Molecular characterization of Theileria equi in horses from the state of Rio de Janeiro, Brazil

Peckle, Maristela; Pires, Marcus Sandes; Silva, Cláudia Bezerra da; Costa, Renata Lins da; Vitari, Gabriela Lopes Vivas; Senra, Marcus Vinicius Xavier; Dias, Roberto Júnio Pedroso; Santos, Huarrisson Azevedo; Massard, Carlos Luiz

doi:10.1016/j.ttbdis.2017.11.011

Cited by 22 publications

(13 citation statements)

References 28 publications

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“…Theileria equi clade E has been notably linked to Asian countries (Alanazi et al, 2014;Munkhjargal et al, 2013;Tian et al, 2013), but has also previously been described in Europe (Liu et al, 2016). A different situation is observed in the Americas, where a predominance of clade A isolates, and absence of clade E isolates, has been reported in studies carried out in North America (Hall et al, 2013) and Brazil (Peckle et al, 2018). An absence of clade E isolates has also been reported in southern and western Africa (Bhoora et al, 2009;Coultous et al, 2019b) and the Middle-East (Ketter-Ratzon et al, 2017).…”

Section: Discussionmentioning

confidence: 84%

Phylogenetic analysis and geographical distribution of Theileria equi and Babesia caballi sequences from horses residing in Spain

Camino

Cruz-López

Juan

et al. 2020

Ticks and Tick-borne Diseases

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Section: Discussionmentioning

confidence: 84%

Phylogenetic analysis and geographical distribution of Theileria equi and Babesia caballi sequences from horses residing in Spain

Camino

Cruz-López

Juan

et al. 2020

Ticks and Tick-borne Diseases

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“…In contrast, previous studies have shown higher genetic diversity within 18S rRNA sequences from T. equi in horses from Brazil [29], Jordan [62], South Africa [37], and Switzerland. A low diversity within T. equi sequences from Chile could be related to the history of horses' arrival in the country, and the equid business movement trends [63]. Consistent with the above, although Chile has one of the most prestigious racecourses in Latin America [60], it is a minor participant in the global equine industry, and engages in less movement of horses [64], which in turn might have contributed to the low T. equi genetic diversity observed in the present study.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and Genetic Diversity of Babesia caballi and Theileria equi in Chilean Thoroughbred Racing Horses

et al. 2021

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This study aimed to serologically and molecularly survey Babesia caballi and Theileria equi in thoroughbred horses from racecourses in Chile. Additionally, the genetic diversity of the positive samples was assessed. A total of 286 thoroughbred horses from the Santiago and Valparaíso racecourses had their serum samples submitted to an ELISA for B. caballi and T. equi, and 457 samples (from the Santiago, Valparaíso, and Concepción racecourses) were tested with nested PCRs for the B. caballi 48 KDa rhoptry protein (RAP-1) and T. equi 18S rRNA genes. Selected RAP-1 and 18S positive products were sequenced to perform phylogenetic and haplotype analyses. An overall seroprevalence of 35.6% was observed for these Chilean racecourses: 23.7% for T. equi, 8.4% for B. caballi, and 3.5% for both agents. Overall, a 53.6% occurrence by nPCR was detected for the three Chilean racecourses: 44.2% for T. equi, 5.4% for B. caballi, and 3.9% for both agents. Phylogenetic analysis of T. equi and B. caballi showed genetic proximity with sequences previously detected in other countries. Haplotype analysis revealed a low diversity among the Chilean sequences, which may have originated from those reported in Brazil, Israel, or Cuba. Babesia caballi and T. equi were detected for the first time in Chilean thoroughbred horses.

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“…Intraspecific analysis of the sequences obtained from equids of different ecoregions in the state of Mato Grosso showed 1.95% sequence divergence for B. caballi and 2.99% for T. equi. Studies based on ribosomal (18S) genes demonstrated variability between 0.5% and 2.1% for T. equi, allowing the segregation into different genotypes (BRAGA et al, 2017;PECKLE et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Despite the endemicity of piroplasmosis among equids in Brazil, studies involving the molecular detection of the parasite in carrier animals (HEIM et al, 2007;BALDANI et al, 2010;PECKLE et al, 2013;MACHADO et al, 2012;BARROS et al, 2015;FERREIRA et al, 2016;BRAGA et al, 2017) that have also examined genetic diversity (BRAGA et al, 2017;PECKLE et al, 2018) are scarce. The aim of this study is to survey the distribution of piroplasmid (T. equi and B. caballi) parasites using molecular methods in equids from the Mato Grosso state and evaluate the interspecific genetic diversity.…”

Section: Introductionmentioning

confidence: 99%

Molecular survey and genetic diversity of piroplasmids in equids from Midwestern Brazil

Schein

Maia

Witter

et al. 2018

Rev. Bras. Parasitol. Vet.

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We evaluated the distribution of piroplasmids in equids from the Mato Grosso state in Midwestern Brazil using molecular methods and the interspecific genetic diversity. For this, 1,624 blood samples of equids from 973 farms were examined by PCR, using primer pairs that amplify a fragment of the genes rap-1 and ema-1 of Babesia caballi and Theileria equi, respectively. For molecular characterization and phylogenetic studies, 13 and 60 sequences of the rap-1 and ema-1 genes, respectively, were used to build a dendogram using maximum parsimony. B. caballi and T. equi were detected in 4.11% and 28.16% of the farms, respectively, and molecular prevalence was 2.74% for B. caballi and 25.91% for T. equi. The location of the farms and animals raised in the Pantanal ecoregion influence the probability of equids testing positive for B. caballi and T. equi . Moreover, age and herd purpose were variables significantly associated with T . equi infection. The sequences of B. caballi presented 1.95% intraspecific variability, contrasting with 2.99% in T. equi. Dendrograms for both species demonstrated the presence of subgroups with high values of support of branches. However, it is not possible to associate these groups with geographic origin and/or ecoregion.

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Molecular characterization of Theileria equi in horses from the state of Rio de Janeiro, Brazil

Cited by 22 publications

References 28 publications

Phylogenetic analysis and geographical distribution of Theileria equi and Babesia caballi sequences from horses residing in Spain

Phylogenetic analysis and geographical distribution of Theileria equi and Babesia caballi sequences from horses residing in Spain

Occurrence and Genetic Diversity of Babesia caballi and Theileria equi in Chilean Thoroughbred Racing Horses

Molecular survey and genetic diversity of piroplasmids in equids from Midwestern Brazil

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