Molecular characterization of the gene profile of Bacillus thuringiensis Berliner isolated from Brazilian ecosystems and showing pathogenic activity against mosquito larvae of medical importance

Silva, Joelma Soares da; Queirós, Silmara Gomes; Aguiar, Jéssica Sobral de; Viana, Juliete Lima; Neta, Maria dos R.A.V.; Silva, Maria Cristina da; Pinheiro, Valéria Cristina Soares; Polanczyk, Ricardo Antônio; Carvalho‐Zilse, Gislene Almeida; Tadei, Wanderli Pedro

doi:10.1016/j.actatropica.2017.08.006

Cited by 34 publications

(39 citation statements)

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“…The gene profiles of these isolates differed from those of the strains that are already used to control mosquitoes, such as B. thuringiensis subsp. israelensis (cry4Aa, cry4Ba, cry10Aa, cry11Aa and cry11Ba), according to Soares-da- Silva et al (2017). The purpose of this study was to characterize isolates with new combinations of cry and cyt genes that are effective in the control of A. aegypti for future use in rotation with existing strains.…”

Section: Selection Of Bacillus Thuringiensis Isolatesmentioning

confidence: 99%

“…In this study, the isolates BtMA-401, BtMA-755 and BtMA-761 were characterized by polymerase chain reaction (PCR) with general primers to identify the families of genes encoding the mosquitocidal toxins: cry1, cry4, cry10, cry11, cry32, cry24, cy44Aa, cyt1 and cyt2 (Table 2). The other isolates were previously characterized by Soares-da- Silva et al (2017). The following DNA samples from Bt subspecies were used as positive controls in the reactions: B. thuringiensis subsp.…”

Section: Pcr For Amplification Of Dipteran-specific Bacillus Thuringimentioning

confidence: 99%

“…The entomopathogenic bacterium Bacillus thuringiensis (Bt), Goldberg and Margalit (1977) is considered highly effective at controlling immature forms of mosquitoes since several isolates produce δ-endotoxin-containing protein crystals with insecticidal action against several insect orders, including Diptera (Van Frankenhuyzen, 2013; Soares-da- Silva et al, 2017;Soberón et al, 2018). Bacillus thuringiensis subsp.…”

Section: Introductionmentioning

confidence: 99%

“…israelensis has insecticidal activity against the larvae of different mosquito species; thus, it is used worldwide to manufacture insecticidal formulations for vector control (Raymond et al, 2010;Bravo et al, 2011). Due to the combination of cry and cyt genes, the toxic activity of this subspecies can cause larval death 24 h after of exposure (Sun et al, 2013;El-Kersh et al, 2014;Soares-da-Silva et al, 2017).…”

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confidence: 99%

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Isolates of Bacillus thuringiensis from Maranhão biomes with potential insecticidal action against Aedes aegypti larvae (Diptera, Culicidae)

et al. 2021

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Entomopathogenic agents are viable and effective options due to their selective action against insects but benign effects on humans and the environment. The most promising entomopathogens include subspecies of Bacillus thuringiensis (Bt), which are widely used for the biological control of insects, including mosquito vectors of human pathogens. The efficacy of B. thuringiensis toxicity has led to the search for new potentially toxic isolates in different regions of the world. Therefore, soil samples from the Amazon, Cerrado and Caatinga biomes of the state of Maranhão were evaluated for their potential larvicidal action against Aedes aegypti. The isolates with high toxicity to mosquito larvae, as detected by bioassays, were subjected to histological evaluation under a light microscope to identify the genes potentially responsible for the toxicity. Additionally, the toxic effects of these isolates on the intestinal epithelium were assessed. In the new B. thuringiensis isolates toxic to A. aegypti larvae, cry and cyt genes were amplified at different frequencies, with cry4, cyt1, cry32, cry10 and cry11 being the most frequent (33-55%) among those investigated. These genes encode specific proteins toxic to dipterans and may explain the severe morphological changes in the intestine of A. aegypti larvae caused by the toxins of the isolates.

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Section: Selection Of Bacillus Thuringiensis Isolatesmentioning

confidence: 99%

Section: Pcr For Amplification Of Dipteran-specific Bacillus Thuringimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Isolates of Bacillus thuringiensis from Maranhão biomes with potential insecticidal action against Aedes aegypti larvae (Diptera, Culicidae)

et al. 2021

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“…In the last decades, the use of inactivated spores of the Bacillus thuringiensis var. israelensis (Bti) bacteria spread into the mosquito breeding places water, has accomplished desired results, reaching mortality rates above 99%[17,18]. Additionally, during the last few years, plant derived compounds have been extensively used as an alternative method for controlling mosquitoes, not only because it is a new insecticidal agent, but also because it has been described as being environmental friendly [11,19–21].…”

