Molecular characterization of tumor associated antigen in mice exposed to a hepatocarcinogen

Alam, Anis; Singha, Laishram Indira; Singh, Vinod

doi:10.1007/s11010-005-4034-8

Cited by 10 publications

(5 citation statements)

References 19 publications

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“…DEN was administered by intravenous (i.v.) route[1112] at weekly intervals from week zero (start of the experiment) until the end of experiment (16 or 28 weeks). The exposures to the HPE (either AT or CC) were initiated from week 10 until the end of experiment (weeks 16 or 28) by thrice weekly intraperitoneal (i.p.)…”

Section: Methodsmentioning

confidence: 99%

Inhibitory potential of methanolic extracts ofAristolochia tagalaandCurcuma caesiaon hepatocellular carcinoma induced by diethylnitrosamine in BALB/c mice

Hadem¹,

Sharan²,

Kma³

2014

J Carcinog

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Context:Aristolochia tagala (AT) and Curcuma caesia (CC) have been used traditionally by local herbal practitioners for cancer treatment and as chief ingredients of many polyherbal formulations for various types of ailments. However, there is void in scientific study to evaluate their anti-cancer property.Aims:The aim of this study was to evaluate the anti-carcinogenic properties of the crude methanolic extracts of roots of AT and rhizomes of CC in BALB/c mice exposed to a hepatocarcinogen, diethylnitrosamine (DEN).Settings and Design:(I) Toxicity of herbal plant extracts (HPE); (II) Anticancer studies; (III) Histological studies; and (IV) Biochemical studies.Materials and Methods:To evaluate the effects of these two HPE either alone or following DEN exposure, serum transaminases (aspartate aminotransferase [AST], alanine aminotransferase [ALT]), alkaline phosphatase (ALP), and cancer marker enzyme acetylcholine esterase (AChE) were assayed in mice. In addition, histological study was also carried out under similar conditions. The antioxidant potentials of the HPE were evaluated by monitoring the activity of antioxidant enzymes and metabolites, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione (GSH).Statistical Analysis Used:Statistical analysis was performed by GraphPad Prism 6 Software using one-way analysis of variance followed by the Tukey's multiple comparisons test. Significance was set at P < 0.05.Results:Our findings show that DEN administration elevated AST, ALT, ALP, and AChE activities. CC or AT extracts attenuated the increased activities of these marker enzymes. The activities of antioxidant enzymes, which were decreased following DEN administration, were significantly increased in mice treated with CC or AT.Conclusions:The present study clearly documents anticarcinogenic and antioxidant properties of AT and CC in DEN-induced mouse liver cancer in vivo.

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Section: Methodsmentioning

confidence: 99%

Inhibitory potential of methanolic extracts ofAristolochia tagalaandCurcuma caesiaon hepatocellular carcinoma induced by diethylnitrosamine in BALB/c mice

Hadem¹,

Sharan²,

Kma³

2014

J Carcinog

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“…The GGT assay was carried out by following the method described in our recent communication [8,9]. In brief, to 0.2 ml of γ -glutamyl-p-nitroanilide (0.005 M, pH 8.0) in a cuvette, 0.2 ml of glycylglycine (0.1 M, pH 8.0) and 0.6 ml of Tris-HCl buffer (0.1 M, pH 8.0) were added.…”

Section: γ -Glutamyl Transpeptidase (Ggt) Assaymentioning

confidence: 99%

Radiomodulatory effect of liposome encapsulated AK-2123 on tumor in mice exposed to hepatocarcinogen

et al. 2005

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An attempt was made to evaluate the whole body gamma-radiation effect on tumor in the presence of free and liposome encapsulated AK-2123, a hypoxic cell radiosensitizer that has widely been used in combination with a number of cancer therapies such as thermotherapy, chemotherapy and radiotherapy. Entrapment efficiency of AK-2123 into liposome was determined by LASER Raman spectroscopy. Cancer induction in mice was carried out by repeated exposure of N-nitrosodiethylamine (DEN) in combination with partial hepatectomy. Parameters such as marker enzymes activities (GGT and AChE), rates of nucleic acid synthesis, viability modification factor and the histology of liver tissues monitored, supported the induction of cancer in liver. In addition, the effect of free as well as liposome encapsulated AK-2123 on haemopoietic parameters were also studied. It was observed that AK-2123 after incorporation into liposome afforded more efficient radiomodulatory effects than that of free AK-2123 as determined by the above-mentioned parameters. Neither free AK-2123 nor liposome encapsulated AK-2123 showed any detectable toxic effects on the mice. Thus, it is seen that treatment of cancer with a combination of radiation, a radiomodifier and a drug delivery system, opens a wide scope for exploitation for the improvement of existing cancer therapies.

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“…Szemes and szamado (1991) found that rats received sodium nitrite showed histopathological changes in striated muscles, liver and kidney. Also, Alam et al (2005) observed that mice exposed to nitrosodibutylamine showed cell destruction and extensive necrosis.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Aquaeous Green Tea Extract on Toxicity Caused by Sodium Nitrite

2011

Assiut Veterinary Medical Journal

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Molecular characterization of tumor associated antigen in mice exposed to a hepatocarcinogen

Cited by 10 publications

References 19 publications

Inhibitory potential of methanolic extracts ofAristolochia tagalaandCurcuma caesiaon hepatocellular carcinoma induced by diethylnitrosamine in BALB/c mice

Inhibitory potential of methanolic extracts ofAristolochia tagalaandCurcuma caesiaon hepatocellular carcinoma induced by diethylnitrosamine in BALB/c mice

Radiomodulatory effect of liposome encapsulated AK-2123 on tumor in mice exposed to hepatocarcinogen

Protective Effect of Aquaeous Green Tea Extract on Toxicity Caused by Sodium Nitrite

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