Molecular characterization of the lgals1 gene in large scale loach Paramisgurnus dabryanus

Zhou, Bolan; Long, Yong; Song, Guangliang; Li, Qing; Cui, Zongbin

doi:10.1016/j.gene.2015.11.025

Cited by 6 publications

(5 citation statements)

References 50 publications

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“…Due to its rich nutrition, delicate meat, delicious taste, P. dabryanus is known as “ginseng in the water.” As a small freshwater benthic fish, it is loved by consumers and has a huge market potential. At present, there have been related reports on genetics and breeding (Zhou, Long, Song, Li, & Cui, 2016; Zhu & Liu, 2016), nutritional requirement (Gao et al., 2014; Wang, Hu, Cao, Yang, & Wang, 2007) and immunoregulation (Victor et al., 2019; Zhang, 2019; Zhu & Wu, 2018) of loach. However, the rough artificial feeding technology (no nutrition need standard, no commercial artificial feed) and high stocking density have resulted in negative outcomes, such as feed waste, aquaculture water environment deterioration, increasing incidence of stress and disease, seriously affecting the healthy growth of loach, reducing the product quality and economic benefits (Gao et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary astaxanthin on the growth, innate immunity and antioxidant defence system of Paramisgurnus dabryanus

Chen

Gao

et al. 2020

Aquacult. Nutr.

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The effects of astaxanthin supplementationon the growth, innate immunity and antioxidant defence system of loach (Paramisgurnus dabryanus) were investigated in this study. A total of 450 fish (initial average weights of 3.00 ± 0.10 g) were fed five diets with graded levels of astaxanthin (0.00, 50.00, 100.00, 150.00 and 200.00 mg/kg) for 56 days. The results showed that astaxanthin supplementation significantly stimulated the growth (FBW, WG, FER, HSI), innate immunity (AKP activity in the hepatopancreas, intestinal tract, muscle and skin and LZM activity in the hepatopancreas, intestinal tract and skin), antioxidant ability (T‐SOD, GSH‐PX and CAT activities and T‐AOC, GSH and MDA contents in the hepatopancreas, intestinal tract, muscle and skin) of loach. Moreover, dietary astaxanthin supplementation significantly up‐regulated the relative mRNA levels of Nrf2 and Maf, and down‐regulated the relative mRNA level of Keap1 in the hepatopancreas when the supplementation levels of astaxanthin were 50–200 mg/kg. In conclusion, the diets with 100–151.06 mg/kg astaxanthin supplementation were optimal for loach, which based on the growth, immunity and antioxidant‐related indicators, and the astaxanthin supplementation regulated the antioxidant ability partially referring to Keap1‐Nrf2 signallings.

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Section: Introductionmentioning

confidence: 99%

Effects of dietary astaxanthin on the growth, innate immunity and antioxidant defence system of Paramisgurnus dabryanus

Chen

Gao

et al. 2020

Aquacult. Nutr.

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“…Snail and Twist) [ 39 ]. Moreover, another study identified a potential binding site for NF-κB in the basal promoter of Galectin-1, suggesting that NF-κBwas also associated with Galectin-1 regulation [ 40 ]. Galectin-1 was also recently shown to promote tumor progression through upregulation of CXCR4 via activation of the NF-κB pathway [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

PSC-derived Galectin-1 inducing epithelial-mesenchymal transition of pancreatic ductal adenocarcinoma cells by activating the NF-κB pathway

