Molecular characterization of tumor necrosis α-induced protein 6 and its human chorionic gonadotropin-dependent induction in theca and mural granulosa cells of equine preovulatory follicles

Sayasith, Khampoune; Doré, Monique; Sirois, Jean

doi:10.1530/rep.1.01200

Cited by 16 publications

(16 citation statements)

References 49 publications

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“…In mares, TNFAIP6 is also expressed in theca cells, which may reflect other roles of TNFAIP6 in equine preovulatory follicles [23]. Here, we demonstrated expression of TNFAIP6 mRNA in 235 TNFAIP6 mRNA IN PREOVULATORY FOLLICLE both cumulus and mural granulosa cells following gonadotropinstimulation in vivo and in vitro.…”

Section: Nagyova Et Al 234supporting

confidence: 50%

“…Even though the length of the ovulatory process and involvement of the MGC in formation of a stalk of expanded cells in gilts [28] are similar to mares, we have not been able to confirm a biphasic pattern of TNFAIP6 gene expression in the pig. The second rise in TNFAIP6 mRNA in mares coincides with activation of cyclooxygenase 2 and may thus reflect an assumed role of prostaglandin in the production of TNFAIP6 [23]. Alternatively, this pattern of TNFAIP6 expression may be typical of the monoovulatory species.…”

Section: Nagyova Et Al 234mentioning

confidence: 95%

“…Under these culture conditions, neither the TNFAIP6-HC species nor HCs were present in the matrix extract, indicating that the A38 antibody successfully blocked TNFAIP6/ IαI interaction and, consequently, HC linkage to HA [17]. In rodent follicles, expression of TNFAIP6 mRNA appears to be limited to cumulus cells and granulosa cells, in which the expression decreases from the inner to the outer layers [8].In mares, TNFAIP6 is also expressed in theca cells, which may reflect other roles of TNFAIP6 in equine preovulatory follicles [23]. Here, we demonstrated expression of TNFAIP6 mRNA in 235 TNFAIP6 mRNA IN PREOVULATORY FOLLICLE both cumulus and mural granulosa cells following gonadotropinstimulation in vivo and in vitro.…”

mentioning

confidence: 93%

mentioning

confidence: 95%

“…Recently, gonadotropin-dependent biphasic induction of TNFAIP6 during ovulation was demonstrated in mares, the pattern of which different from that observed in rodents [23]. Moreover, Kawashima et al [24] demonstrated a dramatic increase in TNFAIP6 gene expression in OCCs obtained from eCG (72 h)-treated gilts, but before application of hCG.…”

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confidence: 98%

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Expression of Tumor Necrosis Factor Alpha-Induced Protein 6 Messenger RNA in Porcine Preovulatory Ovarian Follicles

2009

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Abstract. It has been shown previously that tumor necrosis factor alpha-induced protein 6 (TNFAIP6) is essential for formation of the cumulus extracellular matrix and female fertility. Therefore, we studied the expression of TNFAIP6 mRNA in porcine preovulatory follicles. In addition, we asked whether the expression of TNFAIP6 mRNA changes in mural granulosa cells (MGCs) during the periovulatory period or after culture of oocyte-cumulus complexes (OCCs) or MGCs in vitro. Mural granulosa cells obtained from follicles on day 12 (D 12) and day 15 (D 15) of the estrous cycle, eCG-stimulated follicles, follicles at 4-32 h after hCG stimulation and MGCs and OCCs obtained from immature gilts and cultured for 0-44 h in vitro with gonadotropins were used for extraction of total RNA and assessment of the relative abundance (RA) of TNFAIP6 mRNA by reverse transcription-polymerase chain reaction (RT-PCR). The levels of TNFAIP6 mRNA were low in the follicles on D 12 and D 15 of the estrous cycle and at 66 h after eCG stimulation but were significantly increased at 4 h after hCG. The high level of TNFAIP6 expression was maintained until 16 h after hCG stimulation and gradually decreased at 24 and 32 h after hCG. During in vitro culture, FSH/LH-induced TNFAIP6 mRNA was expressed in both OCCs and MGCs in a similar temporal pattern as seen in vivo. We conclude that TNFAIP6 expression in the pig, like other species, increases in preovulatory follicles following the LH (hCG) surge. The OCC and MGC display similar patterns of TNFAIP6 expression under both in vivo and in vitro conditions. Key words: mRNA TNFAIP6, Mural granulosa cells, Oocyte-cumulus complex, Pig (J. Reprod. Dev. 55: [231][232][233][234][235] 2009) uteinizing hormone (LH)-induced ovulation is a complex, inflammation-like process that results in the release of a fertilizable oocyte from the ovary in the oviduct [1]. In most mammals, before ovulation, cumulus cells synthesize a large amount of hyaluronan (HA), which is organized in a highly hydrated, mucoelastic matrix [2]. Several proteins have been identified as being essential for matrix formation and stability of the mouse cumulus-oocyte complex; among them serum-derived inter-alpha-trypsin inhibior (IαI) [3] and tumor necrosis factor alpha-induced protein 6 (TNFAIP6) [4]. Covalent transfer of heavy chains (HCs) of IαI to HA is a prerequisite for expansion of mouse oocyte-cumulus complexes (OCCs) [5], and this also occurs in pig OCCs matured in vivo or cultured in vitro with pig serum or pig follicular fluid [6]. Several lines of evidence suggest that TNFAIP6 participates in transfer of HCs to HA and plays an essential role in regulation of female fertility. TNFAIP6 is produced in rodent ovaries by cumulus and granulosa cells after an ovulatory stimulus [7][8][9][10] in parallel to HA synthesis by OCCs [7,11,12]. It is an inflammation-associated protein with the ability to bind HA, IαI and other ligands and participate in extracellular matrix formation and remodeling [13][14][15]. In the OCCs of TNFAIP6 null mi...

