Molecular characterization of the genome composition of partial amphiploids derived from Triticum aestivum×Thinopyrum ponticum and T. aestivum×Th. intermedium as sources of resistance to wheat streak mosaic virus and its vector, Aceria tosichella

Chen, Q.; Conner, Robert L.; Ahmad, Feroz; Laroche, André; Fedak, George; Thomas, J. B.

doi:10.1007/s001220050860

Cited by 56 publications

(56 citation statements)

References 23 publications

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“…Initially, it was thought that partial amphiploids were composed of all the wheat genomes and one complete Thinopyrum genome (Li et al 1985). However, recent molecular cytogenetic analyses have demonstrated that most of the alien genomes in the partial amphiploids examined consist of chromosomes from different genomes of Thinopyrum with a few exceptions (Banks et al 1993;Zhang et al 1996a,b;Chen et al 1998aChen et al , 2003aFedak et al 2000;Fedak and Han 2005). This conclusion was reinforced by analyzing chromosome addition lines derived from the same partial amphiploids (Chen et al 1999;Tang et al 2000).…”

Section: Transferring Resistance To Soil-borne Pathogens From Wheatgrmentioning

confidence: 58%

“…Using an St genomic probe, GISH analysis determined that Agrotana contained 8 Eor J-and 8 J s -genome-chromosomes derived from Th. ponticum (Chen et al 1998a). By crossing Chinese Spring with Agrotana, a large number of progeny lines were developed, which permitted the transfer of resistance to common root rot into wheat (Li et al 2004b).…”

Section: Transferring Resistance To Soil-borne Pathogens From Wheatgrmentioning

confidence: 99%

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Resistance to soil-borne diseases of wheat: Contributions from the wheatgrasses Thinopyrum intermedium and Th. ponticum

Li¹,

Conner²,

Murray³

2008

Can. J. Plant Sci.

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Eyespot, Cephalosporium stripe, and common root rot are soil-borne diseases that damage the stem bases, vascular system, subcrown internodes,and roots of wheat. Resistance in wheat to these diseases is insufficient to prevent significant yield loss when disease is severe. The wheatgrasses Thinopyrum intermedium and Th. ponticum are highly resistant to these diseases. Identification of disease-resistant wheat-Thinopyrum partial amphiploids, chromosome addition, substitution, and translocation lines makes them a valuable source of resistance genes for wheat breeding programs. Single chromosomes or chromosome segments containing resistance genes can be transferred into wheat to produce genetic stocks that afford a better understanding of the genetic control of resistance in wheatgrasses and new genetic resources for wheat improvement. Resistance to eyespot in Th. intermedium and Th. ponticum was associated with the homoeologous group 4 chromosomes, whereas resistance to Cephalosporium stripe was controlled by genes located on chromosomes 3 and 6 of Th. ponticum. Despite the fact that some eyespot- and common root rot-resistant wheat-Thinopyrum lines have blue kernels, resistance is not tightly linked to the blue aleurone trait. Key words: Thinopyrum intermedium, Th. ponticum, eyespot, Cephalosporium stripe, common root rot, Oculimacula yallundae, O. acuformis, Cephalosporium gramineum, Bipolaris sorokiniana

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Section: Transferring Resistance To Soil-borne Pathogens From Wheatgrmentioning

confidence: 58%

Section: Transferring Resistance To Soil-borne Pathogens From Wheatgrmentioning

confidence: 99%

Resistance to soil-borne diseases of wheat: Contributions from the wheatgrasses Thinopyrum intermedium and Th. ponticum

Li¹,

Conner²,

Murray³

2008

Can. J. Plant Sci.

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“…ponticum chromosomes of line 784, six pairs came from the J s and one from the J genome. On the other hand, Chen et al (1998b;1999a) identified eight J and eight J s chromosomes in Agrotana.…”

Section: Discussionmentioning

confidence: 99%

“…These amphiploids usually contain a complete wheat complement (42 chromosomes) and two monoploid Thinopyrum complements (14 chromosomes), but sometimes there are some substitutions of wheat chromosomes by Thinopyrum chromosomes or the opposite (Fedak et al, 2000). The genomic composition of these amphiploids has been analyzed by genomic in situ hybridization (GISH) using genomic DNA of several species as probes (Chen et al, 1998b;Cai et al, 2001). Thus, the J and J s genomes have been distinguished by the presence of sequences from the St genome (Pseudoroegneria strigosa, StSt), mainly in the centromeric regions of J s chromosomes (Chen et al, 1998a).…”

