Molecular Characterization of Thripidae (Thysanoptera) Species in Karaman, Konya and Mersin (Turkey)

Abstract

Specimens of Thripidae (Thysanoptera) species collected from Konya, Karaman and Mersin (2015-2017) were studied through molecular analyses as a part of preliminary attempts to establish a barcoding system for Thysanoptera in Turkey. The analyses included 23 species namely; Chirothrips kurdistanus zur Strassen, Chirothrips manicatus Haliday, Frankliniella intonsa (Trybom), Frankliniella occidentalis (Pergande), Frankliniella tenuicornis (Uzel), Limothrips angulicornis Jablanowski, Odontothrips confusus Priesner, Odontothrips dorycnii Priesner, Oxythrips ajugae Uzel, Taeniothrips inconsequens (Uzel), Tenothrips anatolicus (Priesner), Tenothrips discolor (Karny), Tenothrips frici (Uzel), Thermothrips mohelensis Pelikan, Thrips angusticeps Uzel, Thrips atratus Haliday, Thrips italicus Bagnall, Thrips linarius Uzel, Thrips major Uzel, Thrips meridionalis (Priesner), Thrips physapus Linnaeus, Thrips tabaci Lindeman and Thrips trehernei Priesner which were detected in various cultivated plants and weeds. The phylogenetic positions of the 9-genera, with few exceptions, to form their own clades by the 18S Ribosomal RNA data on the NJ tree. The 18S data were discussed for the first time for Th. linarius, Th. angusticeps, Th. italicus, Th. meridionalis, Th. physapus, F. tenuicornis, Ch. kurdistanus, Te. anatolicus, Te. discolor, Ther. mohelensis and Ox. ajugae species. Clearly results from the COI dataset in a single UPGMA tree separated the genera. The COI data of Li. angulicornis, Te. anatolicus, Od. confusus, Od. dorycnii Th. angusticeps, Th. atratus, Th. italicus, Th. linarius and Th. meridionalis were obtained for the first time by this study.

Exploring Aeolothrips spp. Diversity: A Morpho-Molecular Examination of Aeolothrips collaris and Aeolothrips intermedius (Thysanoptera: Aeolothripidae)

“…For the mitochondrial Cytochrome Oxidase Subunit, I (COI) gene region, the COI deg F1/R1 primers were employed, targeting a segment of approximately 350 base pairs (Timm et al 2008). The PCR protocol mirrored that used in (Şahin Negiş et al 2022). The taxonomic distances of the assembled specimens were manually corrected using the MEGA11 analysis program.…”

A modification DNA isolation method for morpho-molecular characterization: with some thrips species barcoding

“…In this phase of the study, the mitochondrial "Cytochrome Oxidase Subunit I" (COI) gene region, approximately 350 base pairs in length, was ampli ed using COI deg primers (Timm et al 2008). The PCR reaction conditions and cycling parameters for the COI gene were aligned with those established by Şahin Negiş et al (2022). Sequencing of the ampli ed products was conducted by BM Labosis, ensuring accurate and reliable determination of the genetic sequences.…”