Molecular characterization of Torque teno virus and SEN virus co-infection with HIV in patients from Southern Iran

Pirouzi, Aliyar; Bahmani, Mirzakhalil; Feizabadi, Mohammad Mehdi; Afkari, Rouhi

doi:10.1590/0037-8682-0073-2014

Cited by 25 publications

(19 citation statements)

References 29 publications

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“…Human anellovirus infections are typically detected by PCR targeting conserved regions in 3′-UTR, ORF1 and ORF2 of the viral DNA genomes [33,39,52,53]. Because of the extensive genetic variability among human anelloviruses the efficiency of PCR based methods across different genera and species is uncertain.…”

Section: Discussionmentioning

confidence: 99%

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Virome analysis of antiretroviral-treated HIV patients shows no correlation between T-cell activation and anelloviruses levels

Deng

Costa

et al. 2015

Journal of Clinical Virology

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Background Abnormally high levels of T-cell activation can persist in HIV-infected subjects despite effective anti-retroviral therapy (ART) and has been associated with negative health outcomes. The nature of the antigenic drivers or other causes of this residual T-cell activation remain uncertain. Anelloviruses are universally acquired soon after birth, resulting in persistent viremia, and considered part of the commensal human virome. Reduced immunocompetence results in increased anellovirus levels. Objectives To test whether increased levels of anelloviruses or other viruses in plasma are associated with higher levels of persistent T-cell activation during ART. Study design Two amplification methods combined with next generation sequencing were used to detect all viruses and estimate relative anellovirus levels in plasma from 19 adults on effective ART who exhibited a wide range of T-cell activation levels. Results Nucleic acids from HBV and HCV were detected in one patient each while pegivirus A (GBV-C) was found in three patients. Anellovirus DNA was detected in all patients with some individuals carrying up to eight different genotypes. Specific anellovirus genotypes or higher level of co-infections were not detected in subjects with higher levels of T-cell activation. No association was detected between relative plasma anellovirus DNA levels and the percentage of activated CD4 or CD8 T cells. Conclusions Human anelloviruses were detected in all HIV suppressed subjects, exhibited a wide range of viremia levels, and were genetically highly diverse. The level of persistent T-cell activation was not correlated with the level of viremia or genotypes present indicating that anellovirus antigens are unlikely to be a dominant source of antigens driving chronic T-cell activation.

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Section: Discussionmentioning

confidence: 99%

“…HIV-infected individuals showed higher detection rate and estimated titer of human anelloviruses than healthy blood donors [33–35]. Anellovirus loads were higher in HIV-infected individuals with low versus high CD4+ T-cell counts [31,36–38].…”

Section: Introductionmentioning

confidence: 99%

Virome analysis of antiretroviral-treated HIV patients shows no correlation between T-cell activation and anelloviruses levels

Deng

Costa

et al. 2015

Journal of Clinical Virology

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“…A small number of studies have examined the prevalence and genetic diversity of TTV in human and nonhuman Middle Eastern populations [10,12,[23][24][25]. One of these studies reported a prevalence of 90% (27/30) among Jordanian human subjects [10].…”

Section: Introductionmentioning

confidence: 99%

The Molecular Epidemiology and Phylogeny of Torque Teno Virus (TTV) in Jordan

Sarairah

Bdour

Gharaibeh

2020

Viruses

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Torque teno virus (TTV) is the most common component of the human blood virobiota. Little is known, however, about the prevalence of TTV in humans and the most common farm domesticates in Jordan, or the history and modality of TTV transmission across species lines. We therefore tested sera from 396 Jordanians and 171 farm animals for the presence of TTV DNA using nested 5′-UTR-PCR. We then performed phylogenetic, ordination and evolutionary diversity analyses on detected DNA sequences. We detected a very high prevalence of TTV in Jordanians (~96%); much higher than in farm animal domesticates (~29% pooled over species). TTV prevalence in the human participants is not associated with geography, demography or physical attributes. Phylogenetic, ordination and evolutionary diversity analyses indicated that TTV is transmitted readily between humans across the geography of the country and between various species of animal domesticates. However, the majority of animal TTV isolates seem to derive from a single human-to-animal transmission event in the past, and current human-animal transmission in either direction is relatively rare. In conclusion, animal TTV in Jordan is historically derived from human variants; however, ongoing human-animal TTV exchange is minimal and zoonotic infection seems to be of limited importance.

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“…[7] SENV is non-enveloped, round, icosahedral symmetry with a single-stranded DNA genome. [8] The genome is circular, single-stranded of about 1.8-3.8 kb. SENV and Torque Teno Virus show a similar genomic organization; the nucleotide sequence had identified two dominant, partially overlapping open reading frames (ORFs), ORF1, and ORF2.…”

mentioning

confidence: 99%

Molecular and Genotypic Study of Senv-D Virus Coinfection in Β-Thalassemic Patients Infected With the Hepatitis C Virus in Iraq

Al-Ouqaili¹

2018

IJGP

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Objective and Background: SEN virus has identified as a putative new hepatitis virus coinfected with hepatitis C in thalassemic patients. The study aimed to measure viremia with SEN virus among thalassemic patients infected and non-infected with hepatitis C virus (HCV). Further, to detect the genotype D and the genetic alterations in the internal transcribed spacer domains/regions of SENV-D genotypes. Patients and Methods: A total of 200 β-thalassemic patients were included. HCV-RNA and SEN-DNA are extracting automatically. Then, amplification for SEN-DNA by conventional polymerase chain reaction (PCR) was achieved. Furthermore, HCV-RNA was amplified using real-time PCR (RT-PCR). Sequencing analysis was also done for 14 samples of amplified SENV-D DNA. Results: Out of 100 positive ELISA-anti-HCV antibodies which were definite for RT-PCR, the mean viral load in these patients was 545806 ± 1,009,799 IU/ml (1,997,176 ±3,802,206) copies/ml. There was a significant increase in SENV-D in thalassemia patients without HCV infection (59%) than those in thalassemia patients with HCV infection (44%) and healthy subjects (7%). In a sequencing study, 11 samples were infected with D genotype only while three from thalassemia patients group were coinfected with D and H genotypes. Conclusions: The occurrence rate of SEN-V DNA in thalassemia patients is very high in Iraq. Further, SEN-V-D genotype is more prevalent, and the sequences of SEN-V-D nucleotides in persons with coinfection are the same sequences of persons that have an infection with SEN-V-D alone. The most countries which have similar sequences to Iraqi SENV-D genotype sequence are Iran followed by China, Japan, Brazil, and the United States of America.

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Molecular characterization of Torque teno virus and SEN virus co-infection with HIV in patients from Southern Iran

Cited by 25 publications

References 29 publications

Virome analysis of antiretroviral-treated HIV patients shows no correlation between T-cell activation and anelloviruses levels

Virome analysis of antiretroviral-treated HIV patients shows no correlation between T-cell activation and anelloviruses levels

The Molecular Epidemiology and Phylogeny of Torque Teno Virus (TTV) in Jordan

Molecular and Genotypic Study of Senv-D Virus Coinfection in Β-Thalassemic Patients Infected With the Hepatitis C Virus in Iraq

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