Molecular characterization of the HSPA1A gene by single-strandconformation polymorphism and sequence analysis in Holstein-Friesiancrossbred and Deoni cattle raised in India

Kerekoppa, Ramesha; Rao, A. Narasimha; Basavaraju, M.; Geetha, Gujjar Ramachandra; krishnamurthy, L; Rao, Thota V.L. Narasimha; Das, Debanand; Mukund, Kataktalware

doi:10.3906/vet-1212-3

Cited by 13 publications

(4 citation statements)

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“…Although differences in thermo tolerance at the physiological and cellular level have been documented in both Bosindicus and Bostaurus cattle (Collier et al, 2006;Chaiyabutr et al, 2008;W ilson andCrandall, 2010 andDalcin et al, 2016), data on polymorphism of HSP genes in Sahiwal cattle and Holstein Friesian crossbreds is limited. There are only a few reports from India about the relationship of HSP gene polymorphism with heat tolerance in Tharparkar cattle (Bhat et al, 2016), Deoni cattle (Kerekoppa et al, 2015), Jersey crossbred cows (Sailo et al, 2015) and from abroad in Holstein cow (Li et al, 2011). Therefore polymorphisms of HSP90AA1 gene and their association with various physiological, production and reproduction traits in Sahiwal and crossbred cows were investigated in the present study.…”

Section: Introductionmentioning

confidence: 93%

Genetic Polymorphism within Exon 3 of HSP90AA1 Gene and its Association Studies in Sahiwal and Crossbred Cows

Prasanna

Rao

Prakash

et al. 2021

AJDFR

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Background: Heat shock proteins (HSPs) are molecular chaperones that play a critical role in recovering cells from stress and form a primary system for intra cellular self defense. They are highly conserved and play a crucial role in cellular thermo tolerance and heat stress response. Though there are many HSP genes, thermo tolerance is mainly correlated with HSP70 and HSP90 genes in Livestock species. Polymorphisms in these genes have shown an association with heat tolerance, milk production, fertility and disease susceptibility in livestock. They can be used as genetic markers for the selection of animals with better climate resilience, immune response and superior performance. Methods: The present study was carried out in Sahiwal (n=50) and Crossbred cows (n=50) with the objective to identify polymorphisms in HSP90AA1 gene. A 450 bp fragment of bovine HSP90AA1 gene covering exon3 was subjected to Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique to identify the polymorphism. PCR-SSCP patterns were correlated with the physiological, productive and reproductive traits in Sahiwal and crossbred cows using the univariate GLM model of SPSS 25. Result: The PCR-SSCP of exon 3 of HSP90AA1 gene yielded two conformational patterns AA and AB corresponding to two allelic variants A and B in both Sahiwal and crossbred cows. The allele frequencies of A and B were 0.78 and 0.22 and 0.84 and 0.16 in Sahiwal and crossbred cows, respectively. The association analysis of SSCP patterns revealed that genotype AA had higher lactation length in Sahiwal cows and higher total lactation milk yield and peak yield in crossbred cows.

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Section: Introductionmentioning

confidence: 93%

Genetic Polymorphism within Exon 3 of HSP90AA1 Gene and its Association Studies in Sahiwal and Crossbred Cows

Prasanna

Rao

Prakash

et al. 2021

AJDFR

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“…Although differences in thermo tolerance at physiological and cellular level are documented (Collier et al, 2006;Chaiyabutr et al, 2008;W ilson andCrandall, 2010 andDalcin et al, 2016) in both Bos indicus and Bos taurus cattle, information on polymorphism of HSP genes in Sahiwal cattle and Holstein Friesian crossbreds is scarce. There are few reports from India regarding the association of HSP70 and HSP90 gene polymorphism with heat tolerance in Tharparkar cattle (Bhat et al, 2016), Deoni cattle (Kerekoppa et al, 2015), Jersey crossbred cows (Sailo et al, 2015) and from abroad in Holstein cow (Li et al, 2011). Therefore in the present study, polymorphisms of HSP70 and their correlation with various physiological, production and reproduction traits in Sahiwal and crossbred cows were explored.…”

Section: Introductionmentioning

confidence: 95%

Association of SSCP Polymorphisms of HSP70 Gene with Physiological, Production and Reproduction Performance in Sahiwal and Crossbred Cows

Prasanna

Rao

Prakash

et al. 2021

AJDFR

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Background: Cellular tolerance to heat stress is mediated by heat shock proteins (HSPs). The HSPs act as molecular chaperones and are transcribed in response to stress. Among different families of these proteins, HSP70 is considered to be related to the development of temperature tolerance. Unraveling polymorphism in heat shock protein genes could be a step towards the identification of genetic markers for selecting heat-tolerant cattle. Methods: The present study was carried out in Sahiwal (n=50) and Crossbred cows (n=50) with the objective to identify polymorphisms in HSP70 gene. Two fragments (295 and 220 bp) of HSP70 gene were subjected to Polymerase Chain Reaction-Single-Strand Conformation Polymorphism (PCR-SSCP) technique. Statistical analysis was performed to study the association of each SSCP genotype on physiological, production and reproduction traits in Sahiwal and crossbred cows using the univariate GLM model of SPSS 25. Result: The PCR-SSCP of 295 bp fragment of HSP70 gene revealed two genotypes AA and AB in Sahiwal cows and two genotypes AA and AC in crossbred cows. The association analysis revealed that genotype AA had higher peak milk yield in Sahiwal cows while the same genotype had higher total lactation milk yield, lower service period and calving interval in crossbred cows. The 220 bp fragment was found to be monomorphic in both Sahiwal and crossbred cows.

