Molecular Characterization of the
            <i>Escherichia coli</i>
            Asymptomatic Bacteriuria Strain 83972: the Taming of a Pathogen

Klemm, Per; Roos, Viktoria; Ulett, Glen C.; Svanborg, Catharina; Schembri, Mark A.

doi:10.1128/iai.74.1.781-785.2006

Cited by 117 publications

(130 citation statements)

References 39 publications

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“…E. coli strains belonging to group B2 are associated with pyelonephritis and other extraintestinal invasive clinical syndromes, such as bacteremia, prostatitis, and meningitis. Also, we recently characterized the fim, pap, and foc gene clusters of the strain and showed that although the fimbriae encoded by these clusters are nonfunctional, the strain contains sequence information that bears witness of a pathogenic past (26,41). Thus, the fimH allele of 83972 encodes minor amino acid variations (compared with the K-12 version) that are consistent with those of previously characterized pyelonephritis isolates (51)(52)(53).…”

supporting

confidence: 55%

Global Gene Expression Profiling of the Asymptomatic Bacteriuria Escherichia coli Strain 83972 in the Human Urinary Tract

Roos

Klemm

2006

Infect Immun

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Urinary tract infections (UTIs) are an important health problem worldwide, with many million cases each year. Escherichia coli is the most common organism causing UTIs in humans. The asymptomatic bacteriuria E. coli strain 83972 is an excellent colonizer of the human urinary tract, where it causes long-term bladder colonization. The strain has been used for prophylactic purposes in patients prone to more severe and recurrent UTIs. For this study, we used DNA microarrays to monitor the expression profile of strain 83972 in the human urinary tract. Significant differences in expression levels were seen between the in vivo expression profiles of strain 83972 in three patients and the corresponding in vitro expression profiles in lab medium and human urine. The data revealed an in vivo lifestyle of microaerobic growth with respiration of nitrate coupled to degradation of sugar acids and amino acids, with no signs of attachment to host tissues. Interestingly, genes involved in NO protection and metabolism showed significant up-regulation in the patients. This is one of the first studies to address bacterial whole-genome expression in humans and the first study to investigate global gene expression of an E. coli strain in the human urinary tract.

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supporting

confidence: 55%

Global Gene Expression Profiling of the Asymptomatic Bacteriuria Escherichia coli Strain 83972 in the Human Urinary Tract

Roos

Klemm

2006

Infect Immun

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“…In this regard, Svanborg and colleagues have recently proposed an interesting mechanism to explain the occurrence of ASB where Toll-like receptor 4 (TLR4) controls the initial mucosal response to UPEC, suggesting that the development of symptomatic infection would be more closely associated with variation of the host environment than with expression of bacterial VFs, since TLR4 mutant mice develop an asymptomatic carrier state resembling human ASB (20). They have shown that a clinical ASB-associated strain, E. coli 83972, is able to colonize the human urinary bladder without inducing an immune response, through the mutation of its genes encoding type 1 and P fimbriae (14). Thus, the development of symptomatic infection may be more closely associated with the variation in the host environment than the virulence of the microorganisms.…”

mentioning

confidence: 99%

Escherichia coli Isolates Associated with Uncomplicated and Complicated Cystitis and Asymptomatic Bacteriuria Possess Similar Phylogenies, Virulence Genes, and O-Serogroup Profiles

et al. 2006

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The genetic and serological characteristics of Escherichia coli isolates from patients with uncomplicated cystitis (UC), complicated cystitis (CC), and complicated asymptomatic bacteriuria (CASB) were determined. Phylogenetic group B2 was predominant in all categories. The prevalences of 14 out of 18 virulence factor genes were similar among the three categories, while pap, iha, ompT, and PAI were more frequently seen in isolates associated with UC than CC or CASB.

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“…Microorganisms use various mechanisms to acquire drug resistance viz. horizontal gene transfer through plasmid, transposons and bacteriophage, recombination of foreign DNA in bacterial chromosome, and mutation in different chromosomal locus (Klemm et al, 2006). Brown et al (2003) have reported that the horizontal gene transfer is the factor in occurrence of antibiotic resistance in clinical isolates and suggested that the high prevalence of resistance to a particular antibiotic does not always reflect antibiotic consumption as suggested by Nwanze et al (2007).…”

Section: Mechanism Of Microbial Resistance Towards Antimicrobial Agentmentioning

confidence: 95%

Resistance by Enterobacter spp. towards several antimicrobial drugs and heavy metals: A review

M¹,

Bagde²

2017

Afr. J. Biotechnol.

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Antimicrobial resistance developed in several pathogens poses an increasing threat to human health across the world. No country can escape from the medical and economic impacts from this serious problem. Although the antibiotic resistance is not a new phenomenon, the current magnitude and speed with which it is developing is a cause for the global concern including in India. There are so many common diseases resulting from the microorganisms such as blood stream infections, urinary tract infections, post-operative wound infections and intra-abdominal infections. In this review the antimicrobial susceptibility or resistance of Enterobacter towards antimicrobial agents and heavy metals, viz. ceftazidime, moxifloxacin, nalidixic acid, sulfamethaxazole, and nickel and lead is discussed briefly along with other antimicrobials and heavy metals. The mechanisms behind the resistance by Enterobacter was analyzed and evaluated by many workers after using currently employed susceptibility testing methods for Enterobacter spp. There are some factors influencing mode of action of fluoroquinolones, quinolones and sulfamethaxazole. History, classification, identification, clinical features and treatment of infections and the epidemiology of antimicrobials (drugs and heavy metals) resistance by the Enterobacter spp. is included in this review. Now a day, antimicrobial resistance is common in hospitals where acquired infections can be perilous. This situation compels scientists to synthesize new antibiotics and treatment modalities. Enterobacter causes nosocomial infections. It is ubiquitous and can survive on skin and dry surfaces and replicate in contaminated fluids. Numerous outbreaks have been described. Various mechanisms have been adapted by microorganisms to resist toxicity of antimicrobials. Antimicrobial drugs may be rendered inactive or ineffective by the major ways such as barrier to antibiotic entry into the bacterial cell, prevention of the antibiotic from reaching the target, often by extrusion, alteration of the target of the drug and inactivation of the antibiotic by modification or destruction. In addition, bacteria may be able to bypass the metabolic pathway affected by a particular drug or may be able to overproduce an enzyme that is inhibited by the drug action, more than one mechanism may operate at any given time

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Molecular Characterization of the Escherichia coli Asymptomatic Bacteriuria Strain 83972: the Taming of a Pathogen

Cited by 117 publications

References 39 publications

Global Gene Expression Profiling of the Asymptomatic Bacteriuria Escherichia coli Strain 83972 in the Human Urinary Tract

Global Gene Expression Profiling of the Asymptomatic Bacteriuria Escherichia coli Strain 83972 in the Human Urinary Tract

Escherichia coli Isolates Associated with Uncomplicated and Complicated Cystitis and Asymptomatic Bacteriuria Possess Similar Phylogenies, Virulence Genes, and O-Serogroup Profiles

Resistance by Enterobacter spp. towards several antimicrobial drugs and heavy metals: A review

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