Molecular Characterization of Streptococcus agalactiae Isolates From Pregnant and Non-Pregnant Women at Yazd University Hospital, Iran

Abstract

Background:Streptococcus agalactiae (Group B streptococcus, GBS) that colonize the vaginas of pregnant women may occasionally cause neonatal infections. It is one of the most common causes of sepsis and meningitis in neonates and of invasive diseases in pregnant women. It can also cause infectious disease among immunocompromised individuals. The distribution of capsular serotypes and genotypes varies over time and by geographic era. The serotyping and genotyping data of GBS in Iranian pregnant and non-pregnant women seems very limited.Objectives:The aim of this study was to investigate the GBS ‎molecular capsular serotype ‎and genotype distribution of pregnant and non-pregnant carrier ‎women at Yazd university hospital, in Iran.‎Patients and Methods:In this cross-sectional study, a total of 100 GBS strains isolated from 237 pregnant and 413 non-pregnant women were investigated for molecular capsular serotypes and surface protein genes using the multiplex PCR assay. The Chi-square method was used for statistical analysis.Results:Out of 650 samples, 100 (15.4%) were identified as GBS, with a predominance of capsular serotypes III (50%) [III-1 (49), III-3 (1)], followed by II (25%), Ia (12%), V (11%), and Ib (2%), which was similar with another study conducted in Tehran, Iran, but they had no serotype Ia in their report. The surface protein antigen genes distribution was rib (53%), epsilon (38%), alp2/3 (6%), and alpha-c (3%).Conclusions:The determination of serotype and surface proteins of GBS strains distribution would ‎be ‎relevant ‎for the future possible formulation of a GBS vaccine.

Determination of surface proteins profile, capsular genotyping, and antibiotic susceptibility patterns of Group B Streptococcus isolated from urinary tract infection of Iranian patients

“…Approximately most of GBS strains possess one of alp genes. Our multiplex PCR analysis results showed that the frequency of rib in GBS isolates was 40%, as predominant type that was similar to other geographical regions of Iran, Chinaand Italy [3, 37, 38]. Only 1% of the tested isolates were negative for any existing alp genes and 4% were positive for two genes.…”

Molecular characterization of Streptococcus agalactiae isolated from pregnant women and newborns at the University of Gondar Comprehensive Specialized Hospital, Northwest Ethiopia

“…However, it is different from a study conducted before three decades in the same hospital where 60% were type Ib followed by type Ia (16%) [37]; in Poland, serotype III (35%) was the most prevalent types followed by Ia (20%), V (17%), II (15%), Ib (8%) and IV (5%) [38]. Similarly, a study from Iran showed that serotype III (50%) was the most common serotype, followed by serotype II (25%), Ia (12%), V (11%), and Ib (2%) [39].…”

Clinical characteristics and molecular epidemiology of invasive Streptococcus agalactiae infections between 2007 and 2016 in Nara, Japan

“…The ST and clonal complex (CC) of each isolate were determined using the GBS MLST database (http://pubmlst.org/sagalactiae/) and eBURST V3 (http://eburst.mlst.net/v3/enter_data/single/). VIII AGGTCAACCACTATATAGCGA TCTTCAAATTCCGCTGACTT cps8J [16] dltS AGGAATACCAGGCGATGAACCGAT TGCTCTAATTCTCCCCTTATGGC dltS [17] adhP GGTGTGTGCCATACTGATTT ACAGCAGTCACAACCACTCC adhP [17] pheS ATATCAACTCAAGAAAAGCT TGATGGAATTGATGGCTATG pheS [17] Atr ATGGTTGAGCCAATTATTTC CCTTGCTCAACAATAATGCC atr [17] glnA AATAAAGCAATGTTTGATGG GCATTGTTCCCTTCATTATC glnA [17] sdhA AACATAGCAGAGCTCATGAT GGGACTTCAACTAAACCTGC sdhA [17] glcK GGTATCTTGACGCTTGAGGG ATCGCTGCTTTAATGGCAGA glcK [17] Tkt ACACTTCATGGTGATGGTTG TGACCTAGGTCATGAGCTTT tkt [17] ermA AACTTGTGGAAATGAGTCAACGG CAGAATCTACATTAGGCTTAGGG ermA [19] ermB ATTGGAACAGGTAAAGGGCG GAACATCTGTGGTATGGCG ermB [19] mefA AGTATCATTAATCACTAGTGC TTCTTCTGGTACTAAAAGTGG mefA [19] gyrA GGTTTAAAACCTGTTCATCGTCGT GCAATACCAGTTGCACCATTGACT gyrA [20] parC CCGGATATTCGTGATGGCTT TGACTAAAAGATTGGGAAAGGC parC [20] https://doi.org/10.1371/journal.pone.0240590.t001…”