Molecular characterization of ten zinc (Zn) transporter genes and their regulation to Zn metabolism in freshwater teleost yellow catfish Pelteobagrus fulvidraco

Chen, Shu-Wei; Wu, Kun-Lung; Lv, Wu-Hong; Song, Chang-Chun; Luo, Zhi

doi:10.1016/j.jtemb.2019.126433

Cited by 19 publications

(17 citation statements)

References 53 publications

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“…Our study indicated that high dietary nano-ZnO addition increased intestinal Zn content, in agreement with other studies using nano-Zn [ 12 ] and an inorganic Zn source [ 24 ]. To explore the mechanism of Zn-induced changes in Zn content, several genes and proteins related to endocytosis were investigated.…”

Section: Discussionsupporting

confidence: 92%

“…Second, we found that high dietary nano-ZnO addition tended to reduce mRNA levels of zip1 , zip4, and zip14 . The ZIP family plays prominent roles in Zn influx into cytosols [ 9 , 24 ]. Thus, the reduction in their mRNA levels will downregulate excessive Zn transport into the cytosols and help to maintain Zn homeostasis, as suggested by Chen et al [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

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Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

Chen

et al. 2021

IJMS

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Nano-sized zinc oxide (nano-ZnO) affects lipid deposition, but its absorption patterns and mechanisms affecting lipid metabolism are still unclear. This study was undertaken to investigate the molecular mechanism of nano-ZnO absorption and its effects on lipid metabolism in the intestinal tissues of a widely distributed freshwater teleost yellow catfish Pelteobagrus fulvidraco. We found that 100 mg/kg dietary nano-ZnO (H-Zn group) significantly increased intestinal Zn contents. The zip6 and zip10 mRNA expression levels were higher in the H-Zn group than those in the control (0 mg/kg nano-ZnO), and zip4 mRNA abundances were higher in the control than those in the L-Zn (50 mg/kg nano-ZnO) and H-Zn groups. Eps15, dynamin1, dynamin2, caveolin1, and caveolin2 mRNA expression levels tended to reduce with dietary nano-ZnO addition. Dietary nano-ZnO increased triglyceride (TG) content and the activities of the lipogenic enzymes glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), and isocitrate dehydrogenase (ICDH), upregulated the mRNA abundances of lipogenic genes 6pgd, fatty acid synthase (fas), and sterol regulatory element binding protein 1 (srebp1), and reduced the mRNA expression of farnesoid X receptor (fxr) and small heterodimer partner (shp). The SHP protein level in the H-Zn group was lower than that in the control and the L-Zn group markedly. Our in vitro study indicated that the intestinal epithelial cells (IECs) absorbed nano-ZnO via endocytosis, and nano-Zn-induced TG deposition and lipogenesis were partially attributable to the endocytosis of nano-ZnO in IECs. Mechanistically, nano-ZnO-induced TG deposition was closely related to the metal responsive transcription factor 1 (MTF-1)-SHP pathway. Thus, for the first time, we found that the lipogenesis effects of nano-ZnO probably depended on the key gene shp, which is potentially regulated by MTF1 and/or FXR. This novel signaling pathway of MTF-1 through SHP may be relevant to explain the toxic effects and lipotoxicity ascribed to dietary nano-ZnO addition.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

Chen

et al. 2021

IJMS

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“…Similarly, several studies reported that ZIP3 transcription and mRNA expression increased in responses to Zn deficiency [ 14 , 15 ], but reduced as Zn concentration increased [ 16 ]. In our laboratory, Chen et al [ 5 ] found Zn addition down-regulated mRNA levels of ZIP3 compared to the control in the hepatocytes. These results are consistent with the reported functions of these proteins in maintaining the homeostasis of Zn.…”

Section: Discussionmentioning

confidence: 89%

“…According to the full-length ZIP3 and ZIP8 cDNA sequences obtained in the studies [ 5 ], RNA ligase-mediated rapid amplification of 5′ cDNA ends (RLM-5′RACE) method was used to identify their 5′ cDNA sequences and the transcription start sites (TSS). The promoter cloning followed the method described in Xu et al [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

“…Zn uptake is mediated by Zn transporters of the ZIP (zinc-regulated transporters, iron-regulated transporter-like protein) family, which increase the cytoplasmic Zn concentration by transporting Zn from the extracellular space or organelles into cytoplasma [ 3 , 4 ]. Among these members, ZIP3 and ZIP8 were widely expressed in many tissues and involved in Zn acquisition by many cells of the body [ 4 , 5 ]. However, ZIP3 and ZIP8 possess many differences in the tissue distribution, expression, subcellular localization and regulation.…”

Section: Introductionmentioning

confidence: 99%

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Functional Analysis of Two Zinc (Zn) Transporters (ZIP3 and ZIP8) Promoters and Their Distinct Response to MTF1 and RREB1 in the Regulation of Zn Metabolism

Chen

et al. 2020

IJMS

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ZIP (zinc-regulated transporters, iron-regulated transporter-like protein) family plays an important role in organism Zn balance. This research identified the promoter regions of ZIP3 and ZIP8, two members of ZIP family, from a freshwater teleost yellow catfish Pelteobagrus fulvidraco, characterized the binding sequences of the metal-responsive transcription factor-1 (MTF-1) and Ras responsive element binding protein 1 (RREB1) on their promoter regions. The present study cloned and obtained the 2027 bp of ZIP3 promoter and 1664 bp of ZIP8 promoter, and predicted several key elements on their promoters, such as the binding sites of CREB (cAMP-response element binding protein), KLF4 (Kruppel like factor 4), MTF-1 and RREB1. The sequence deletion from −361 bp to −895 bp down-regulated the luciferase activity of ZIP3 promoter, and the deletion from −897 bp to −1664 bp down-regulated the luciferase activity of ZIP8 promoter. Within different deletion plasmids, the relative luciferase activities of ZIP3 and ZIP8 promoters changes to Zn incubation in a Zn concentration-dependent manner. The site mutagenesis and EMSA (electrophoretic mobility shift assay) found that the −1327 bp/−1343 bp MTF-1 binding site and the −248 bp/−267 bp RREB1 binding site on the ZIP3 promoter, and the −1543 bp/−1557 bp MTF-1 binding site on the ZIP8 promoter are functional sites. Low Zn increased the binding capability between MTF-1 and its responsive site on the ZIP3 promoter, and high Zn increased the transcriptional activation ZIP3 by RREB1; Zn also promoted the binding ability between MTF-1 and its responsive element on the ZIP8 promoter. This study provides the first direct evidence for the response elements of MTF-1 and RREB1 on ZIP3 and MTF-1 on ZIP8 to Zn, which are very important for the evaluation of Zn nutrition and toxicity in vertebrates.

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A Zinc Uptake Transporter ZIP1-II Is Involved in Zinc Accumulation in the Hepatopancreas of Pacific Oyster Crassostrea gigas

Kong,

Luo,

Wang

et al. 2024

Mar Biotechnol

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Molecular characterization of ten zinc (Zn) transporter genes and their regulation to Zn metabolism in freshwater teleost yellow catfish Pelteobagrus fulvidraco

Cited by 19 publications

References 53 publications

Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

Functional Analysis of Two Zinc (Zn) Transporters (ZIP3 and ZIP8) Promoters and Their Distinct Response to MTF1 and RREB1 in the Regulation of Zn Metabolism

A Zinc Uptake Transporter ZIP1-II Is Involved in Zinc Accumulation in the Hepatopancreas of Pacific Oyster Crassostrea gigas

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