Molecular characterization of the complete genome of a novel partitivirus hosted by the saprobic mushroom Leucocybe candicans

Şahin, Ergin; Keskin, Emre; Akata, İlgaz

doi:10.1007/s00203-021-02540-y

Cited by 7 publications

(3 citation statements)

References 22 publications

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“…Partitiviruses are known to be characterized by conserved 5 regions on the plus strands of RNA1 and RNA2 [108,109]. Alphapartitiviruses, to which beet cryptic virus 1 belongs, have a genus-specific sequence GAWNW at the 5 end [110].…”

Section: Detection Of Novel Grapevine Viruses Grapevine Alphapartitiv...mentioning

confidence: 99%

Grapevine Virome of the Don Ampelographic Collection in Russia Has Concealed Five Novel Viruses

Belkina,

Karpova,

Porotikova

et al. 2023

Viruses

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In this study, an analysis of the virome of 51 grapevines from the Don ampelographic collection named after Ya. I. Potapenko (Russia) was performed using high-throughput sequencing of total RNA. A total of 20 previously described grapevine viruses and 4 viroids were identified. The most detected were grapevine rupestris stem pitting-associated virus (98%), hop stunt viroid (98%), grapevine Pinot gris virus (96%), grapevine yellow speckle viroid 1 (94%), and grapevine fleck virus (GFkV, 80%). Among the economically significant viruses, the most present were grapevine leafroll-associated virus 3 (37%), grapevine virus A (24%), and grapevine leafroll-associated virus 1 (16%). For the first time in Russia, a grapevine-associated tymo-like virus (78%) was detected. After a bioinformatics analysis, 123 complete or nearly complete viral genomes and 64 complete viroid genomes were assembled. An analysis of the phylogenetic relationships with reported global isolates was performed. We discovered and characterized the genomes of five novel grapevine viruses: bipartite dsRNA grapevine alphapartitivirus (genus Alphapartitivirus, family Partitiviridae), bipartite (+) ssRNA grapevine secovirus (genus Fabavirus, family Secoviridae) and three (+) ssRNA grapevine umbra-like viruses 2, -3, -4 (which phylogenetically occupy an intermediate position between representatives of the genus Umbravirus and umbravirus-like associated RNAs).

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Section: Detection Of Novel Grapevine Viruses Grapevine Alphapartitiv...mentioning

confidence: 99%

Grapevine Virome of the Don Ampelographic Collection in Russia Has Concealed Five Novel Viruses

Belkina,

Karpova,

Porotikova

et al. 2023

Viruses

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“…Mutualism is also common between mycoviruses and their hosts [ 3 , 4 ]. Many edible mushroom species are infected with mycoviruses, including Agaricus bisporus , Lentinula edodes , Pleurotus ostreatus , Flammulina velutipes , Pleurotus eryngii , Agrocybe aegerita , Boletus edulis , Volvariella volvacea , Grifola frondose , Armillaria species., Auricularia heimuer , Bondarzewia berkeleyi , Picoa juniperi , Leucocybe candicans , Cordyceps chanhua , and Pleurotus citrinopileatus [ 1 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. These mycoviruses can enhance or diminish various symptoms in the host fungi, including changes in morphology, sporulation, pigmentation, radial growth, and/or biomass production [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Oyster Mushroom Spherical Virus Crosses the Species Barrier and Is Pathogenic to a New Host Pleurotus pulmonarius

Zhang,

Hu,

Zhao

et al. 2023

IJMS

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Oyster mushroom spherical virus (OMSV) is a mycovirus with a positive-sense single-stranded RNA genome that infects the edible mushroom Pleurotus ostreatus. OMSV is horizontally transferred from an infected strain to a cured strain via mycelia. The infection results in significant inhibition of mycelial growth, malformation of fruiting bodies, and yield loss in oyster mushrooms. This study successfully transferred OMSV from P. ostreatus to Pleurotus pulmonarius. However, transmission was not successful in other Pleurotus species including P. citrinopileatus, P. eryngii, P. nebrodensis, and P. salmoneostramineus. The successful OMSV infection in P. pulmonarius was further verified with Western blot analysis using a newly prepared polyclonal antiserum against the OMSV coat protein. Furthermore, OMSV infection reduced the mycelial growth rate of P. pulmonarius. The OMSV-infected strain demonstrated abnormal performance including twisted mushrooms or irregular edge of the cap as well as reduced yield of fruiting bodies in P. pulmonarius, compared to the OMSV-free strain. This study is the first report on the infection and pathogenicity of OMSV to the new host P. pulmonarius. The data from this study therefore suggest that OMSV is a potential threat to P. pulmonarius.

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“…The mycovirus was first found and isolated in the diseased mushrooms of Agaricus bisporus [ 7 ]. With the increasing number of edible mushroom species, several others have been reported to be infected with mycoviruses including Pleurotus ostreatus , Lentinula edodes , Flammulina velutipes , Cyclocybe aegerita , Volvariella volvacea , Boletus edulis , Armillaria , Grifola frondose , Auricularia heimuer , Leucocybe candicans, Picoa juniperi, and Bondarzewia berkeleyi [ 3 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. The majority of known mycoviruses are latent and their infections show no apparent symptoms in their hosts.…”

Section: Introductionmentioning

confidence: 99%

Multiplex Detection of Pleurotus ostreatus Mycoviruses

Zhang

Wang

et al. 2022

CIMB

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Mycoviruses are viruses that specifically infect and replicate in fungi. Several mycoviruses have been previously reported in Pleurotus ostreatus, including the oyster mushroom spherical virus (OMSV), oyster mushroom isometric virus (OMIV), Pleurotus ostreatus spherical virus (POSV), and Pleurotus ostreatus virus 1 (PoV1). This study was designed to develop a multiplex RT-PCR for simultaneous detection and differentiation of the four P. ostreatus mycoviruses. Four pairs of primers were designed from conserved regions based on the reported sequences and the multiplex RT-PCR products were 672 bp for OMSV, 540 bp for OMIV, 310 bp for POSV, and 200 bp for PoV1. The optimal annealing temperature of the multiplex RT-PCR was 62 °C and the detection limits of the plasmids were 100 fg for OMSV and OMIV and 1 pg for POSV and PoV1. This technique was successfully applied for the detection of OMSV, OMIV, and POSV from different P. ostreatus strains and the plasmid containing the PoV1 sequence. This methodology can serve as a powerful diagnostic tool for the survey of the incidence and epidemiology of the four P. ostreatus mycoviruses, further contributing to the prevention and treatment of mycoviral diseases in P. ostreatus.

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Molecular characterization of the complete genome of a novel partitivirus hosted by the saprobic mushroom Leucocybe candicans

Cited by 7 publications

References 22 publications

Grapevine Virome of the Don Ampelographic Collection in Russia Has Concealed Five Novel Viruses

Grapevine Virome of the Don Ampelographic Collection in Russia Has Concealed Five Novel Viruses

Oyster Mushroom Spherical Virus Crosses the Species Barrier and Is Pathogenic to a New Host Pleurotus pulmonarius

Multiplex Detection of Pleurotus ostreatus Mycoviruses

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