Molecular characterization of the pathogenic plant fungus Rhizoctonia solani (Ceratobasidiaceae) isolated from Egypt based on protein and PCR-RAPD profiles

Abstract

ABSTRACT. Twenty-one isolates of Rhizoctonia solani were categorized into three anastomosis groups consisting of AG-4-HG-I (eight isolates), AG-2-2 (nine isolates) and AG-5 (four isolates). Their pathogenic capacities were tested on cotton cultivar Giza 86. Pre-emergence damping-off varied in response to the different isolates; however, the differences were not significant. Soluble proteins of the fungal isolates were electrophoresed using SDS-PAGE and gel electrophoreses. A dendrogram of the protein banding patterns by the UPGMA of arithmetic means placed the fungal isolates into distinct groups. There was no evidence of a relationship between protein dendrogram, anastomosis grouping or level of virulence or geographic origin. The dendrogram generated from these isolates based on PCR analysis with five RAPD-PCR primers showed high levels of genetic similarity among the isolates from the same geographical locations. There was partially relationship between the genetic similarity and AGs or level of virulence or geographic origin based on RAPD dendrogram. These results demonstrate that RAPD technique is a useful tool in determining the genetic characterization among isolates of R. solani.

Genetic diversity and virulence spectrum of Rhizoctonia solani, the incitant of banded leaf and sheath blight of maize

“…Out of these, group Ia (Rsl-1, Rsl-43 and Rsl-44) isolates were collected from Zone-V and group IIa isolates (Rsh-3 and Rss-12) were collected from Zone-II. These results are in accordance with earlier studies conducted by Singh et al (2002), Guleria et al (2007, and Mahmoud et al (2012) where partial relationship was observed between level of virulence and geographic origin based on RAPD profiles.…”

“…(2007), and Mahmoud et al. (2012) where partial relationship was observed between level of virulence and geographic origin based on RAPD profiles.…”

Molecular characterization of Aspergillus flavus and aflatoxin contamination of wheat grains from Saudi Arabia

“…Twenty-one isolates of Rhizoctonia solani were characterized by RAPD-PCR. There was a partial relationship between GS and anastomosis groups or level of virulence or geographic origin on the basis of the RAPD dendrogram (Mahmoud et al, 2012).…”

Research Article Genetic variability of <i>Aspergillus</i> <i>flavus</i> isolated from commercial peanut and bulgur wheat in Southern Brazil and antifungal activity of essential oils against some of the isolates

“…The sequences were compared with others deposited in databanks for species definition. Mahmoud et al, 2012Mahmoud et al, 2012Mahmoud et al, 2012Batista et al, 2008Batista et al, 2008Batista et al, 2008Batista et al, 2008Batista et al, 2008Batista et al, 2008 (GACA)4 (AGAG)4G (GTG)5 5´-GACAGACAGACAGACA 5´-AGAGAGAGAGAGAGAGG 5´-GTGGTGGTGGTGGTG Batista et al, 2008Batista et al, 2008Hatti et al, 2010 Figure 1. Phylogenetic analysis of the 5.8S-ITS and -tubulin gene sequences from the Aspergillus flavus isolates.…”