Molecular characterization of TRIB1 gene and its role in regulation of steroidogenesis in bos grunniens granulosa cells

Zhao, Dan; Fan, Yiling; Xiong, Xianrong; Yin, Shi; Fu, Wei; Ma, Yan; Yue, Yongqi; Zhao, Zhidong; Li, Jian; Xiong, Yan

doi:10.1016/j.theriogenology.2022.07.012

Cited by 10 publications

(2 citation statements)

References 54 publications

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“…GCs regulate the development and blockade of follicles through various factors, including gonadotropin receptors, steroid hormones, as well as various growth factors, and cytokines (62)(63)(64). Steroid hormones, including estrogen, progestogen, and androgen, directly regulate the maturation of follicles (65). Ras protein, as a GTPase and a molecular switch in signaling pathways, regulates cell proliferation, survival, growth, migration, differentiation, or cytoskeletal dynamics (66).…”

Section: Discussionmentioning

confidence: 99%

Differential age-related transcriptomic analysis of ovarian granulosa cells in Kazakh horses

Ren,

Wang,

Zeng

et al. 2024

Front. Endocrinol.

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IntroductionThe Kazakh horse, renowned for its excellence as a breed, exhibits distinctive reproductive traits characterized by early maturity and seasonal estrus. While normal reproductive function is crucial for ensuring the breeding and expansion of the Kazakh horse population, a noteworthy decline in reproductive capabilities is observed after reaching 14 years of age.MethodsIn this study, ovarian granulosa cells (GCs) were meticulously collected from Kazakh horses aged 1, 2, 7, and above 15 years old (excluding 15 years old) for whole transcriptome sequencing.ResultsThe analysis identified and selected differentially expressed mRNAs, lncRNAs, miRNAs, and circRNAs for each age group, followed by a thorough examination through GO enrichment analysis. The study uncovered significant variations in the expression profiles of mRNAs, lncRNAs, miRNAs, and circRNAs within GCs at different stages of maturity. Notably, eca-miR-486-3p and miR-486-y exhibited the highest degree of connectivity. Subsequent GO, KEGG, PPI, and ceRNA network analyses elucidated that the differentially expressed target genes actively participate in signaling pathways associated with cell proliferation, apoptosis, and hormonal regulation. These pathways include but are not limited to the MAPK signaling pathway, Hippo signaling pathway, Wnt signaling pathway, Calcium signaling pathway, Aldosterone synthesis and secretion, Cellular senescence, and NF-kappa B signaling pathway—essentially encompassing signal transduction pathways crucial to reproductive processes.DiscussionThis research significantly contributes to unraveling the molecular mechanisms governing follicular development in Kazakh horses. It establishes and preliminarily validates a differential regulatory network involving lncRNA-miRNA-mRNA, intricately associated with processes such as cell proliferation, differentiation, and apoptosis and integral to the developmental intricacies of stromal follicles. The findings of this study provide a solid theoretical foundation for delving deeper into the realm of reproductive aging in Kazakh mares, presenting itself as a pivotal regulatory pathway in the context of horse ovarian development.

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Section: Discussionmentioning

confidence: 99%

Differential age-related transcriptomic analysis of ovarian granulosa cells in Kazakh horses

Ren,

Wang,

Zeng

et al. 2024

Front. Endocrinol.

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“…IHC was performed as described in the previous study [23]. In brief, testicular samples from fetal, juvenile, and adult yaks and adult cattle-yaks (n = 3, respectively) were collected and immediately fixed with 4% paraformaldehyde.…”

Section: Immunohistochemistry (Ihc) Analysismentioning

confidence: 99%

The Biological Characteristics and Differential Expression Patterns of TSSK1B Gene in Yak and Its Infertile Hybrid Offspring

Zhu

Pan

Fei

et al. 2023

Animals

Self Cite

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This study aimed to investigate the spatially and temporally expressed patterns and biological characteristics of TSSK1B in male yaks and explore the potential correlation between TSSK1B and male sterility of the yak hybrid offspring (termed cattle–yak). First, the coding sequence (CDS) of TSSK1B was cloned by RT-PCR, and bioinformatics analysis was conducted with relevant software. Quantitative real-time PCR (RT-qPCR) was employed to detect the expression profile of TSSK1B in various tissues of male adult yaks, the spatiotemporal expression of TSSK1B in different stages of yak testes, and the differential expression of TSSK1B between yak and cattle–yak testes. The cellular localization of TSSK1B was determined by immunohistochemistry (IHC). Furthermore, the methylation status of the TSSK1B promoter region was analyzed by bisulfite-sequencing PCR (BSP). The results showed that TSSK1B was 1235 bp long, including 1104 bp of the CDS region, which encoded 367 amino acids. It was a conserved gene sharing the highest homology with Bos mutus (99.67%). In addition, the bioinformatics analysis revealed that TSSK1B was an unstable hydrophilic protein mainly containing the alpha helix of 34.06% and a random coil of 44.41%, with a transmembrane structure of 29 amino acids long. The RT-qPCR results demonstrated that TSSK1B was specifically expressed in yak testes compared with that in other tissues and especially highly expressed in adult yak testes. On the contrary, TSSK1B was hardly expressed in the testis of adult cattle–yak. IHC confirmed that TSSK1B protein was more strongly expressed in the testes of adult yaks than in their fetal and juvenile counterparts. Interestingly, nearly no expression was observed in the testes of cattle–yak compared with the corresponding testes of yak. Bisulfite-sequencing PCR (BSP) revealed that the methylated CpG sites in the TSSK1B promoter region of cattle–yak was significantly higher than that in the yak. Taken together, this study revealed that TSSK1B was specifically expressed in yak testes and highly expressed upon sexual maturity. Moreover, the rare expression in cattle–yak may be related to the hypermethylation of the promoter region, thereby providing a basis for further studies on the regulatory mechanism of TSSK1B in male cattle–yak sterility.

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TMT-based quantitative proteomics analysis reveals the differential proteins between fresh and frozen-thawed sperm of yak (Bos grunniens)

Fan

Li²,

Guo

et al. 2023

Theriogenology

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Molecular characterization of TRIB1 gene and its role in regulation of steroidogenesis in bos grunniens granulosa cells

Cited by 10 publications

References 54 publications

Differential age-related transcriptomic analysis of ovarian granulosa cells in Kazakh horses

Differential age-related transcriptomic analysis of ovarian granulosa cells in Kazakh horses

The Biological Characteristics and Differential Expression Patterns of TSSK1B Gene in Yak and Its Infertile Hybrid Offspring

TMT-based quantitative proteomics analysis reveals the differential proteins between fresh and frozen-thawed sperm of yak (Bos grunniens)

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