Molecular characterization of the human lens epithelium-derived cell line SRA01/04

Weatherbee, Bailey A. T.; Barton, Joshua R.; Siddam, Archana D.; Anand, Deepti; Lachke, Salil A.

doi:10.1016/j.exer.2019.107787

Cited by 17 publications

(12 citation statements)

References 70 publications

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“…11 ). Our previous findings have shown that fluorescence expression intensity units of around 100 (with significant expression p-value) in the Affymetrix and Illumina microarray platforms has served as good indicators that a candidate gene will be validated by independent assays such as RT-PCR 96 , 97 .…”

Section: Methodsmentioning

confidence: 99%

A large multiethnic GWAS meta-analysis of cataract identifies new risk loci and sex-specific effects

Choquet

Melles²,

Anand

et al. 2021

Nat Commun

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Cataract is the leading cause of blindness among the elderly worldwide and cataract surgery is one of the most common operations performed in the United States. As the genetic etiology of cataract formation remains unclear, we conducted a multiethnic genome-wide association meta-analysis, combining results from the GERA and UK Biobank cohorts, and tested for replication in the 23andMe research cohort. We report 54 genome-wide significant loci, 37 of which were novel. Sex-stratified analyses identified CASP7 as an additional novel locus specific to women. We show that genes within or near 80% of the cataract-associated loci are significantly expressed and/or enriched-expressed in the mouse lens across various spatiotemporal stages as per iSyTE analysis. Furthermore, iSyTE shows 32 candidate genes in the associated loci have altered gene expression in 9 different gene perturbation mouse models of lens defects/cataract, suggesting their relevance to lens biology. Our work provides further insight into the complex genetic architecture of cataract susceptibility.

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Section: Methodsmentioning

confidence: 99%

A large multiethnic GWAS meta-analysis of cataract identifies new risk loci and sex-specific effects

Choquet

Melles²,

Anand

et al. 2021

Nat Commun

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“…Index items extracted from literature were used as cluster markers. The largest cluster #0 was marked as “causing congenital cataract-microcornea syndrome” (Huang and He, 2010 ; Hu et al, 2010 ; Jiang, 2010 ; Li et al, 2010 ) the next cluster #1 was marked as “functional snp”(Gu et al, 2016 ; Wang et al, 2016 ; Anand et al, 2018a ), the second cluster #2 was marked as “cataractous lenses” (Sousounis and Tsonis, 2012 ; Weisschuh et al, 2012 ; Deng and Yuan, 2014 ), the third cluster #3 was marked as “a1 mutation” (Zhu et al, 2010 ; Yu et al, 2012 ; Wu et al, 2017 ), the fourth cluster #4 was marked as “foxe3 mutation” (Wada et al, 2011 ; Anand et al, 2018a ; Plaisancie et al, 2018 ), cluster #5 was marked as “cell adhesion gene pvrl3” (Lachke et al, 2012 ; Weatherbee et al, 2019 ), cluster #6 was marked as “nid1 gene” (Murgiano et al, 2014 ; Osinchuk et al, 2017 ; Braun et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Emerging Trends and Research Foci in Cataract Genes: A Bibliometric and Visualized Study

Zhu

Zhang

2021

Front. Genet.

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Background: Approximately 50% of cataracts are associated with genetic factors. Genetic etiology and molecular mechanisms based on gene research increase the understanding of cataracts and provide direction for diagnosis and intervention. In the present study, SCIE papers related to the modeling of cataract gene research from 2010–2019 were evaluated and qualitative and quantitative analyses with modeling performed.Methods: The SCIE database was searched on July 6, 2021 for cataract gene publications and relevant papers published since 2010 were considered for review. Subsequently, 1,904 SCIE papers associated with cataract genes from 2010–2019 were analyzed using a bibliometric method. The publication, country, institution, journal, references, knowledgebase, keywords, and research hotspots of the papers were analyzed using an online analysis platform of literature metrology, bibliographic item co-occurrence matrix builder (BICOMB), CiteSpace V, and VOS viewer analysis tool.Results: 78 countries published the related articles, and the United States ranks of America had the most publications. Two thousand seven hundred and eighty three institutions contributed to the related publications. Fudan University had the most publications. The reference clusters of SCI papers were clustered into six categories, namely, causing congenital cataract-microcornea syndrome, functional snp, cataractous lenses, a1 mutation, foxe3 mutation, cell adhesion gene pvrl3, nid1 gene. The key words representing the research frontiers were cerebrotendinous xanthomatosis (2017-2019), oxidative stress (2017–2019).Conclusion: This study provided a systematic, objective and comprehensive analysis of the literature related to gene research of cataract. Moreover, this study demonstrated the current hotspots and the future trends in the field of gene research of cataract. This review will help ophthalmologist to discern the dynamic evolution of cataract gene research, as well as highlight areas for future research.

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“…In general, lens cell lines are more readily used than primary cultures. In the lens field, the three main lines used are HLE-B3 (Makley et al, 2015), SRA01\04 (Ibaraki et al, 1998;Weatherbee et al, 2019) and FHL124 (Dawes et al, 2007b;Reddan et al, 1999;Wormstone et al, 2004). They are all derived from foetal or neonate lenses.…”

Section: Cell Culturementioning

confidence: 99%

“…Using this system growth, EMT and differentiation have been observed and thus epithelial explants are a tractable system for the study of PCO (Gordon-Thomson et al, 1998;Mansfield et al, 2004;McAvoy and Chamberlain, 1989). Explant cultures can also be established from human lenses (Maidment et al, 2004;Shu and Lovicu, 2017;Smith et al, 2019;Weatherbee et al, 2019). To date, growth, EMT, increased matrix production and contraction have been studied, but differentiation is yet to be observed.…”

Section: In Vivo Animal Modelsmentioning

confidence: 99%

Posterior capsule opacification: What's in the bag?

Wormstone

Smith

et al. 2021

Progress in Retinal and Eye Research

120

102

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Molecular characterization of the human lens epithelium-derived cell line SRA01/04

Cited by 17 publications

References 70 publications

A large multiethnic GWAS meta-analysis of cataract identifies new risk loci and sex-specific effects

A large multiethnic GWAS meta-analysis of cataract identifies new risk loci and sex-specific effects

Emerging Trends and Research Foci in Cataract Genes: A Bibliometric and Visualized Study

Posterior capsule opacification: What's in the bag?

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