Molecular characterization of the human ovulatory cascade—Lesson from the IVF/IVM model

Yerushalmi, Gil M.; Maman, Ettie; Yung, Yuval; Kedem, Alon; Hourvitz, Ariel

doi:10.1007/s10815-011-9594-9

Cited by 10 publications

(7 citation statements)

References 38 publications

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“…Although in vitro culture (IVC) systems are applied to mimic in vivo conditions, the proportion of fully mature and fertilizable oocytes after IVC in numerous mammalian species is still unsatisfactory [ 28 ]. Recently, several studies aimed to find new molecular markers of oocyte maturation ability in order to improve IVC models and increase the number of fully mature gametes [ 29 – 33 ]. Therefore, in this study we performed gene expression analysis in porcine oocytes before and after IVM in order to define new molecular markers of female gamete maturation capability.…”

Section: Discussionmentioning

confidence: 99%

Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM – a microarray approach

Budna

Rybska

Ciesiółka

et al. 2017

Reprod Biol Endocrinol

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BackgroundThe full maturational capability of mammalian oocytes is accompanied by nuclear and cytoplasmic modifications, which are associated with proliferation and differentiation of surrounding cumulus cells. These events are regulated on molecular level by the expression of target genes involved in signal transduction pathways crucial for folliculogenesis and oogenesis. Transforming growth factor beta signaling includes several molecules that are involved in the regulation of oogenesis and embryo growth, including bone morphogenetic protein (BMP). However, the BMP-related gene expression profile in oocytes at different maturational stages requires further investigation.MethodsOocytes were isolated from pubertal crossbred Landrace gilts follicles, selected with a use of BCB staining test and analyzed before and after in vitro maturation. Gene expression profiles were examined using an Affymetrix microarray approach and validated by RT-qPCR. Database for Annotation, Visualization, and Integrated Discovery (DAVID) software was used for the extraction of the genes belonging to a BMP-signaling pathway ontology group.ResultsThe assay revealed 12,258 different transcripts in porcine oocytes, among which 379 genes were down-regulated and 40 were up-regulated. The DAVID database indicated a “BMP signaling pathway” ontology group, which was significantly regulated in both groups of oocytes. We discovered five up-regulated genes in oocytes before versus after in vitro maturation (IVM): chordin-like 1 (CHRDL1), follistatin (FST), transforming growth factor-beta receptor-type III (TGFβR3), decapentaplegic homolog 4 (SMAD4), and inhibitor of DNA binding 1 (ID1).ConclusionsIncreased expression of CHRDL1, FST, TGFβR3, SMAD4, and ID1 transcripts before IVM suggested a subordinate role of the BMP signaling pathway in porcine oocyte maturational competence. Conversely, it is postulated that these genes are involved in early stages of folliculogenesis and oogenesis regulation in pigs, since in oocytes before IVM increased expression was observed.

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Section: Discussionmentioning

confidence: 99%

Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM – a microarray approach

Budna

Rybska

Ciesiółka

et al. 2017

Reprod Biol Endocrinol

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“…As we showed previously [ 8 , 9 , 10 , 11 , 23 ], transcriptomic profile of gene expression differs significantly in porcine oocytes before and after IVM. Thus, to improve the quantity of mature and fertilizable oocytes, as well as IVF outcome, finding new molecular markers of maturation capability is highly recommended [ 24 , 25 , 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Significant Down-Regulation of “Biological Adhesion” Genes in Porcine Oocytes after IVM

Budna

Celichowski

Bryja

et al. 2017

IJMS

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Proper maturation of the mammalian oocyte is a compound processes determining successful monospermic fertilization, however the number of fully mature porcine oocytes is still unsatisfactory. Since oocytes’ maturation and fertilization involve cellular adhesion and membranous contact, the aim was to investigate cell adhesion ontology group in porcine oocytes. The oocytes were collected from ovaries of 45 pubertal crossbred Landrace gilts and subjected to two BCB tests. After the first test, only granulosa cell-free BCB+ oocytes were directly exposed to microarray assays and RT-qPCR (“before IVM” group), or first in vitro matured and then if classified as BCB+ passed to molecular analyses (“after IVM” group). As a result, we have discovered substantial down-regulation of genes involved in adhesion processes, such as: organization of actin cytoskeleton, migration, proliferation, differentiation, apoptosis, survival or angiogenesis in porcine oocytes after IVM, compared to oocytes analyzed before IVM. In conclusion, we found that biological adhesion may be recognized as the process involved in porcine oocytes’ successful IVM. Down-regulation of genes included in this ontology group in immature oocytes after IVM points to their unique function in oocyte’s achievement of fully mature stages. Thus, results indicated new molecular markers involved in porcine oocyte IVM, displaying essential roles in biological adhesion processes.

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“…At present, during IVM, nuclear maturation occurs in 50-70% of oocytes, but cytoplasmic maturation is disturbed, leading to asynchrony between the cytoplasmic and nucleus compartments. There have been extensive efforts to elucidate the mechanisms that are responsible for the difference in the efficacy of in vitro and IVM (Sirard, 2011;Yerushalmi et al, 2011;Nogueira et al, 2012). The study of the cumulus granulosa cell (GC) transcriptome has been proposed as a research approach with potential to lead to an improvement in the efficiency of IVM.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation

Yerushalmi

Salmon‐Divon

Yung

et al. 2014

MHR: Basic Science of Reproductive Medicine

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Cumulus expansion and oocyte maturation are central processes in ovulation. Knowledge gained from rodent and other mammalian models has revealed some of the molecular pathways associated with these processes. However, the equivalent pathways in humans have not been thoroughly studied and remain unidentified. Compact cumulus cells (CCs) from germinal vesicle cumulus oocyte complexes (COCs) were obtained from patients undergoing in vitro maturation (IVM) procedures. Expanded CCs from metaphase 2 COC were obtained from patients undergoing IVF/ICSI. Global transcriptome profiles of the samples were obtained using state-of-the-art RNA sequencing techniques. We identified 1746 differentially expressed (DE) genes between compact and expanded CCs. Most of these genes were involved in cellular growth and proliferation, cellular movement, cell cycle, cell-to-cell signaling and interaction, extracellular matrix and steroidogenesis. Out of the DE genes, we found 89 long noncoding RNAs, of which 12 are encoded within introns of genes known to be involved in granulosa cell processes. This suggests that unique noncoding RNA transcripts may contribute to the regulation of cumulus expansion and oocyte maturation. Using global transcriptome sequencing, we were able to generate a library of genes regulated during cumulus expansion and oocyte maturation processes. Analysis of these genes allowed us to identify important new genes and noncoding RNAs potentially involved in COC maturation and cumulus expansion. These results may increase our understanding of the process of oocyte maturation and could ultimately improve the efficacy of IVM treatment.

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Molecular characterization of the human ovulatory cascade—Lesson from the IVF/IVM model

Cited by 10 publications

References 38 publications

Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM – a microarray approach

Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM – a microarray approach

Significant Down-Regulation of “Biological Adhesion” Genes in Porcine Oocytes after IVM

Characterization of the human cumulus cell transcriptome during final follicular maturation and ovulation

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