Molecular characterization of the glycoprotein genes of H5N1 influenza A viruses isolated in Israel and the Gaza Strip during 2006 outbreaks

Shimon, Perk; Banet-Noach, Caroline; Golender, Natalia; Simanov, Lubov; Rozenblut, Ezra; Nagar, Sagit; Pokamunski, Shimon; Pirak, M.; Tendler, Y; Garcı́a, Maricarmen; Panshin, Alexander

doi:10.1007/s11262-007-0120-1

Cited by 9 publications

(8 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During recent years, the Israeli poultry industry was exposed to three of LPAI-H9N2 and one of HPAI-H5N1 [10,21]. Local H5N1 isolates were practically identical with Egyptian and other H5N1 HPAI viruses that caused Eurasian and African outbreaks in 2005-2006 [21].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic characterization of avian influenza viruses isolated in Israel during 2000–2006

Golender¹,

Panshin²,

Banet-Noach³

et al. 2008

Virus Genes

Self Cite

View full text Add to dashboard Cite

Our aim was to establish the phylogenetic and genetic relationships among avian influenza viruses (AIV) recently isolated from poultry in Israel. During this study we analyzed complete nucleotide sequences of two envelope (hemagglutinin and neuraminidase) and six internal genes (polymerase B1, polymerase B2, polymerase A, nucleoprotein, nonstructural, and matrix) of 29 selected H9N2 and six internal genes of five H5N1 viruses isolated in Israel during 2000-2006. Comparative genetic and phylogenetic analyses of these sequences revealed that the local H5N1 viruses are closely related to H5N1 viruses isolated in European, Asian, and Middle Eastern countries in 2005-2006. The H9N2 Israeli isolates, together with viruses isolated in Jordan and Saudi Arabia formed a single group. Our data support the claim that during recent years a new endemic focus of H9N2 has been formed in the Middle East. The introduction of H5N1 and co-circulation of these two subtypes of AIV in this region may augment the risk of potentially pandemic strains emergence.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The envelope protein genes of the local H5N1 AIV isolates were characterized and described previously [21].…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of avian influenza viruses isolated in Israel during 2000–2006

Golender¹,

Panshin²,

Banet-Noach³

et al. 2008

Virus Genes

Self Cite

View full text Add to dashboard Cite

show abstract

“…All flocks affected by H5N1 had been vaccinated against H9N2 infection, but evidence of co-infection with H9N2 viruses was observed in the Gaza strip region. As many as one million birds were culled to stop the spread of the H5N1 outbreak (Perk et al, 2007). …”

Section: Introductionmentioning

confidence: 99%

Modulation of the severity of highly pathogenic H5N1 influenza in chickens previously inoculated with Israeli H9N2 influenza viruses

Khalenkov¹,

Perk²,

Panshin³

et al. 2009

Virology

Self Cite

View full text Add to dashboard Cite

The continued evolution of H9N2 and H5N1 viruses and their spread and re-emergence across Eurasia raise concern that prior H9N2 virus infection may limit the detection of subsequent H5N1 infection in gallinaceous poultry by attenuating the severity of disease. We show that H9N2 viruses isolated from Israeli turkeys during 2000–2004 were antigenically and genetically distinguishable. These three H9N2 viruses caused no overt signs of disease in chickens. The 2004 isolate replicated and spread most efficiently, and chickens previously inoculated with this H9N2 virus showed 90%–100% survival after inoculation 1 to 35 days later with lethal H5N1 virus. Chickens that survived did not show signs of disease but did shed lethal H5N1 virus from the cloaca. The modulation of survivability was time-dependent; the effect was maximal five days after H9N2 inoculation. These findings suggest that co-circulation of H9N2 viruses can contribute to the spread of lethal H5N1 viruses.

show abstract

“…2). Virus A/ chicken/Gaza/833/1-10-27/2006 grouped together with two H5N1 strains isolated in Israel in 2006 and described earlier [12,20]. This virus was isolated in the Gaza Strip in 2006, from chickens infected simultaneously with both AIV subtypes: H9N2 and H5N1.…”

Section: Phylogenetic Analysismentioning

confidence: 85%

“…The other local H9N2 isolates felt into two different main groups. The first [11,[20][21][22]. Among the 230 amino acids of NS1, 164 were strongly conservative but the other 66 sites were categorized as variable.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Variability of NS1 proteins among H9N2 avian influenza viruses isolated in Israel during 2000–2009

et al. 2010

View full text Add to dashboard Cite

The main aims of the present study were to characterize NS1 protein from H9N2 avian influenza viruses (AIVs) isolated in Israel and to investigate the possibility to use NS1-based indirect ELISA. To achieve these purposes, the non-structural gene (NS1) of 79 AIVs of the H9N2 subtype isolated in Israel in 2000-2009 was sequenced and genetically analyzed. The phylogenetic analysis demonstrated that four distinct introductions of H9N2 occurred in Israel during this period. Analysis of the inferred amino acid sequences of the NS1 proteins showed high, about 10%, differences between viruses of the 3rd and 4th introductions. Antibodies against NS1 protein in immune sera were tested by means of indirect ELISA using recombinant NS1 as antigen. Immune sera were obtained from experimentally H9N2-infected chicken after infection on 4, 7, 10, 14, and 21 days. All sera from chickens experimentally infected with 3rd- or 4th-introduction AIV contained anti-NS1 antibodies that were detected by enzyme-linked immunosorbent assay (NS1-ELISA) even though the recombinant NS1 used as antigen for NS1-ELISA differed significantly in its amino acid sequences from the NS1 protein of AIV that caused infection in experimental birds. These findings indicate that the sites of the NS1 protein by which viruses belonging to 3rd and 4th introduction are out of antigenic epitope positions were responsible for the results of NS1-based iELISA.

show abstract

Molecular characterization of the glycoprotein genes of H5N1 influenza A viruses isolated in Israel and the Gaza Strip during 2006 outbreaks

Cited by 9 publications

References 48 publications

Genetic characterization of avian influenza viruses isolated in Israel during 2000–2006

Genetic characterization of avian influenza viruses isolated in Israel during 2000–2006

Modulation of the severity of highly pathogenic H5N1 influenza in chickens previously inoculated with Israeli H9N2 influenza viruses

Variability of NS1 proteins among H9N2 avian influenza viruses isolated in Israel during 2000–2009

Contact Info

Product

Resources

About