Section: Introductionmentioning

confidence: 99%

Molecular cytogenetic characterization of parental genomes in the partial amphidiploid Triticum aestivum x Thinopyrum ponticum

et al. 2005

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The wheat line PF 839197 and six hybrid derivatives from a cross between PF 839197 and Thinopyrum ponticum were cytologically characterized by fluorescent in situ hybridization (FISH). Probes for the 5S and 45S rDNA genes (pTa794 and pTa71, respectively), a highly repetitive rye sequence (pSc119.2), the synthetic oligonucleotide (AAG) 5 , and total genomic DNA from Th. ponticum and rye were used. In the wheat line, a 1RS.1BL translocation was revealed by the labeling patterns produced with pSc119.2 and (AAG) 5 , and confirmed by genomic in situ hybridization (GISH) using rye genomic DNA as a probe. Analyses of partial amphiploids confirmed previous results indicating mitotic instability, with a tendency to stabilize at 2n = 42 or 56. GISH with Th. ponticum genomic DNA showed that in one hybrid derivative, with lower chromosome numbers (2n = 42-45), chromosomes were not labeled, whereas in the hybrids with 2n = 48-56 up to 14 chromosomes were labeled. These data suggest that the original chromosome set of these hybrids was 2n = 56, and that chromosomes from both genomes were lost by mitotic instability. FISH using the rDNA probes and GISH with Thinopyrum genomic DNA suggested that cells with 2n = 56 contained an entire wheat genome plus two monoploid chromosome sets of Th. ponticum.

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“…Sheared genomic DNA from Chinese Spring wheat (ABD genomes, 2n = 42) was used as blocking DNA. Detailed procedures of chromosome preparation and hybridization mixture are described elsewhere (Chen et al, 1998). The GISH signals were detected with fluorescein-conjugated anti-digoxigenin antibodies, and the slides were mounted in Vectashield antifade solution containing propidium iodide.…”

Section: Cytogenetic Identificationmentioning

confidence: 99%

Molecular cytogenetic identification of a wheat (Triticum aestivum)-American dune grass (Leymus mollis) translocation line resistant to stripe rust

Bao

Wang²,

He³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Leymus mollis, a perennial allotetraploid (2n = 4x = 28), known as American dune grass, is a wild relative of wheat that could be useful for cultivar improvement. Shannong0096, developed from interspecific hybridization between common wheat cv. Yannong15 and L. mollis, was analyzed with cytological procedures, genomic in situ hybridization, stripe-rust resistance screening and molecular marker analysis. We found that Shannong0096 has 42 chromosomes in the root-tip cells at mitotic metaphase and 21 bivalents in the pollen mother cells at meiotic metaphase I, demonstrating cytogenetic stability. Genomic in situ hybridization probed with total genomic DNA from L. mollis gave strong hybridization signals in the distal ©FUNPEC-RP www.funpecrp.com.br Identification of a wheat-Leymus mollis translocation Genetics and Molecular Research 11 (3): 3198-3206 (2012) 3199region of two wheat chromosome arms. A single dominant Yr gene, derived from L. mollis and temporarily designated as YrSn0096, was found on the long arm of chromosome 4A of Shannong0096. YrSn0096 should be a novel Yr gene because none of the previously reported Yr genes on chromosome 4A are related to L. mollis. This gene was found to be closely linked to the loci Xbarc236 and Xksum134 with genetic distances of 5.0 and 4.8 cM, respectively. Based on data from 267 F 2 plants of Yannong15/Huixianhong, the linkage map of YrSn0096, using the two molecular markers, was established in the order Xbarc236-YrSn0096-Xksum134. Shannong0096 appeared to be a unique wheat-L. mollis translocation with cryptic alien introgression. Cytogenetic stability, a high level of stripe-rust resistance, the common wheat background, and other positive agronomic traits make it a desirable donor for introducing novel alien resistance genes in wheat breeding programs, with the advantage of molecular markers that can be used to confirm introgression.

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Molecular characterization of the genome composition of partial amphiploids derived from Triticum aestivum×Thinopyrum ponticum and T. aestivum×Th. intermedium as sources of resistance to wheat streak mosaic virus and its vector, Aceria tosichella

Cited by 56 publications

References 23 publications

Resistance to soil-borne diseases of wheat: Contributions from the wheatgrasses Thinopyrum intermedium and Th. ponticum

Resistance to soil-borne diseases of wheat: Contributions from the wheatgrasses Thinopyrum intermedium and Th. ponticum

Molecular cytogenetic characterization of parental genomes in the partial amphidiploid Triticum aestivum x Thinopyrum ponticum

Molecular cytogenetic identification of a wheat (Triticum aestivum)-American dune grass (Leymus mollis) translocation line resistant to stripe rust

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