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“…HSP70 is classified into subfamilies based on minor structural changes in its nucleotide bases, including HSPA1A, HSPA1B, and HSPA1L. The HSPA1A gene subfamily was also associated with cattle performance (Cochran et al, 2013;Kerekoppa et al, 2015;Öner et al, 2017). Suhendro et al (2021) found a base mutation of thymine to guanine in the HSPA1A gene at 5' untranslated region (5'UTR) of transcription position -69 (g.-69T>G).…”

Section: Introductionmentioning

confidence: 99%

The Association of Single Nucleotide Polymorphism -69T>G HSPA1A Gene with Bali Cattle Heat Tolerance

Suhendro¹,

Jakaria²,

Priyanto³

et al. 2022

Trop. Anim. Sci. J.

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Heat shock protein plays an essential role in thermoregulatory during heat stress responses. This study aims to determine the association of single nucleotide polymorphism (SNP) -69T>G in the promoter region of the heat shock protein 70 member 1A (HSPA1A) gene on heat tolerance in Bali cattle. One hundred and sixteen heads of Bali cattle were collected from different locations such as Pangyangan, Bali Island; Serading, Sumbawa Island; and Sembalun, Lombok Island. The SNP was analyzed by genotyping using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), which used BstUI enzyme restriction. Physiological responses including respiration rate (Rr), rectal temperature (Tr), heart rate (Hr), heat tolerance coefficient (HTC), and blood glucose level (Glu) were measured. Association analysis was conducted using a general linear model by setting genotype, altitude, and sex as factors. The SNP -69T>G variant of HSPA1A gene found in this study were wild type (TT) with 144 bp & 498 bp; GG with 144, 236, & 262 bp; and TG with 144, 236, 262, & 498 bp. Bali cattle with the GG genotype had lower (p<0.001) Rr and HTC compared to the other genotypes. It could be concluded that physiological performances were lower at high altitudes, and the SNP -69T>G HSPA1A was associated with the physiological performances of Bali cattle. SNP -69T>G of HSPA1A could be utilized for candidate marker-assisted selection of Bali cattle to improve the performance of heat tolerance.

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Molecular characterization of the HSPA1A gene by single-strandconformation polymorphism and sequence analysis in Holstein-Friesiancrossbred and Deoni cattle raised in India

Abstract: Materials and methods Experimental animals and DNA isolationDeoni cattle are medium-sized dual-purpose Bos indicus cattle found in parts of the Maharashtra, Karnataka, and

Cited by 13 publications

References 17 publications

Genetic Polymorphism within Exon 3 of HSP90AA1 Gene and its Association Studies in Sahiwal and Crossbred Cows

Genetic Polymorphism within Exon 3 of HSP90AA1 Gene and its Association Studies in Sahiwal and Crossbred Cows

Association of SSCP Polymorphisms of HSP70 Gene with Physiological, Production and Reproduction Performance in Sahiwal and Crossbred Cows

The Association of Single Nucleotide Polymorphism -69T>G HSPA1A Gene with Bali Cattle Heat Tolerance

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Molecular characterization of the HSPA1A gene by single-strandconformation polymorphism and sequence analysis in Holstein-Friesiancrossbred and Deoni cattle raised in India

Abstract: Materials and methods Experimental animals and DNA isolationDeoni cattle are medium-sized dual-purpose Bos indicus cattle found in parts of the Maharashtra, Karnataka, and

Cited by 13 publications

References 17 publications

​Genetic Polymorphism within Exon 3 of HSP90AA1 Gene and its Association Studies in Sahiwal and Crossbred Cows

​Genetic Polymorphism within Exon 3 of HSP90AA1 Gene and its Association Studies in Sahiwal and Crossbred Cows

Association of SSCP Polymorphisms of HSP70 Gene with Physiological, Production and Reproduction Performance in Sahiwal and Crossbred Cows

The Association of Single Nucleotide Polymorphism -69T>G HSPA1A Gene with Bali Cattle Heat Tolerance

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Genetic Polymorphism within Exon 3 of HSP90AA1 Gene and its Association Studies in Sahiwal and Crossbred Cows

Genetic Polymorphism within Exon 3 of HSP90AA1 Gene and its Association Studies in Sahiwal and Crossbred Cows