Molecular characterization of the first satellite DNA with CENP‐B and CDEIII motifs in the bat <i>Pipistrellus kuhli</i>

Fantaccione, Stefania; Pontecorvo, Giovanni; Zampella, Vincenzo

doi:10.1016/j.febslet.2005.03.064

Cited by 6 publications

(3 citation statements)

References 47 publications

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“…For example, seven divergent horse satDNAs exibit CENP B box variants with identity in 9–12 out of 17 nucleotide of human CENP B box [39]. Presence of motifs similar to the CENP-B box has also been detected in a number of satDNAs from diverse species outside mammals [40], [41]. In examined nematode species, homology of Box 1 with the human CENP-B box is in the same range found for the CENP-B box in diverse mammalian species [39].…”

Section: Discussionmentioning

confidence: 99%

Conserved DNA Motifs, Including the CENP-B Box-like, Are Possible Promoters of Satellite DNA Array Rearrangements in Nematodes

et al. 2013

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Tandemly arrayed non-coding sequences or satellite DNAs (satDNAs) are rapidly evolving segments of eukaryotic genomes, including the centromere, and may raise a genetic barrier that leads to speciation. However, determinants and mechanisms of satDNA sequence dynamics are only partially understood. Sequence analyses of a library of five satDNAs common to the root-knot nematodes Meloidogyne chitwoodi and M. fallax together with a satDNA, which is specific for M. chitwoodi only revealed low sequence identity (32–64%) among them. However, despite sequence differences, two conserved motifs were recovered. One of them turned out to be highly similar to the CENP-B box of human alpha satDNA, identical in 10–12 out of 17 nucleotides. In addition, organization of nematode satDNAs was comparable to that found in alpha satDNA of human and primates, characterized by monomers concurrently arranged in simple and higher-order repeat (HOR) arrays. In contrast to alpha satDNA, phylogenetic clustering of nematode satDNA monomers extracted either from simple or from HOR array indicated frequent shuffling between these two organizational forms. Comparison of homogeneous simple arrays and complex HORs composed of different satDNAs, enabled, for the first time, the identification of conserved motifs as obligatory components of monomer junctions. This observation highlights the role of short motifs in rearrangements, even among highly divergent sequences. Two mechanisms are proposed to be involved in this process, i.e., putative transposition-related cut-and-paste insertions and/or illegitimate recombination. Possibility for involvement of the nematode CENP-B box-like sequence in the transposition-related mechanism and together with previously established similarity of the human CENP-B protein and pogo-like transposases implicate a novel role of the CENP-B box and related sequence motifs in addition to the known function in centromere protein binding.

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Section: Discussionmentioning

confidence: 99%

Conserved DNA Motifs, Including the CENP-B Box-like, Are Possible Promoters of Satellite DNA Array Rearrangements in Nematodes

et al. 2013

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“…Other satellite DNAs have been found to have regions homologous to both CDEIII and to be associated with a CENP-B box motif in Lacerta gracea (Capriglione et al, 1998), in the scallop Adamussium colbecki (Canapa et al, 2000), and in the bat Pipistrellus kuhli (Fantaccione et al, 2005). The presence of this CDEIII motif and the CENP-B box points to a role in determining the centromere function, but no data is available at present on the implications of their combined presence in this centromeric function or their evolutionary relationship (Fantaccione et al, 2005). However, the CDEIII motif sequences comparison found in the different analysed species (reptile, scallop and bat) did not reveal significant results showing low homology among them ( Fig.…”

Section: Discussionmentioning

confidence: 99%

The centromeric satellite of the wedge sole (<i>Dicologoglossa cuneata</i>, Pleuronectiformes) is composed mainly of a sequence motif conserved in other vertebrate centromeric DNAs

Herrán

Robles²,

Navas³

et al. 2008

Cytogenet Genome Res

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Here, a new satellite-DNA family is isolated and characterized from wedge sole, Dicologoglossa cuneata Moreau, 1881 (Pleuronectiformes), a fish having a small genome. This satellite-DNA family of sequences was isolated by conventional cloning after digestion of genomic DNA with the DraI restriction enzyme. Repeat units are 171 bp in length with a high AT content (63%). Several runs of consecutive adenines and thymines were found, and concomitantly computer analyses revealed that these regions are prone to acquire stable sequence-directed curvature. Especially remarkable is that the DraI sequences are composed almost entirely of the repetition of up to fourteen 9-bp motifs (T/C)GTC(A/C)AAAA similar to other vertebrate centromeric satellite-DNA sequences. In fact, we demonstrate the origin of this satellite through duplication of this motif plus the addition of a stretch of cytosines. The centromeric location and the presence in this satellite-DNA sequence of not only different vertebrate motifs (CENP-B box, pJα) but also others such as the CDEIII motif of Saccharomyces cerevisiae reveal a possible role in centromere function. All these characteristics provide important information on the origin, function, and the evolution of the centromeric satellite DNAs in wedge sole.

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“…The monomer unit is 1100 bp long and contains the CENP-B box as well as subrepeats, palindromes, and AT-rich tracts. The monomers group into two clusters (Fantaccione et al, 2005). Both pipistrelle satellites are absent in the genomes of other bat species analyzed, thus revealing that they might be species-specific.…”

Section: Chiropteramentioning

confidence: 99%

Centromere Evolution: Digging into Mammalian Primary Constriction

Giannuzzi¹,

Catacchio²,

Ventura³

2012

Current Frontiers and Perspectives in Cell Biology

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Molecular characterization of the first satellite DNA with CENP‐B and CDEIII motifs in the bat Pipistrellus kuhli

Cited by 6 publications

References 47 publications

Conserved DNA Motifs, Including the CENP-B Box-like, Are Possible Promoters of Satellite DNA Array Rearrangements in Nematodes

Conserved DNA Motifs, Including the CENP-B Box-like, Are Possible Promoters of Satellite DNA Array Rearrangements in Nematodes

The centromeric satellite of the wedge sole (<i>Dicologoglossa cuneata</i>, Pleuronectiformes) is composed mainly of a sequence motif conserved in other vertebrate centromeric DNAs

Centromere Evolution: Digging into Mammalian Primary Constriction

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