Molecular characterization of the capsular antigens of Pasteurella multocida isolates using multiplex PCR

Almaary, Khalid S.; Dawoud, Turki M.; Mubarak, Ayman; Hessain, Ashgan M.; Galal, Hussein M.; Kabli, Saleh A.; Mohamed, Moussa I.

doi:10.1016/j.sjbs.2016.06.006

Cited by 10 publications

(9 citation statements)

References 8 publications

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“…However, P. multocida was found only in nine out of 67 samples (13%). PCR group and PCR for capsular type detection was found to be a rapid and sensitive method, as reported earlier by Townsend et al [13] and Al-Maary et al [19].…”

Section: Discussionsupporting

confidence: 69%

Isolation and Identification of Mannheimia haemolytica and Pasteurella multocida Species from Ruminants in Six Different Regions in Morocco

Sebbar¹,

Zro²,

Kichou³

et al. 2018

JAST-A

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Pasteurella species is considered the principal pathogen of the respiratory tract. Mannheimia haemolytica and Pasteurella multocida were investigated and typed from nasal swabs and tissues taken from sheep, goat and cattle. Indeed, 41 lung and 121 nasal swabs samples were collected from animals with respiratory diseases during 2015 to 2017 in six different regions in Morocco. At first, a screening of Pasteurella species using the real time PCR (RT-PCR) was carried out, then all isolated strains on agar blood were confirmed by PCR gel based assay specific for M. haemolytica and P. multocida. Pathogenicity was evaluated in mice and histopathological examination was done on some of lung tissue. The results revealed that 34 samples of which 28 (55%) from nasal swabs and six (38%) from lungs were positive for M. haemolytica and nine samples of which seven (14%) from nasal swabs and two (13%) from lungs were positive on P. multocida serogroup A. Seventy-two percent (72%) isolates were highly pathogenic to mice, which is in accordance with the results obtained by histopathology examination. This is the first report for widespread infections of Pasteurella (M. haemolytica & P. multocida) in ruminants in Morocco. Therefore, measures including development of vaccines are highly required to mitigate the impact of the bacteria in animals.

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Section: Discussionsupporting

confidence: 69%

Isolation and Identification of Mannheimia haemolytica and Pasteurella multocida Species from Ruminants in Six Different Regions in Morocco

Sebbar¹,

Zro²,

Kichou³

et al. 2018

JAST-A

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“…is typing system involves the hyaD-hyaC gene (involved with hyaluronic acid synthesis) for capsular serogroup A, the dcbF gene (involved in heparin synthesis) for capsular serogroup D, the fcbD gene (involved in chondroitin production) for capsular serogroup F, and the bcbD and ecbJ genes (both involved in glycosyltransferase synthesis) for capsular serogroups B and E, respectively [9]. e diagnosis of HS poses challenges due to the range of clinical appearances and complex laboratory protocols [10].…”

Section: Introductionmentioning

confidence: 99%

“…The diagnosis of HS poses challenges due to the range of clinical appearances and complex laboratory protocols [ 10 ]. The detection and characterization of P. multocida have depended on the use of traditional biochemical and serological procedures as well as, in recent times, molecular assays [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection and Characterization of Pasteurella multocida Infecting Camels in Marsabit and Turkana Counties, Kenya

Kasivalu

Omwenga

Aboge

2022

International Journal of Microbiology

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Pasteurella multocida infection is common in Kenya though there is little knowledge of the genetic diversity of the pathogen. P. multocida is part of the normal flora in the respiratory tract of camels, but it becomes pathogenic when the resistance of the camel body is diminished by bad ecological conditions. This study was conducted to detect, characterize, and determine the genetic diversity of P. multocida infecting camels in Marsabit and Turkana Counties. The KMT1 gene was targeted as the marker gene for P. multocida and hyaD-hyaC, bcbD, dcbF, ecbJ, and fcbD as marker genes for capsular serogroups A, B, D, E, and F, respectively. Out of 102 blood and 30 nasal swab samples, twenty-one samples (16%) were confirmed to be positive for P. multocida and only capsular group E was detected in both counties. The P. multocida sequences were highly conserved and were related to strains from other parts of the world. Our study has confirmed that camels in Marsabit and Turkana Counties of Kenya are infected by P. multocida of capsular type E. Farmers should not underfeed camels, ensure appropriate medication and vaccination programs, and minimize herding of camels in crowded areas especially in wet conditions in order to slow the spread of P. multocida infection.

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“…Postmortem examination has elucidated extreme congestion of the trachea, lungs, small intestine, and liver [ 5 ]. The diagnosis of pasteurellosis in cattle is based on clinical manifestations, postmortem examinations, and traditional bacteriological techniques, which are considered time-consuming and lacking in reliability [ 6 ]. The typing of P. multocida is based on the capsule and cell envelope lipopolysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Virulence Determinants and Antimicrobial Profiles of Pasteurella multocida Isolated from Cattle and Humans in Egypt

et al. 2021

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Pasteurella multocida is a Gram-negative bacterium that causes drastic infections in cattle and humans. In this study, 55 isolates were recovered from 115 nasal swabs from apparently healthy and diseased cattle and humans in Minufiya and Qalyubia, Egypt. These isolates were confirmed by kmt1 existence, and molecular classification of the capsular types showed that types B, D, and E represented 23/55 (41.8%), 21/55 (38.1%), and 11/55 (20.0%), respectively. The isolates were screened for five virulence genes with hgbA, hgbB, and ptfA detected in 28/55 (50.9%), 30/55 (54.5%), and 25/55 (45.5%), respectively. We detected 17 capsular and virulence gene combinations with a discriminatory power (DI) of 0.9286; the most prevalent profiles were dcbF type D and dcbF type D, hgbA, hgbB, and ptfA, which represented 8/55 (14.5%) each. These strains exhibited high ranges of multiple antimicrobial resistance indices; the lowest resistances were against chloramphenicol, ciprofloxacin, amoxicillin/clavulanic acid, and levofloxacin. The macrolide–lincosamide–streptogramin B methylase gene erm(Q), with erm(42) encoding MLSB monomethyltransferase, mph(E) encoding a macrolide efflux pump, and msr(E) encoding macrolide-inactivating phosphotransferase were present. The class 1 and 2 integrons and extended-spectrum β-lactamase genes intl1, intl2, blaCTX-M, blaCTX-M-1, and blaTEM were detected. It is obvious to state that co-occurrence of resistance genes resulted in multiple drug-resistant phenotypes. The identified isolates were virulent, genetically diverse, and resistant to antimicrobials, highlighting the potential risk to livestock and humans.

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Molecular characterization of the capsular antigens of Pasteurella multocida isolates using multiplex PCR

Cited by 10 publications

References 8 publications

Isolation and Identification of Mannheimia haemolytica and Pasteurella multocida Species from Ruminants in Six Different Regions in Morocco

Isolation and Identification of Mannheimia haemolytica and Pasteurella multocida Species from Ruminants in Six Different Regions in Morocco

Molecular Detection and Characterization of Pasteurella multocida Infecting Camels in Marsabit and Turkana Counties, Kenya

Virulence Determinants and Antimicrobial Profiles of Pasteurella multocida Isolated from Cattle and Humans in Egypt

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