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Section: Nagyova Et Al 234supporting

confidence: 50%

Section: Nagyova Et Al 234mentioning

confidence: 95%

mentioning

confidence: 93%

mentioning

confidence: 95%

mentioning

confidence: 98%

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Expression of Tumor Necrosis Factor Alpha-Induced Protein 6 Messenger RNA in Porcine Preovulatory Ovarian Follicles

2009

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Gonadotropin‐dependent regulation of the prostaglandin E2 receptor in equine preovulatory follicles during the ovulatory process in mares

Sayasith

Bouchard

Doré

et al. 2008

Molecular Reproduction Devel

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The objectives of the study were to clone the primary structure of the prostaglandin E2 receptor subtype 2 (PTGER2) cDNA and to characterize its regulation in equine follicles during gonadotropin-induced ovulation. Results from DNA isolation indicated that the equine PTGER2 cDNA encodes a predicted 353-amino acid protein, which is highly similar (76-85%) to known mammalian homologues. The regulation of PTGER2 was studied by semi-quantitative RT-PCR/Southern blot using preparations of theca interna and mural granulosa cells isolated from equine follicles 0-39 hr post-treatment with human chorionic gonadotropin (hCG). Results indicated that a significant increase of PTGER2 mRNA occurred at 24 and 39 hr post-hCG in granulosa cells, and 30 and 33 hr post-hCG in theca cells (P < 0.05). Immunohistochemical staining and immunoblotting performed on equine follicular samples showed a corresponding increase of PTGER2 protein in both cell types after treatment with hCG. Levels of PTGER2 mRNA were also high in uterus, thymus and spleen, but moderate to low in other tested tissues. In the ovary, the expression of PTGER4 mRNA was observed and predominantly occurred in granulosa cells, with highest abundance of transcripts observed at 12 and 39 hr post-hCG. Thus, this study reports for the first time in mares that the ovulatory process is accompanied by the gonadotropin-dependent up-regulation of PTGER2 and PTGER4, which may in turn regulate PGE2-mediated preovulatory effects.

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Ankyrin‐repeat and SOCS box‐containing protein 9 (ASB9) regulates ovarian granulosa cells function and MAPK signaling

Nosratpour

Ndiaye

2021

Molecular Reproduction Devel

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Ankyrin‐repeat and SOCS box‐containing proteins (ASB) interact with the elongin B‐C adapter via their SOCS box domain and with the cullin and ring box proteins to form E3 ubiquitin ligase complexes within the protein ubiquitination pathway. ASB9 in particular is a differentially expressed gene in ovulatory follicles (OFs) induced by the luteinizing hormone (LH) surge or hCG injection in ovarian granulosa cells (GC) while downregulated in growing dominant follicles. Although ASB9 has been involved in biological processes such as protein modification, the signaling network associated with ASB9 in GC is yet to be fully defined. We previously identified and reported ASB9 interactions and binding partners in GC including PAR1, TAOK1, and TNFAIP6/TSG6. Here, we further investigate ASB9 effects on target binding partners regulation and signaling in GC. CRISPR/Cas9‐induced inhibition of ASB9 revealed that ASB9 regulates PAR1, TAOK1, TNFAIP6 as well as genes associated with proliferation and cell cycle progression such as PCNA, CCND2, and CCNE2 while CCNA2 was not affected. Inhibition of ASB9 was also associated with increased GC number and decreased caspase3/7 activity, CASP3 expression, and BAX/BCL2 ratio. Furthermore, ASB9 induction in OF in vivo 24 h post‐hCG is concomitant with a significant decrease in phosphorylation levels of MAPK3/1 while pMAPK3/1 levels increased following ASB9 inhibition in GC in vitro. Together, these results provide strong evidence for ASB9 as a regulator of GC activity and function by modulating MAPK signaling likely through specific binding partners such as PAR1, therefore controlling GC proliferation and contributing to GC differentiation into luteal cells.

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Molecular characterization of tumor necrosis α-induced protein 6 and its human chorionic gonadotropin-dependent induction in theca and mural granulosa cells of equine preovulatory follicles

Cited by 16 publications

References 49 publications

Expression of Tumor Necrosis Factor Alpha-Induced Protein 6 Messenger RNA in Porcine Preovulatory Ovarian Follicles

Expression of Tumor Necrosis Factor Alpha-Induced Protein 6 Messenger RNA in Porcine Preovulatory Ovarian Follicles

Gonadotropin‐dependent regulation of the prostaglandin E2 receptor in equine preovulatory follicles during the ovulatory process in mares

Ankyrin‐repeat and SOCS box‐containing protein 9 (ASB9) regulates ovarian granulosa cells function and MAPK signaling

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