Section: Introductionmentioning

confidence: 99%

How long is long enough? Decreasing on plant extract lethal effects, over time, affectingAedes aegyptilarval mortality

et al. 2019

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26The mosquito Aedes aegypti has overcome all kinds of human being mosquito control attempts 27 over the last century. Strategies for vector population control resorts to the use of synthetic 28 insecticides, which can lead to problems of intoxication in humans and environmental 29 contamination. We evaluated the effects of Bacillus thuringiensis var. israelensis (Bti), Ilex 30 paraguariensis (mate-herb) and Ilex theezans (caúna-herb) extracts against A. aegypti larvae 31 mortality. The bioassays were conducted under controlled laboratory conditions of temperature 32 (27±3°C) and photoperiod (12h). Hydroalcoholic I. theezans leaves extract displayed better 33 residual effect compared to I. paraguariensis fruit aqueous extract. Variation in larval mortality 34 was also observed in the exposure periods (low after a few weeks). Low mortality after a few 35 weeks may mean increased the food for mosquito in a oppose effect over time. The residual effect 36 of Bti was observed during the 56 days of the study duration (100% of mortality). The strongest 37 residual effect of I. theezans was probably due to the presence of chemical on its leaves, such 38 coumarins, hemolytic saponins and cyanogenic glucosides, absent in I. paraguariensis. On the 39 other hand, alternative methods to vector control present risks in a long term scale by reversal of 40 larvicide effect into food resource. Our results contributed to the prospection of natural insecticides 41 and open the possibility for subsequent studies of the use of plant extracts in field situations in a 42 short time scale.43 44 Keywords: Dengue, vector control, inseticide, entomology, yerba mate. 3 3 45 Introduction 46 Over the last century the mosquito Aedes aegypti Linnaeus, 1762 has overcome all 47 mosquito control attempts by human being. Aedes females are well known by their capacity of 48 naturally and/or under laboratory conditions replicate and transmit over 100 kinds of viruses [1]. 49 As an example, in Brazil dengue, chikungunya, zika and most recently mayaro [1] viruses are a 50 real threat to public health [2]. It became necessary the A. aegypti population control in order to 51 reduce viruses' transmission and consequently the epidemic status. Although several chemical and 52 natural products have being extensively used on attempts to reduce adults and immature mosquito 53 population [3,4], appropriated mosquito population reduction is not close to come true. Especially 54 because the gene resistance selectivity due to chemicals and natural products application misuse 55[5]. Accordingly, the most effective disease prevention method still remains targeting the mosquito 56 population by elimination of mosquito breeding places [6]. 57A very promising field for mosquito population reduction is focusing the mosquito control 58 strategies on the immature aquatic stages, when the insect is more vulnerable [7,8]. For this 59 purpose, the use of synthetic insecticides is well known as being quite effective causing mosquito 60 larvae mortality [9][10][11][12][13]....

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Biosensor Characterization from Cratylia mollis Seed Lectin (Cramoll)‐MOF and Specific Carbohydrate Interactions in an Electrochemical Model

Carvalho

Araújo

Silva

et al. 2022

Chemistry & Biodiversity

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Biosensors are small devices known for their selectivity, high specificity and sensitivity to the respective analyte, at low concentrations. We developed an electrochemical biosensor using the crystalline polymer MOF-[Cu 3 (BTC) 2 (H 2 O) 2 ] n to characterize Cratylia mollis seed lectin (Cramoll) and its interaction with free carbohydrate (glucose) and carbohydrates on the surface of rabbit erythrocytes. The electrochemical potentials presented by the exponential curves that vary from 96 to 142 mV in relation to concentrations of 10 to 20 mM of glucose are decisive for the use of the system containing gold electrode/MOF/Cramoll for the characterization of biological models due to its high sensitivity. As well as the kinetic behavior presented in the cyclic voltammograms, with a cathodic current response of 0.000 3 A for a glucose concentration of 15 mM. These results were due to the high specificity of Cramoll under these conditions, promoting stability of surface charges at the Cramoll/electrode interface. This phenomenon facilitates the monitoring of the interaction with free glucose present in the electrolyte medium by potentiometric and amperometric methods and with carbohydrates present on the surface of rabbit erythrocytes through the potentiometric method. Through scanning electron microscopy (SEM) it was possible to observe Cramoll immobilized on the MOF surface, proving the specificity of the ligand (glucose-lectin) through the morphological lectin changes in this process. This electrochemical model, Cramoll/ MOF biosensor, is effective for evaluating free lectin/carbohydrate or in the erythrocyte membrane.

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Molecular characterization of the gene profile of Bacillus thuringiensis Berliner isolated from Brazilian ecosystems and showing pathogenic activity against mosquito larvae of medical importance

Cited by 34 publications

References 43 publications

Isolates of Bacillus thuringiensis from Maranhão biomes with potential insecticidal action against Aedes aegypti larvae (Diptera, Culicidae)

Isolates of Bacillus thuringiensis from Maranhão biomes with potential insecticidal action against Aedes aegypti larvae (Diptera, Culicidae)

How long is long enough? Decreasing on plant extract lethal effects, over time, affectingAedes aegyptilarval mortality

Biosensor Characterization from Cratylia mollis Seed Lectin (Cramoll)‐MOF and Specific Carbohydrate Interactions in an Electrochemical Model

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