Molecular Characterization of the<i>ZKT</i>Gene Encoding a Protein with PDZ, K-Box, and TPR Motifs in<i>Arabidopsis</i>

Ishikawa, Atsushi; Tanaka, Hideaki; Kato, Chiyuki; Iwasaki, Yukimoto; Asahi, Tadashi

doi:10.1271/bbb.69.972

Cited by 19 publications

(15 citation statements)

References 18 publications

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“…The abundance of two chloroplast proteins of unknown function ( Table 3 ) varied in a genotype-dependent manner. Spots 122 and 183 corresponded to a protein containing a tetratricopeptide repeat (TPR) region based on protein sequence analysis ( Ishikawa et al , 2005 ). Spot 200 was identified as a thylakoid lumen protein of 19kDa (TL19) ( Schubert et al , 2002 ).…”

Section: Resultsmentioning

confidence: 99%

An Arabidopsis soluble chloroplast proteomic analysis reveals the participation of the Executer pathway in response to increased light conditions

Uberegui

Hall

Lorenzo³

et al. 2015

Journal of Experimental Botany

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Section: Resultsmentioning

confidence: 99%

An Arabidopsis soluble chloroplast proteomic analysis reveals the participation of the Executer pathway in response to increased light conditions

Uberegui

Hall

Lorenzo³

et al. 2015

Journal of Experimental Botany

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“…Previous studies in animals have shown that the proteins rich in the PDZ domain usually act as key nodes in complex protein—protein interaction networks [ 42 , 43 ]. In plants, the ZKT protein acts as a molecular adaptor that is regulated by phosphorylation in the wound response [ 44 ]. Zhu et al .…”

Section: Discussionmentioning

confidence: 99%

Elucidation of Cross-Talk and Specificity of Early Response Mechanisms to Salt and PEG-Simulated Drought Stresses in Brassica napus Using Comparative Proteomic Analysis

et al. 2015

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To understand the cross-talk and specificity of the early responses of plants to salt and drought, we performed physiological and proteome analyses of Brassica napus seedlings pretreated with 245 mM NaCl or 25% polyethylene glycol (PEG) 6000 under identical osmotic pressure (-1.0 MPa). Significant decreases in water content and photosynthetic rate and excessive accumulation of compatible osmolytes and oxidative damage were observed in response to both stresses. Unexpectedly, the drought response was more severe than the salt response. We further identified 45 common differentially expressed proteins (DEPs), 143 salt-specific DEPs and 160 drought-specific DEPs by isobaric tags for relative and absolute quantitation (iTRAQ) analysis. The proteome quantitative data were then confirmed by multiple reaction monitoring (MRM). The differences in the proteomic profiles between drought-treated and salt-treated seedlings exceeded the similarities in the early stress responses. Signal perception and transduction, transport and membrane trafficking, and photosynthesis-related proteins were enriched as part of the molecular cross-talk and specificity mechanism in the early responses to the two abiotic stresses. The Ca2+ signaling, G protein-related signaling, 14-3-3 signaling pathway and phosphorylation cascades were the common signal transduction pathways shared by both salt and drought stress responses; however, the proteins with executive functions varied. These results indicate functional specialization of family proteins in response to different stresses, i.e., CDPK21, TPR, and CTR1 specific to phosphorylation cascades under early salt stress, whereas STN7 and BSL were specific to phosphorylation cascades under early drought stress. Only the calcium-binding EF-hand family protein and ZKT were clearly identified as signaling proteins that acted as cross-talk nodes for salt and drought signaling pathways. Our study provides new clues and insights for developing strategies to improve the tolerance of crops to complex, multiple environmental stresses.

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“…A homology model for mature Arabidopsis MET1 shows the PDZ and TPR domains, whereas the highly conserved C terminus is rich in hydrophobic residues and forms a number of short helices (Supplemental Figure 2) (see Discussion). Arabidopsis MET1 was previously assigned as a ZKT protein (for PDZ, K-BOX, and TPR motifs), but neither protein location nor function was determined (Ishikawa et al, 2005), and it does not appear that MET1 contains a genuine K-BOX domain. In the remainder of the article, we determine the function of the Arabidopsis MET1 protein and discuss its significance in chloroplast homeostasis.…”

Section: Met1 Is Conserved Across Photosynthetic Eukaryotesmentioning

confidence: 99%

MET1 Is a Thylakoid-Associated TPR Protein Involved in Photosystem II Supercomplex Formation and Repair in Arabidopsis

et al. 2015

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ORCID ID: 0000-0001-9536-0487 (K.J.v.W.)Photosystem II (PSII) requires constant disassembly and reassembly to accommodate replacement of the D1 protein. Here, we characterize Arabidopsis thaliana MET1, a PSII assembly factor with PDZ and TPR domains. The maize (Zea mays) MET1 homolog is enriched in mesophyll chloroplasts compared with bundle sheath chloroplasts, and MET1 mRNA and protein levels increase during leaf development concomitant with the thylakoid machinery. MET1 is conserved in C3 and C4 plants and green algae but is not found in prokaryotes. Arabidopsis MET1 is a peripheral thylakoid protein enriched in stroma lamellae and is also present in grana. Split-ubiquitin assays and coimmunoprecipitations showed interaction of MET1 with stromal loops of PSII core components CP43 and CP47. From native gels, we inferred that MET1 associates with PSII subcomplexes formed during the PSII repair cycle. When grown under fluctuating light intensities, the Arabidopsis MET1 null mutant (met1) showed conditional reduced growth, near complete blockage in PSII supercomplex formation, and concomitant increase of unassembled CP43. Growth of met1 in high light resulted in loss of PSII supercomplexes and accelerated D1 degradation. We propose that MET1 functions as a CP43/CP47 chaperone on the stromal side of the membrane during PSII assembly and repair. This function is consistent with the observed differential MET1 accumulation across dimorphic maize chloroplasts.

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Molecular Characterization of theZKTGene Encoding a Protein with PDZ, K-Box, and TPR Motifs inArabidopsis

Cited by 19 publications

References 18 publications

An Arabidopsis soluble chloroplast proteomic analysis reveals the participation of the Executer pathway in response to increased light conditions

An Arabidopsis soluble chloroplast proteomic analysis reveals the participation of the Executer pathway in response to increased light conditions

Elucidation of Cross-Talk and Specificity of Early Response Mechanisms to Salt and PEG-Simulated Drought Stresses in Brassica napus Using Comparative Proteomic Analysis

MET1 Is a Thylakoid-Associated TPR Protein Involved in Photosystem II Supercomplex Formation and Repair in Arabidopsis

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