et al. 2017

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Galectin-1 has previously been shown to be strongly expressed in activated pancreatic stellate cells (PSCs) and promote the development and metastasis of pancreatic ductal adenocarcinoma (PDAC). However, the molecular mechanisms by which Galectin-1 promotes the malignant behavior of pancreatic cancer cells remain unclear. In this study, we examined the effects of Galectin-1 knockdown or overexpression in PSCs co-cultured with pancreatic cancer (PANC-1) cells. Immunohistochemical analysis showed expression of epithelial-mesenchymal transition (EMT) markers and MMP9 were positively associated with the expression of Galectin-1 in 66 human PDAC tissues. In addition, our in vitro studies showed PSC-derived Galectin-1 promoted the proliferation, invasion, and survival (anti-apoptotic effects) of PANC-1 cells. We also showed PSC-derived Galectin-1 induced EMT of PANC-1 cells and activated the NF-кB pathway in vitro. Our mixed (PSCs and PANC-1 cells) mouse orthotopic xenograft model indicated that overexpression of Galectin-1 in PSCs significantly promoted the proliferation, growth, invasion, and liver metastasis of the transplanted tumor. Moreover, Galectin-1 overexpression in PSCs was strongly associated with increased expression of EMT markers in both the orthotopic xenograft tumor in the pancreas and in metastatic lesions of naked mice. We conclude that PSC-derived Galectin-1 promotes the malignant behavior of PDAC by inducing EMT via activation of the NF-κB pathway. Our results suggest that targeting Galectin-1 in PSCs could represent a promising therapeutic strategy for PDAC progression and metastasis.

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“…Genome walking from both arms of the transposon was performed as previously reported [ 64 , 65 , 66 ] to clone the flanking sequences and to determine the genomic integration site of the transgene. The genomic DNA of the F1 individual that was revealed to carry a single copy of transgene by both qPCR analysis and Southern blotting was used as the template for the PCR assays.…”

Section: Methodsmentioning

confidence: 99%

Generation of GCaMP6s-Expressing Zebrafish to Monitor Spatiotemporal Dynamics of Calcium Signaling Elicited by Heat Stress

Long

Xie

et al. 2021

IJMS

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The ability of organisms to quickly sense and transduce signals of environmental stresses is critical for their survival. Ca2+ is a versatile intracellular messenger involved in sensing a wide variety of stresses and regulating the subsequent cellular responses. So far, our understanding for calcium signaling was mostly obtained from ex vivo tissues and cultured cell lines, and the in vivo spatiotemporal dynamics of stress-triggered calcium signaling in a vertebrate remains to be characterized. Here, we describe the generation and characterization of a transgenic zebrafish line with ubiquitous expression of GCaMP6s, a genetically encoded calcium indicator (GECI). We developed a method to investigate the spatiotemporal patterns of Ca2+ events induced by heat stress. Exposure to heat stress elicited immediate and transient calcium signaling in developing zebrafish. Cells extensively distributed in the integument of the head and body trunk were the first batch of responders and different cell populations demonstrated distinct response patterns upon heat stress. Activity of the heat stress-induced calcium signaling peaked at 30 sec and swiftly decreased to near the basal level at 120 sec after the beginning of exposure. Inhibition of the heat-induced calcium signaling by LaCl3 and capsazepine and treatment with the inhibitors for CaMKII (Ca²⁺/calmodulin-dependent protein kinase II) and HSF1 (Heat shock factor 1) all significantly depressed the enhanced heat shock response (HSR). Together, we delineated the spatiotemporal dynamics of heat-induced calcium signaling and confirmed functions of the Ca2+-CaMKII-HSF1 pathway in regulating the HSR in zebrafish.

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Molecular characterization of the lgals1 gene in large scale loach Paramisgurnus dabryanus

Cited by 6 publications

References 50 publications

Effects of dietary astaxanthin on the growth, innate immunity and antioxidant defence system of Paramisgurnus dabryanus

Effects of dietary astaxanthin on the growth, innate immunity and antioxidant defence system of Paramisgurnus dabryanus

PSC-derived Galectin-1 inducing epithelial-mesenchymal transition of pancreatic ductal adenocarcinoma cells by activating the NF-κB pathway

Generation of GCaMP6s-Expressing Zebrafish to Monitor Spatiotemporal Dynamics of Calcium Signaling Elicited by Heat Stress

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