filthy worker hands, poor water quality used to wash utensils and carcasses, unsanitary transportation, improper storage, and contamination from mastitic cattle resuming to the milking practices. The current findings are consistent with the findings of (Normanno et al, 2007;De Boer et al, 2009;Ezzat et al, 2014). In this respect our results were less than the results of (Fa Mansour and Am Basha, 2009) and (Bhargava et al, 2011) who discovered that S. aureus was present in 20% and 20.5% of meat samples from different markets, respectively.…”

“…On the other hand, our results were nearly to (Goja et al, 2013) and (Ezzat et al, 2014) who retrieved S. aureus from raw beef meal in percentages of 5.55%, 12%, and 10%, respectively, and were less than the outcomes of (Kitai et al, 2005;Van Loo et al, 2007;Pu et al, 2009) that was 65%, 46% , 39.2% respectively. Therefore in this paper, the frequency of Staphylococcus recovered from unpasteurized milk is lower than the frequency of Staphylococcus recovered from pasteurized milk.…”

“…(Fagundes et al, 2010) who confirmed that 7.3% of those likely Staphylococcus could transfer to consumers through milk from animals with mild mastitis. This result is noticeably lower than the findings of (Singh and Baxi, 1982) (Ezzat et al, 2014) They reported that the incidence of Staphylococcus in raw milk samples was (21%) and (18.5%). The findings of this study differ from those of previous studies undertaken by (Abou-Khadra et al, 2020) discovered that S. aureus was present in 20% of raw milk samples from Sharkia Governorate.…”

“…The prevalence of MRSA in this research was greater than in previous studies by (Ezzat et al, 2014) who discovered 96 patients with MRSA from 348 patients with S. aureus bacteremia, (El-Jakee et al, 2008) who demonstrated that S. aureus isolates from patients were sensitive to methicillin (Lee et al, 2001) who said that MRSA is a major problem in Korea, the rate of methicillin resistance among human S. aureus isolates in Korea is greater than 50%. In addition to, (Normanno et al, 2007;Van Loo et al, 2007;De Boer et al, 2009;Pu et al, 2009) they observed that MRSA was present in 3.75%, 2%, 11.9%, and 5% of raw beef samples, respectively.…”

Studies on bacteriological profile of chicken meat cuts in Kaliobia governorate

“…Several workers have reported the occurrence of multidrug resistant S.aureus in poultry (Waters et al, 2011). As shown in table (5) the antimicrobial sensitivity for isolated S.aureus strains were agreed with Akbar and Anal (2013); Abd El-Salam-Azza (2014); Ezzat et al (2014); Abdalrahman et al 2015and Afifi-Dina (2016). In addition, the results proved that multiple antibiotic resistances are widely spread among isolated strains of S.aureus.…”